LOCUS       sequence-39526-e        7218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pBacT7-S1T3D
FEATURES             Location/Qualifiers
     source          1..7218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1174..1191
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     terminator      complement(1317..1364)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1360..1378
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     misc_recomb     3017..4374
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(3033..4374)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4521..4976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4608..4627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4818..4839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4993..5012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5112..5134
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5172..5190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5258..5362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5363..5431,5432..6223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5581..5600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6394..6982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6883..6902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7136..7153
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg
     1261 caggcctcga gttcgaatct agaggatctc gatccggata tagttcctcc tttcagcaaa
     1321 aaacccctca agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg
     1381 gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcctt ttttttgagc
     1441 tctcccttag ccatccgagt ggacgacgtc ctccttcgga tgcccaggtc ggaccgcgag
     1501 gaggtggaga tgccatgccg acccgatgaa atgccccagt gccgcggggt ggtctgatcc
     1561 tcacgtgaaa ctacgcgggt acgaaacggt catggcaggc cacttactgt ttgagtgcgt
     1621 aattgagcca cccccctcaa cacgtaaccg aagtactcca tcctgccact gctccaccca
     1681 cagattctta ctcatatact gaggcgtacc acccgacgac aaccctatag ctacagtctg
     1741 tgctccatat gtgaccacgt cattatgaaa agcgggctca gtgtctcctg taagtaacgg
     1801 tggtaacaat ccggtaacaa agttcaacga tagatctcca ccgtcagcta tagagaaacc
     1861 gtcaaaagct ggaagacata tatgtatgta atcatctaca ataaaaatgt cggagttcac
     1921 ctgtaccctc caattcagcc cactaccaga ataggagaca attcctatcc acatgctctg
     1981 cctaaaccta taatttggac tcattccgat accgccgcta acatcagcta tcggatacct
     2041 caaattcggg gatgtcgatc taacagttag ctgtccacta gaattaattt caagtgttga
     2101 actgtctatt agcatatcca gcaccttcgt gctactgttt aatctcaagg gagtcactgc
     2161 cgacgccacg taactttgct cagttgcgcc tatccttgag ttgatagaat caaacacagt
     2221 cgtcttgaga gtcaagttat tattaactat ctggaattga tcagtattaa atcgaaactg
     2281 aagtccacca ttttgaatat tcagacccgt atttcctggc aatcggatgg cgagattatt
     2341 ccctgacaac gtgagtccat catttaatcc catggtcata cggttattac ggattgagag
     2401 gggagctccc gcgctagtga ccgccgtgcg ctctaatgtg gatatcctag attcgaaatc
     2461 cgcttgtatg gatgttactc gtaacgtcag agttgatagc tcggtggtca atgatccaat
     2521 gttacgttct gcagtatcca ctctcgcaac gagattgtcg tgatcaacgc gtagctctgc
     2581 aagtcctgtc tcaagttgtc ccactcgagc acccaactgg gtaacactcg aatcaagtcc
     2641 attgacaact gtttggaggg ctccgataga gctttccaat ctggagattg caagttgagc
     2701 atcactgact gatgcaacca agtcatcccg actttgctca agagcgatga ttcgtttgtt
     2761 tgcatcatcg agtccctggg tgatccggag gatagtatca gagtgtattt gagacgtctt
     2821 ctcgagcgcc gagacccttg attcaagccc ttttgacagt gatactccat tatcactcgt
     2881 taatgcgatt atcagccgta ctacttcttc acgtaggcga ggatccatcc gaccaatagc
     2941 tatagtgagt cgtattatct agaagatctg gtaccgagct cgaattcccg ggcggccgct
     3001 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
     3061 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
     3121 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
     3181 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
     3241 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
     3301 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
     3361 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
     3421 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
     3481 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
     3541 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
     3601 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
     3661 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
     3721 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
     3781 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
     3841 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
     3901 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
     3961 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
     4021 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
     4081 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
     4141 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
     4201 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
     4261 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
     4321 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
     4381 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
     4441 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4501 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4861 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4921 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     4981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5041 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5101 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5161 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5221 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5581 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5821 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5881 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5941 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6001 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6061 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6121 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6181 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6541 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7021 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7081 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7141 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7201 gccgattcat taatgcag
//
