LOCUS       sequence-39525-e        4274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S3T1L
FEATURES             Location/Qualifiers
     source          1..4274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1375..1393)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1389..1436
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1504..1522)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1505..1522)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1813..1832)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2043..2062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2162..2184
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2222..2240)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2308..2412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2413..2481,2482..3273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2631..2650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3444..4032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3933..3952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4186..4203
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atcgatcccg ggttaatacg actcactata gctaaagtca cgcctgttgt cgtcactatg
       61 gcttcctcac tcagggctgc gatctctaag atcaagagag atgatgttgg tcagcaagtt
      121 tgtccaaatt atgtcatgct tagatcatcg gtcacaacga aggtggtacg aaacgttgtt
      181 gagtatcaaa tccgtacagg tggattcttt tcgtgcctag ctatgttgag accgctccag
      241 tatgctaaac gtgagcgtct gcttggacaa aggaatctgg aacgcatatc gactagggac
      301 attcttcaga cacgcgattt gcactcattg tgcatgccaa ctcctgacgc gccaatgtcc
      361 aatcatcagg cagccaccat gagagagctg atctgcagct acttcaaggt cgaccacact
      421 gatgggttga aatatatacc catggatgag agatattctc catcatcgct tgccagattg
      481 ttcactatgg gtatggctgg actacacatt accactgagc cttcctacaa acgtgtgccc
      541 atcatgcact tggcagcaga tttggactgc atgacgttag ctttacccta catgattaca
      601 cttgatggtg acacagtggt acctgtcgcc ccaacgcttt ctgcagaaca gcttttggat
      661 gatggactta agggattagc atgcatggat atctcatacg gatgtgaggt ggacgctagc
      721 aaccgatcag ctggtgatca gagcatggat tcttcacgat gcatcaatga gttatattgc
      781 gaggaaacgg cagaagctat ctgtgtactc aaaacatgtc ttgtgctgaa ctgtatgcaa
      841 ttcaaacttg agatggatga tttagcacac aacgctactg agctggacaa gatacagatg
      901 atgatacctt ttagtgaacg tgttttcaga atggcttctt catttgctac cattgatgcc
      961 cagtgtttca ggttttgtgt gatgatgaag gataagaatt tgaagataga catgcgtgaa
     1021 acgatgagac tttggactcg atcggcgttg gacgattcag tggttacgtc atctttgagt
     1081 atttcgctgg atcgaggtcg atgggtagca gctgatgcta ctgatgctag attgctggtg
     1141 tttccaattc gcgtgtaatg ggtgagtgag ccgatgtggt cgccaagaca tgtgccggtg
     1201 tcttggtggt gggtggcgcc taatcatcgg gtcggcatgg catctccacc tcctcgcggt
     1261 ccgacctggg catccgaagg aggacgtcgt ccactcggat ggctaaggga gagctcaaaa
     1321 aaaaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1381 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     1441 aaggaggaac tatatccgga tcgagatcct ctagagtcga cctgcaggca tgcaagcttg
     1501 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     1561 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     1621 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     1681 agtttctggt aacgccgttc cgcaccccgg aaatggtcag cgaaccaatc agcagggtca
     1741 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     1801 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     1861 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     1921 tgggcgccat ctccttgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     1981 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgatatg gtgcactctc
     2041 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     2221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4081 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4141 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4201 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4261 taatgcaggg ggat
//
