LOCUS       sequence-395301-        2465 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of the CrGLK coding sequence from C. roseus in a 
            plant binary vector for Agrobacterium-mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pLC047 - pL2_p2x35s-CrGLK-tMAS
FEATURES             Location/Qualifiers
     source          1..2465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        405..750
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     693..713
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      2213..2465
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
       61 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      121 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      181 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      241 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      301 acgtctacaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctggtctac
      361 tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa
      421 aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      481 aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct
      541 atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc
      661 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
      721 taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac
      781 aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat tatcgataca
      841 atgcttgctg tgtcaccatt gaggagcaat aataatgaga gaaaaaagga gatatgtgaa
      901 attagtagta gtactagttt tccagttgtt gatgattttc ctgattttgg gggagcaaat
      961 ttgcttgata gcattgattt tgatgaactt tttgttggga ttaacggtga ggatgatgtt
     1021 ttaccagatt tggaaatgga tcctcatgat attcttgctg aattctcact tactgaatca
     1081 ttatcaccat ctgtttctct tgatcatcaa catcatgaac attctcaaat tacctcctca
     1141 atttcggcgg ctgattatga taaactagta gaattagagg ataaggctaa ttcatcatcc
     1201 gcttcagacg acgtcggttc aggctcatct actttaactc aaggagaaga tgttgttagc
     1261 aaatcaacgg ttttaaatcc atcttctaaa gaaggtgata aaggaaggaa gtcatcttct
     1321 actcaatcaa agaattcttc tgggaagaag aaagtgaagg tggattggac accagagctg
     1381 cacagaagat ttgtacaagc agtggagcag ctaggtgtgg ataaggcagt cccttcaaga
     1441 attcttgaac ttatgggaat tgattgtctt actcgccaca acattgctag ccatcttcaa
     1501 aaatacagat cccacaggaa acatttgcta gcaagagaag ctgaggcagc aaattggagc
     1561 cagaggcggc aattatacgg aggcactgca gcagcagcag gaggagggaa gagagatgta
     1621 aatccatggc ttgctccaac catgggattt cctcctccca tggcaccggc ggccgtccct
     1681 cattttagac ccttacatgt atggggtcac ccatctgtcg accaatcttt aatgcacgtt
     1741 tggcctaaac atttagctcc tccgccccga ccaccaacat ggccaccaac tccaacacca
     1801 acaccaacac caccaccacc accgccagat cctaattcct tttggcattc acctcaccaa
     1861 cgtgtaccaa accctcttac cccaggaacg ccttgctttc cgccaccttt aacaccagcg
     1921 agatttccaa caccgccggt ccccggaata ccaccacctc caatgtacaa agtagacccg
     1981 gtcgcaggca tgggtgtgcc ggcacctgct accggacaag cagtacccca tgcacctcct
     2041 cttgactttc acccctcgac ggagagcgta gatgcagcta tcggagatgt tttatcgaaa
     2101 ccctggctgc cacttcctct tgggctgaaa ccgccgtcga ttgacagtgt actggtggag
     2161 ctacagcggc aaggaatacc gaaaattcca ccagtttgta cgggttcgta agcttggact
     2221 cccatgttgg caaaggcaac caaacaaaca atgaatgatc cgctcctgca tatggggcgg
     2281 tttgagtatt tcaactgcca tttgggctga attgtagaca tgctcctgtc agaaattccg
     2341 tgatcttact caatattcag taatctcggc caatatccta aatgtgcgtg gctttatctg
     2401 tctttgtatt gtttcatcaa ttcatgtaac gtttgctttt cttatgaatt ttcaaataaa
     2461 ttatc
//
