LOCUS       sequence-395196-        5665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  in vitro transcription of RNA encoding HA-tagged Acvr1l.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Tajer B, Dutko JA, Little SC, Mullins MC
  TITLE     BMP heterodimers signal via distinct type I receptor class 
            functions.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Apr 13;118(15):e2017952118. doi: 
            10.1073/pnas.2017952118.
  PUBMED    33827919
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-04-13"; volume: "118(15):e2017952118. 
            doi"; pages: " 10.1073/pnas.2017952118"
COMMENT     Sequence Label: acvr1l-HA
FEATURES             Location/Qualifiers
     source          1..5665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..50
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     32..49
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1647..1673
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1714..1848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1738..1757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1792..1811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1961..1981)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1961..1979)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2000..2016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2000..2016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2013..2035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2024..2040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2048..2054,2055..2072,2073..2078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2093..2114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2231..2248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2402..2990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2482..2501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3161..3952,3953..4021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3784..3803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4022..4126)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4152..4607)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4289..4310)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4501..4520
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4678..5662
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cattctgcct ggggacgtcg gagcaagctt gatttaggtg acactataga atacaagcta
       61 cttgttcttt ttgcaggatc cggcagcgct gagagtcact tgaggagttg tacaatgcat
      121 tgaaccaaat ggggcattgc agcacccaaa tcatcatcct gttcctgctt cagttcttac
      181 agacatcagc taaagatgtc tccattgact gcatgtgtgt cggcagtgac tgtaatgagc
      241 agcagtgtac tggtgaccag tgttacacct ccgttatcat tagcaatgat gtgacgacgt
      301 tcaagcgggg ctgcttgatc gggccggcga gcaagcgcat gacctgctcc gcaacagctt
      361 ctgctagtca tgtggtagaa tgctgttctc aacacatgtg caacgccaac gtctccaaag
      421 agaccctact tcgactgctg ctcacaagtc cagaagaaaa gaagactgtt cattaccgcg
      481 tggaaatgtt ggttctgttt gtgttggggc cgtttgtggt tctgggtctg ctgtcttttc
      541 tggctttgct ggtgtgccgt cgactccatc atgggcgtct ggagagactg cacgagtttg
      601 acactgaaca gggggccatc gatgggctta tcgcgtctaa tgtcggagac agcacacttg
      661 cggatctgat ggatcactcc tgcacttcag gcagtggttc aggactgccc ttcctggttc
      721 agagaacggt tgcgcggcag atcagcctgg tggagtgtgt tggtaaagga cggtacggtg
      781 aagtgtggag aggtcaatgg caaggagaaa atgtagccgt gaagatcttt tcctctagag
      841 atgagaagtc atggtttcga gaaacagaaa tttacaacac tgttctgcta cgacatgaaa
      901 atatattagg cttcatggct tctgacatga cctcccgaaa ctctagcact cagctgtggc
      961 tgatcacaca ctatcacgag aatggctctc tgtatgacta cctgcagcgt gtggctgtgg
     1021 agatggcaga tggactgcac atggcggctt cgattgccag cgggctggtg cacctgcaca
     1081 cggagatctt tggcacggag ggcaaaccgg ccatcgctca cagagacctg aagagcaaga
     1141 acatccttgt gaagaaagat ttgcagtgct gcatcgctga cctgggtctg gcagtaacac
     1201 acacgcagtc tgataatcag cttgatgtgg gaaataatcc taaagtggga accaaacgct
     1261 acatggcacc ggaggttcta gatgagacca ttcagacgga ctgttttgac gcctataaga
     1321 gggtggatat ctgggccttt gggttggtgc tgtgggagat cgcacgcaga accatcagca
     1381 atggaattgt agaggaatac aagccgcctt tctatgacct ggttcctaat gatcccagct
     1441 ttgacgacat gaggaaagtg gtttgtgtgg agcagcaaag gccattcatt cccaaccgct
     1501 ggttttcaga tcctaccctg tctgctctgg tgaagctgat gaaagagtgc tggtaccaga
     1561 acccctcggc tcgtctcact gccctgcgca tcaaaaagac tctggataaa atccacagtt
     1621 cactggagaa gggcaaaacc gactgctacc catacgatgt gccagattac gcttgactcg
     1681 agcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     1741 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1801 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1861 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     1921 gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     1981 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2041 ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc taatgagtga
     2101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2821 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4141 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4201 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4261 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4321 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4381 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4441 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4501 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4561 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     4621 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc
     4681 gaccgccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt ggatctggac
     4741 ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt ggatctggac
     4801 cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc ggacctggca
     4861 ctgtgccaac tggggagggg tctacttggc acggtgccaa gtttgaggag gggtcttggc
     4921 cctgtgccaa gtccgccata ttgaattggc atggtgccaa taatggcggc catattggct
     4981 atatgccagg atcaatatat aggcaatatc caatatggcc ctatgccaat atggctattg
     5041 gccaggttca atactatgta ttggccctat gccatatagt attccatata tgggttttcc
     5101 tattgacgta gatagcccct cccaatgggc ggtcccatat accatatatg gggcttccta
     5161 ataccgccca tagccactcc cccattgacg tcaatggtct ctatatatgg tctttcctat
     5221 tgacgtcata tgggcggtcc tattgacgta tatggcgcct cccccattga cgtcaattac
     5281 ggtaaatggc ccgcctggct caatgcccat tgacgtcaat aggaccaccc accattgacg
     5341 tcaatgggat ggctcattgc ccattcatat ccgttctcac gccccctatt gacgtcaatg
     5401 acggtaaatg gcccacttgg cagtacatca atatctatta atagtaactt ggcaagtaca
     5461 ttactattgg aagtacgcca gggtacattg gcagtactcc cattgacgtc aatggcggta
     5521 aatggcccgc gatggctgcc aagtacatcc ccattgacgt caatggggag gggcaatgac
     5581 gcaaatgggc gttccattga cgtaaatggg cggtaggcgt gcctaatggg aggtctatat
     5641 aagcaatgct cgtttaggga accgc
//
