LOCUS       sequence-395308-        5062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains EFR promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP10
FEATURES             Location/Qualifiers
     source          1..5062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4648..4667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4649..4667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4677..4699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4893..4912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5012..5034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tatctagacg attaagtaat tgagcatgta
     2281 aaagcagaaa agacaaacaa ggtttctctc aaggatacaa cttttatata aactctccct
     2341 cacgttcgat ggagttcatt ttcttaacgg ttgcgacttg agagattctt tttcttatac
     2401 agtttcgtta ttagtgtata ttaattattt ttaagtgcat tacttaatta atctatttca
     2461 atagagaatt tcccacttgg cctctaaact tacaaagtct ttatctaatt ggtcactgtc
     2521 tcaagcgaaa ccgttgatta tttctcttta agtgaatgaa atcattgacc ttaggtcagt
     2581 aggaaaaata tatccgcaag ttgatgatta tacaattaat taacatttcc aaaaatctct
     2641 gatctgtctt tgatccgtag tgaaccgtaa agatttaacc ttcattccac tcattggtga
     2701 acaaatcaaa tacttttgtt gaaccgatga ttattctcat agttactttc taatatcagt
     2761 ttatttgtaa aattacatac ttttgaacat caattggttt gtgggttaaa caattgatgg
     2821 aaaacaatga ggatgcttgg acagagttaa gtcaagttgg gaagatccaa aaaaagagtg
     2881 gaaagttaag gaactgtagt actcaaacaa aaaactaagt ggactaatga tgaaaagaga
     2941 ctctaaaatt catttccaaa gctgtaggtg ttcaatgttc acctcatgga aaacataatg
     3001 atctaaggga cggatttgac tgagtaaata ccggacagag ttctcttgtt agtttttcta
     3061 aggtcatttc tgcttttcac gaatgacctg aacagagagc caaaacaatg taagcttata
     3121 agtattggga acataaaaag attggcatag catcaactaa gaatcatacc agaaaagcaa
     3181 cagcctgcct gatatggtcc aacttatccc aagctaagcc agcatactgt aagagattta
     3241 aaccgtaagg tcattttttc gggtgaaaat gtcgaatagg caaaggcgtg tgacgagaac
     3301 ttgtggctta cttcatcggt agcttctaca caccattttt tttagttcag cctgacctgc
     3361 tttaacgtgg tccccatttc ttggtgtgca agattcacga cattgaagaa aactgcacaa
     3421 gtttattaca gagaaatctt tacgtcaaaa ttggattgag agatactaaa ataagtaatt
     3481 actagtgagc atgcctgttg agaagatgaa gattgatgaa ggcaaatatt tgtgtgaata
     3541 cttttctgac taagaatggt ggaacctgca tcatgtaaac atagaatata atcacaattt
     3601 ttattttgag aagccattag aattgaacaa tgcatgggtt tttgtttatt caaagatggg
     3661 attttattaa gattgactat atcatacaaa ggcttaaaca aaagccctaa ctttagattt
     3721 acaaaagccc aagaaaggcc catcaaataa ctaacttttg cagaagcaaa tctatctgaa
     3781 cttcatccat ggatacgttt ccaacaatac atgggcttgt cacttataat cgtggcttgt
     3841 gtcttcaaat tataaggtca cataacaaaa ctacatgcag tctattgttt ttctccttcg
     3901 tcgacgaact acttttgccg tttaatagat cataaacacc acacatttct accgtcacag
     3961 tgacaacact tcccaattat tctgattatg gcgcagggga ttcgccggaa gatccaatct
     4021 gtgctgattt tgcaagaatc gatgggcttc ctcaaattat cgggcgcttt ttacgacact
     4081 gcaattgatc gaaaccttcg caaaaaatcg taaagtaaaa atcctctttt tatttttatt
     4141 taagaccctt tagtagttta tcaacaagta aagattgcat tttttttggt acatgtaatt
     4201 tgtttcagaa tttataaaca agaatttcta tcaatgtctt tgaattaact ttgataaatt
     4261 caaactttct gatggatggt tgtcaataca agtttcagaa gaagattgaa accaaaatga
     4321 ctagacaaga cgtttggtgt atgtcgacca atgtgttctg ttttgggtag cactttgcct
     4381 taactctgtt tacacacaca cgtttatgtg ttttgactgt tgaaaaaaaa acgaaagata
     4441 tttcaaaagt ctacgcagta cacaatttgt ttgtttgtga ccatttgcaa tgattttgga
     4501 ccgcatttaa ttgccccacg actgtaatgg ggatcacagt ttggattttg cttcttgttt
     4561 accttaaact tcctttcatt ttaatgttga agcagaagaa cctttctttt atctttttat
     4621 aatcgacaac atgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt
     4681 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4741 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4801 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4861 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4921 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4981 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5041 ccgtcatcac cgaaacgcgc ga
//
