LOCUS       sequence-39528-e        7265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pBacT7-S1T1L
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     327..1154
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             327..451
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(489..1094)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        1158..1249
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1174..1191
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     primer_bind     complement(2910..2928)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2924..2971
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     3064..4421
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(3080..4421)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4568..5023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4655..4674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4865..4886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5040..5059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5159..5181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5219..5237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5305..5409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5410..5478,5479..6270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5628..5647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6441..7029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6930..6949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7183..7200
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
       61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
      121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
      181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
      241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
      301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
      361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
      421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
      481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
      541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
      601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
      661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
      721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
      781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
      841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
      901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
      961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
     1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg
     1261 caggcctcga gttcgaatct agataatacg actcactata gctattcgcg cctatggatg
     1321 catctctcat tacagagata cggaaaatag tactccaact atctgtatca agcaatggct
     1381 cccagtcaaa agaaatcgag gaaatcaaga aacaagtcca ggtcaacgtt gatgatatca
     1441 gggctgccaa tattaaactc gacggacttg gaagacagat tgctgacatc agcaatagca
     1501 tctcaaccat tgagtcaaga ttgggtgaga tggataatcg acttgtgggt atctcgagtc
     1561 aggtcacgca attatctaac tcagttagcc agaacactca gagcatatcc tcattgggtg
     1621 acagaatcaa tgctgtcgaa ccacgagttg acagtctgga tacggtcacg tctaatctca
     1681 ctggacgaac atccactttg gaggcagatg ttggaagctt acggacagaa ctagcagcgc
     1741 taacaacacg ggtgacaact gaggttacaa ggttagatgg tctaatcaat agtggccaga
     1801 attcgattgg tgagctatcc acaagactat ccaatgtgga gacgtctatg gtgacgacgg
     1861 ctggacgggg actgcagaaa aacggaaaca ccttgaacgt cattgtaggt aatggaatgt
     1921 ggtttaatag ttctaatcaa ttgcagctcg acctttcggg gcaatcaaaa ggggtgggat
     1981 ttgtcggcac aggaatggtg gttaagattg atactaatta ttttgcttac aatagtaatg
     2041 gagagattac attggtgagt caaatcaatg aattgccatc gcgcgtatca acactggaat
     2101 cagcgaaaat cgattcagtt ttacctccat taaccgtacg cgaagcgagc ggcgtacgta
     2161 ccctgagctt tggttatgat acgagcgatt ttacaatcat caactccgta ctgtcgttac
     2221 ggtcacgttt gactcttccg acatacaggt accctctgga gctcgacaca gcaaataata
     2281 gagtgcaggt ggcagatcgt tttggcatgc gcacgggtac ttggacggga caattgcaat
     2341 atcagcaccc acaattgagt tggagagcaa atgtcacttt gaatttgatg aaggtggatg
     2401 attggttggt gttgagcttt tctcagatga cgactaactc aataatggca gatgggaaat
     2461 ttctgattaa ttttgtgtct gggttatctt ctggatggca gacgggggat actgaaccat
     2521 cgtcaactat tgatccattg tctacgacat ttgccgcggt ccaatttcta aataacggtc
     2581 aacgcattga tgcgtttagg atcatgggag tatcggaatg gacggatgga gaattagaga
     2641 ttaagaatta tggtggcaca tacaccggtc atactcaagt atattgggct ccgtggacga
     2701 tcatgtatcc atgcaatgtg aggtgaatct agcgcgaacc ctcggcacaa ggggtcaatc
     2761 atcgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
     2821 gtcgtccact cggatggcta agggagagct caaaaaaaag gatccggctg ctaacaaagc
     2881 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     2941 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatcgag
     3001 atcctctaga agatctggta ccgagctcga attcccgggc ggccgcttaa ttaattgatc
     3061 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt
     3121 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga
     3181 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt
     3241 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac
     3301 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag
     3361 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat
     3421 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca
     3481 cgttaaataa agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
     3541 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
     3601 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
     3661 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
     3721 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
     3781 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
     3841 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
     3901 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
     3961 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
     4021 acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag
     4081 cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca
     4141 cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc
     4201 gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc
     4261 gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat
     4321 ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa
     4381 tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata
     4441 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgcct
     4501 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4561 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4621 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4681 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4741 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4801 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4861 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     4921 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4981 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     5041 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5101 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5161 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7261 tgcag
//
