LOCUS       sequence-39524-e        4406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S2T1L
FEATURES             Location/Qualifiers
     source          1..4406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1507..1525)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1521..1568
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1636..1654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1637..1654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1945..1964)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2175..2194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2294..2316
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2354..2372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2440..2544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2545..2613,2614..3405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2763..2782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3576..4164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4065..4084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4318..4335
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggtaatacga ctcactatag ctattcgctg gtcagttatg gctcgcgctg
       61 cgttcctatt caagactgtt ggatttggtg gtctgcaaaa tgtgccaatt aatgatgagt
      121 tatcgtcaca tctacttcga gccggtaatt cgccatggca gctgactcag ttcttagatt
      181 ggataagtct tggaagagga ttagctacat cagctcttgt tccaaccgct ggttcaagat
      241 attatcagat gagttgttta ttgagtggta ccctccagat tccatttcgt cctaatcatc
      301 gatggggaga tattaggttt ctgcgtctag tgtggtcagc tcctacgctt gacgggttgg
      361 ttgttgcccc accgcaggtc ttagctcagc cagcgttaca ggcgcaggca gatcgagtgt
      421 atgattgtga tgattaccca ttcttagcac gtgacccgag atttaagcat cgagtgtatc
      481 aacaattgag cgccgtaact ctgcttaact tgacggggtt tggtccaatt tcctatgttc
      541 gagtagacga ggatatgtgg agtggggatg tgaaccagct tctcatgaac tacttcgggc
      601 atacgtttgc ggaaattgcg tatacattat gtcaagcttc agccaataga ccttgggagc
      661 atgatggtac gtatgcgagg atgactcaaa ttatactgtc cttattttgg ttatcatatg
      721 tcggtgtaat tcatcagcag aacacgtacc ggacgttcta tttccaatgc aatcggcgcg
      781 gtgatgctgc tgaagtatgg attctttcct gctcattaaa ccactccgcc cagattagac
      841 cgggtaatcg cagtctattc gtcatgccaa caagtccaga ttggaatatg gacgtcaatt
      901 tgattttaag ttcgacgttg acagggtgct tgtgttcggg ctctcagtta ccgcttattg
      961 acaataactc agtgcctgcg gtttcgcgta acatccatgg ttggactggc agagctggca
     1021 accagctgca tggtttccaa gtgcgacgaa tggtgactga attctgtgac agattaagac
     1081 gcgatggagt tatgactcaa gctcagcaaa atcaaattga agcgttggca gatcaaactc
     1141 aacagtttaa gagggataaa cttgaggcgt gggctaggga agatgatcag tataatcagg
     1201 ctaatccgaa ttctacgatg ttccgtacga agccatttac gaatgcgcaa tggggacgag
     1261 gaaataccgg agcgactagt gccgcaattg cagcccttat ctaatcgtct tggagtgagg
     1321 ggatcccccc acacccctca cgactgacca cacattcatc gggtcggcat ggcatctcca
     1381 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgtc gtccactcgg atggctaagg
     1441 gagagctcaa aaaaaaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1501 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1561 gttttttgct gaaaggagga actatatccg gatcgagatc ctctagagtc gacctgcagg
     1621 catgcaagct tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     1681 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     1741 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     1801 acgaaccttc tgagtttctg gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa
     1861 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     1921 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1981 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     2041 ccgggggact gttgggcgcc atctccttgc accattcctt gcggcggcgg tgctcaacgg
     2101 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata
     2161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2281 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2341 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     2401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3061 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3721 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4321 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4381 tggccgattc attaatgcag ggggat
//
