LOCUS       sequence-395199-       11920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent based sex separator in Aedes aegypti (SEPARATOR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Weng SC, Antoshechkin I, Marois E, Akbari OS
  TITLE     Efficient Sex Separation by Exploiting Differential Alternative 
            Splicing of a Dominant Marker in Aedes aegypti.
  JOURNAL   bioRxiv. 2023 Jun 16:2023.06.16.545348. doi: 
            10.1101/2023.06.16.545348. Preprint.
  PUBMED    37398094
REFERENCE   2  (bases 1 to 11920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jun 16:2023.06.16.545348. doi: 10.1101/2023.06.16.545348. 
            Preprint."
COMMENT     Sequence Label: Vector 1174D
FEATURES             Location/Qualifiers
     source          1..11920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1037..1071
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1541..1610
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    1707..2092
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        2099..2581
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        2585..3176
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             join(7713..7715,7716..7718,7719..8393)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(7896..7916)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8114..8133
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8308..8331
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             join(8396..8398,8399..8401,8402..9115)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8396..8398,8399..8401,8402..9112)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8441..8462)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8702..8721)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9049..9070
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9237..9358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9274..9293)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9328..9347
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9373..9758
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     repeat_region   10076..10138
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10146..10163)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10146..10162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10155..10177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10304..10759
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10391..10410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10601..10622
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10785..10889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10890..10958,10959..11750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11108..11127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     1081 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     1141 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     1201 actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     1261 ttaactcata cgataattat attgttattt catgttctac ttacgtgata acttattata
     1321 tatatatttt cttgttatag atatctgtcg acatgcccgc cgtgaccgtc gagaacccgc
     1381 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
     1441 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
     1501 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
     1561 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
     1621 acatcgtcga cgggcccgcg tgcaaaagcg tgtttttttg cagtgcaaaa aagttggtgg
     1681 tggggaggcc accgagtatg ccgcggaatt gatcggctaa atggtatggc aagaaaaggt
     1741 atgcaatata ataatctttt attgggtatg caacgaaaat ttgtttcgtc aacgtatgca
     1801 atatttttta ttaaaagagg gtatgcaatg tattttatta aaaacgggta tgcaatataa
     1861 taatctttta ttgggtatgc aacgaaaatt tgtttcgtca aagtatgcaa tattttttat
     1921 taaaagaggg tatgcaatgt attttattaa aaacgggtat gcaataaaaa attatttggt
     1981 ttctctaaaa agtatgcagc acttattttt tgataaggta tgcaacaaaa ttttactttg
     2041 ccgaaaatat gcaatgtttt tgcgaataaa ttcaacgcac acttattacg tgaggtaccg
     2101 cgtaaaacac aatcaagtat gagtcataag ctgatgtcat gttttgcaca cggctcataa
     2161 ccgaactggc tttacgagta gaattctact tgtaacgcac gatcagtgga tgatgtcatt
     2221 tgtttttcaa atcgagatga tgtcatgttt tgcacacggc tcataaactc gctttacgag
     2281 tagaattcta cgtgtaacgc acgatcgatt gatgagtcat ttgttttgca atatgatatc
     2341 atacaatatg actcatttgt ttttcaaaac cgaacttgat ttacgggtag aattctactt
     2401 gtaaagcaca atcaaaaaga tgatgtcatt tgtttttcaa aactgaactc gctttacgag
     2461 tagaattcta cgtgtaaaac acaatcaaga aatgatgtca tttgttataa aaataaaagc
     2521 tgatgtcatg ttttgcacat ggctcataac taaactcgct ttacgggtag aattctacgc
     2581 gcgtcgatgt ctttgtgatg cgcgcgacat ttttgtaggt tattgataaa atgaacggat
     2641 acgttgcccg acattatcat taaatccttg gcgtagaatt tgtcgggtcc attgtccgtg
     2701 tgcgctagca tgcccgtaac ggacctcgta cttttggctt caaaggtttt gcgcacagac
     2761 aaaatgtgcc acacttgcag ctctgcatgt gtgcgcgtta ccacaaatcc caacggcgca
     2821 gtgtacttgt tgtatgcaaa taaatctcga taaaggcgcg gcgcgcgaat gcagctgatc
     2881 acgtacgctc ctcgtgttcc gttcaaggac ggtgttatcg acctcagatt aatgtttatc
     2941 ggccgactgt tttcgtatcc gctcaccaaa cgcgtttttg cattaacatt gtatgtcggc
     3001 ggatgttcta tatctaattt gaataaataa acgataaccg cgttggtttt agagggcata
     3061 ataaaagaaa tattgttatc gtgttcgcca ttagggcagt ataaattgac gttcatgttg
     3121 gatattgttt cagttgcaag ttgacactgg cggcgacaag atcgtgaaca accaagtgac
     3181 ttaattaaca aaatgaacga cgaacttgtc aaacgatctc aatggctcct ggagaagctg
     3241 cgatacccct gggagatgat gcccctgatg tacgtgatac tgaaaggcgc cgacggagac
     3301 gtcaataaag cgcgccaacg gattgacgaa ggtatggggg ttcttaccgg ttgggactgt
     3361 ttccgaggta tcgatcgggt gtcactcact tcctgggtgc tcccattttg taactgctaa
     3421 cgcttattat tgagtttcag gacatctggg atcttcggtc gacggagtct attcccaaca
     3481 gtgccctgga tcaaacactg ccatcatgca gtttccgtag cctgttgggc tacgctcccc
     3541 gacttgacat cccccattct catcaaacaa caactcaagg cctgagacaa cgagtggtgg
     3601 aatttgcgca cgaagtcatt ggtttgtcct ggtaaaagtt aaaagggtta actggagggt
     3661 taattgacac ggtttcaact gatggcctta ttgacacacg gatgaaagac ttgcacgctt
     3721 gaccttctgt ctgtactaat aaaagttacg ttggctgggt tttggggtca taatggcccc
     3781 aaaatcgaat cgtcataact tcttgaaata caactcacgt ttaagaccat tcaagagtat
     3841 tagatcatcg tctataatag cagatttgaa atttacttca catttcggta ttgcagtgcc
     3901 ccttgcttcc acaatggaat tagttaaagt ttcgagagca ttgtcaatat caagtgttgt
     3961 tagcaaacaa atgctaacat caagattact atcgatgttt gattcacatg tattccaatc
     4021 agctcgtaaa acgtcaacat cccaccgcaa aatcgctagt gtttggaggg gattttaacc
     4081 tccaaattgc caaataacct ccaaatcatc acctccaagt tagttctaat acactccgtt
     4141 atatgaaata tggtggtgcg tcgatcgtcg caagtttatc gttaaacagt caataaaatg
     4201 agcattttat atcgtgatac atatgagaag atagaggttt caattaaaac aaatccacat
     4261 ggtgtcgcta ataaaattgt gcattttaag cgagttatat cctctgatca agataaaata
     4321 gaaaattcga tttttgaata ttcaattata agagcctgaa taactacaac atgtagtgaa
     4381 tcgaaactga tttatgacgg tttgtgaagg ttacacgtcc taagcatttg gattcaagaa
     4441 aagcaagaga tatgacgaat gtaaacttta tcgtatcaat gaagtaacta gcgtccagaa
     4501 cagtacaaac caacatcgta ccgtcgtatt ccactccggt cgttgcaata tctctaggtc
     4561 caccgaaaaa cactcatgac caagatcgtg tcgtcgatct tggtccaccg aaacaccgat
     4621 gtccatatcg tttcgtcgaa cttggaccaa cgattcatgc aactgatgac aacgcggccc
     4681 ccgggtcgta ccaatatccg aaaaatccaa ctgttcttct ctgcctcgca ggtcaagccg
     4741 tggtcaatga atactcacga ttgcacaatc tgaacatgtt cgacggtgta gagttgcgca
     4801 gtacgacgcg ccagtccgga tgatagactt tttacacgat cagcacgacc cactgcgctg
     4861 cggcaaaggt cgaaccgaaa caagaataaa ccacgaagat cagatcgatt cgacggaaga
     4921 agcaatcgaa tgcaaagaag aatcggaacg aagaaaactc taaagcatcg catatttaca
     4981 aagcataacg gaaaacccgc aagttcaaac tagtgattag tgtaagatga agcaaagcag
     5041 aaatgtagta tctagatttt tcaacgttag tttacaaaga taaaaaatga ggttggacat
     5101 acaatcgtgg gtattcgtct gagttcgtca caactgcacc ggaaactgtg aaacagaata
     5161 gagccaacct gtgcgcggag aatgttgagg tcattataag cttccttagc atccacgggt
     5221 gaaagtcgat cgacggaagc ctgcaagact ctgtcgatgg gctttcgtcc tagaagaata
     5281 agattaaacc tgaaatgtat tctcccgtgg aatggtttca tttgagtaat tctgtatctt
     5341 ctccttccca attccacgaa cgcgacgaac tctaatacaa acaacataat gaccacagtg
     5401 caaatgctgt ttaacgataa tagcgacatg cagccattct ggggctacca cgtggagctc
     5461 tacttgggag acagcgttcc taaagagtgt gaaagtgcaa acaagggagg aaaccaagag
     5521 tgcaaagcaa gtttagaggg aaaatttaaa aaatgcaaaa cagcagtagt acttaacttt
     5581 gaagattgtg tttcgaaagc cgaagtgtgt tccatctgcc accggaaaaa aacgacgaca
     5641 gcagaatcat caacaagcaa catccatccg aaaaaatccg ggaaaccgga tcttcaacca
     5701 accatcctac aatctacaaa ccagagatta tatctcttca atcgtttccg acatcggtcg
     5761 gtttcggtgc ccaaaatgat cggagaaaca cttatctctc tggagcttgc atgccattgc
     5821 gagcgtattt tggtagctgg ccgttgccaa acggctccgc aggtactgct attggaggtt
     5881 gtgcacgacc acgttgagtt tgccttttga gttggagagt gtgtcttttc gtcatatatt
     5941 cggccttttc aagggtgatt ttcaggctac gtaatgattg tatagtttaa ccagctaaaa
     6001 catattgatg acaagttcta tttcagcacc acaaacaagc ctgttaatgt ctctcaccgc
     6061 aaccattgtt ctgcgcgcgt tataatcagc atagaagttt attttctttg ggatgattca
     6121 aatattacgt gacgcaaagt ttgccaattt tagaacccct ccctcctcca cgtaacggct
     6181 tttgtgtgaa aaatttaaat tttgtgtata gaccgtagca tttcggaaga ccccctccct
     6241 tactctgttg agttacgtaa aatttcaacg atccttttgt agttctgaat tttatatcag
     6301 cgtgcagtgt tatgaagata tccacagtat aaaatattat tttattttaa attctatgct
     6361 gattatcaat gtgttactag tggcttttca tactcatgtt gcgagctcga tttggcgcac
     6421 ggggtcatct acacctgata cctttagggt cgttggggga ccacttagcg tgcacgtacg
     6481 gacattcaaa atgttgttca aatttttttc ttaccaagac gagcacttta caatgacaaa
     6541 cttataatat ttcgcatttt tgcgataaag tcaatggcat tatgaggcag agggaagcat
     6601 catcttcctt atgctatcca agtaatttgc cattgctttc aagaaatcga ggatttcatc
     6661 atgccaaatc agttatcttg tatcaaggca tgtatgtatg ttgtttgaag caactgtata
     6721 actgtttgaa actatctaat tggtgagctc gtttcattta gtatataata atgataattg
     6781 ctatggagac gttatttact agcaagtgat ttgacgacct gaaatcggaa caaatagaca
     6841 acgtttttat aaatacaata aatcagaact ttccattatt gggtacaaag agttgcgcta
     6901 tttcgatact gtcagatcag attttccagc acaacgatac cttgatatgc gataacttag
     6961 aattagacct tcaaatccat ctctccagct atgaacagtc atatagataa agccaatggc
     7021 gttatgaggt agcggaaagc gtcatctttc caatgctatc taagtacata atttgctata
     7081 gctttctatt aatcgtagtt tgagagatgc aaagtcagtt atctcgtatc aaggtttgat
     7141 tgttttggaa attagctaaa cagttgacat tatcacccgt ctttagggga taagcgcata
     7201 caaatgtgta tttagttgtt cattgaagta acgtaagata ggcaagtatg gaaacgagct
     7261 caccaaacgt cgaaatacgt ctaataaatt tgtgttcagc aggatggttc aaaatttatt
     7321 tgcatcacct caaaattaca gtacctagtg ctgtttgtga caaacatcaa aaggtaaaat
     7381 caaactcgtg gcgtcgtgca atctccatag aatgaacaat ttctaaccgt atttgatgga
     7441 aagacattga gtatactaac ctcttaacag cattacactt ttctataaac aataaataat
     7501 ttgttctatt ttacattttc tttccccact ttcgcccccc aataattcaa tccctcaaac
     7561 aggatacgac atttgttgca tctactttcc gaagcgttcc agcagacaca gacactggcc
     7621 ggacgaggag aacatctccg tcacccgcac tccgtctgcg tcacggtcgc catgtgccga
     7681 ttttcgtacc cggtcacagt ccagctcgcc ggatggtgcg ctcctccaag aacgtcatca
     7741 aggagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga
     7801 tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga
     7861 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     7921 ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg
     7981 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     8041 aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact
     8101 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     8161 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg
     8221 acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc
     8281 tgcccggcta ctactacgtg gactccaagc tggacatcac ctcccacaac gaggactaca
     8341 ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagtaatggt
     8401 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     8461 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     8521 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     8581 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     8641 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     8701 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     8761 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     8821 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     8881 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     8941 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     9001 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     9061 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
     9121 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     9181 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     9241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaaa
     9361 gcttgcggcc gcaattgatc ggctaaatgg tatggcaaga aaaggtatgc aatataataa
     9421 tcttttattg ggtatgcaac gaaaatttgt ttcgtcaacg tatgcaatat tttttattaa
     9481 aagagggtat gcaatgtatt ttattaaaaa cgggtatgca atataataat cttttattgg
     9541 gtatgcaacg aaaatttgtt tcgtcaaagt atgcaatatt ttttattaaa agagggtatg
     9601 caatgtattt tattaaaaac gggtatgcaa taaaaaatta tttggtttct ctaaaaagta
     9661 tgcagcactt attttttgat aaggtatgca acaaaatttt actttgccga aaatatgcaa
     9721 tgtttttgcg aataaattca acgcacactt attacgtgac ctgcaggcaa acaaacgcga
     9781 gataccggaa gtactgaaaa acagtcgctc caggccagtg ggaacatcga tgttttgttt
     9841 tgacggaccc cttactctcg tctcatataa accgaagcca gctaagatgg tatacttatt
     9901 atcatcttgt gatgaggatg cttctatcaa cgaaagtttt gttttttttt aataaataaa
     9961 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
    10021 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
    10081 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
    10141 aatctactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    10201 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    10261 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    10321 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    10381 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    10441 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10501 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10561 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10621 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10681 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    10741 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10801 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10861 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10921 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10981 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    11041 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    11101 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    11161 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    11221 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    11281 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    11341 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    11401 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    11461 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    11521 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    11581 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    11641 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    11701 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    11761 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    11821 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    11881 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
