LOCUS       sequence-39519-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A Drosophila GFP reporter transgene vector for site-specific 
            integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Williams TM, Selegue JE, Werner T, Gompel N, Kopp A, Carroll SB
  TITLE     The regulation and evolution of a genetic switch controlling 
            sexually dimorphic traits in Drosophila.
  JOURNAL   Cell. 2008 Aug 22;134(4):610-23. doi: 10.1016/j.cell.2008.06.052.
  PUBMED    18724934
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.06"; journalName: "Cell"; date: "2008-08-22- 
            22"; volume: "134"; issue: "4"; pages: "610-23"
COMMENT     Sequence Label: pS3aG
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(356..358,359..361,362..874)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(401..422)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(662..681)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctcattccgt ggactgagcc atcgactccg ctgctcagaa tagatctggt ctagagcccg
       61 ggcgaattcg ccggcgcgcc ggtaccaagg ccggccaaat ttaaataacc tgcaggaagc
      121 gatcgcaacc gcggaagcta gcggatccct cgaggagcgc cggagtataa atagaggcgc
      181 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
      241 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagcca agctgatcct
      301 ctagggtacg cgctagagtc gagaggcctg tttaaacgat ccaccggtcg ccaccatggt
      361 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      421 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      481 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      541 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      601 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      661 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      721 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      781 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      841 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      901 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca acc
//
