LOCUS       sequence-395324-       11776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid could be used to clone gene(protein) of interest in 
            place of Citrine to use in proximity labeling approach in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Zhang Y, Song G, Lal NK, Nagalakshmi U, Li Y, Zheng W, Huang PJ, 
            Branon TC, Ting AY, Walley JW, Dinesh-Kumar SP
  TITLE     TurboID-based proximity labeling reveals that UBR7 is a regulator of
            N NLR immune receptor-mediated immunity.
  JOURNAL   Nat Commun. 2019 Jul 19;10(1):3252. doi: 10.1038/s41467-019-11202-z.
  PUBMED    31324801
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-19"; volume: "10(1):3252. doi"; pages: " 
            10.1038/s41467-019-11202-z"
COMMENT     Sequence Label: pUBQ:Citrine-TurboID-3xMyc
FEATURES             Location/Qualifiers
     source          1..11776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             290..1084
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1913..1930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1945..2085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2011..2030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2171..2193
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2429..2623
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2689..3762)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4191..4820)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6121..6145
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6333..6355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6347..6364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6348..6364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(7701..7703,7704..8414)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8004..8023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8351..8372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8421..9377
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             9384..9413
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9420..9449
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9456..9485
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      9512..9764
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9781..9797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9781..9797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9794..9816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9805..9821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9829..9835,9836..9853,9854..9859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9874..9895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10086..10762
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10699..10719
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10807..11358
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11365..11539
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11617..11641
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
       61 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
      121 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
      181 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
      241 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
      301 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
      361 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
      421 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
      481 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
      541 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
      601 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
      661 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
      721 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
      781 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
      841 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
      901 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
      961 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     1021 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     1081 ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1861 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1921 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     1981 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2041 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     2101 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2161 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2221 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     2281 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     2341 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     2401 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     2461 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     2521 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     2581 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     2641 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     2701 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     2761 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     2821 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     2881 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     2941 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     3001 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     3061 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     3121 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     3181 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     3241 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     3301 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     3361 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     3421 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     3481 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     3541 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     3601 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     3661 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     3721 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     3781 ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg
     3841 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     3901 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     3961 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     4021 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     4081 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     4141 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     4201 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     4261 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     4321 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     4381 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     4441 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     4501 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     4561 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     4621 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     4681 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     4741 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     4801 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     4861 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     4921 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     4981 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
     5041 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     5101 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
     5161 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
     5221 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
     5281 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
     5341 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
     5401 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
     5461 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
     5521 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
     5581 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
     5641 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
     5701 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
     5761 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
     5821 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
     5881 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
     5941 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
     6001 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     6061 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     6121 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     6181 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
     6241 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6301 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     6361 cagtgccaag cttgcatgcc tgcagatcag gatattcttg tttaagatgt tgaactctat
     6421 ggaggtttgt atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt
     6481 attcaaagaa tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt
     6541 ggtcattgga ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat
     6601 atatgaatta aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga
     6661 cttgttcacc aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa
     6721 tatccaataa cactaaaaaa ttaaaagaaa tggataattt cacaatatgt tatacgataa
     6781 agaagttact tttccaagaa attcactgat tttataagcc cacttgcatt agataaatgg
     6841 caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac
     6901 gcttcaatgc agtgggaccc acggttcaat tattgccaat tttcagctcc accgtatatt
     6961 taaaaaataa aacgataatg ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg
     7021 gctggatctt atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg
     7081 tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt
     7141 ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca
     7201 atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag
     7261 tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt
     7321 cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt
     7381 gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg
     7441 atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt
     7501 ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat aaatatcatc
     7561 cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat
     7621 ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt
     7681 ctgattaaca gatgaggcct gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     7741 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     7801 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     7861 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
     7921 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     7981 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     8041 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     8101 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     8161 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     8221 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     8281 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     8341 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     8401 acgagctgta caagcccggg aaagacaata ctgtgcctct gaagctgatc gctctcctgg
     8461 ctaatggcga gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccagggccg
     8521 ctatcaacaa gcacattcag actctgcgcg actggggcgt ggacgtgttc accgtgcccg
     8581 gaaagggcta ctctctgccc gagcctatcc cgctgctgaa cgctaaacag attctgggac
     8641 agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc
     8701 tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag
     8761 ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta
     8821 tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg
     8881 tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg
     8941 acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag
     9001 gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg
     9061 aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga
     9121 atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg
     9181 aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg
     9241 tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg
     9301 gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc
     9361 tgcggtctgc cgaaaagcct agggaacaaa agttgatttc tgaagaagat ttgaacggtg
     9421 aacaaaagct aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag
     9481 acttgaacgg atgagctctg agaatttccc cgatcgttca aacatttggc aataaagttt
     9541 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     9601 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     9661 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     9721 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcgtaatcat
     9781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9961 cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg
    10021 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
    10081 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
    10141 gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat
    10201 cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat
    10261 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
    10321 caagtggatt gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa
    10381 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
    10441 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
    10501 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
    10561 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    10621 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
    10681 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
    10741 tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag
    10801 tctaccatga gcccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg
    10861 ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc
    10921 gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc
    10981 tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg cccctggaag
    11041 gcacgcaacg cctacgactg gacggccgag tcgaccgtgt acgtctcccc ccgccaccag
    11101 cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc
    11161 ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag
    11221 gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg
    11281 catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg tccggtcctg
    11341 cccgtcaccg agatttgact cgagtttctc cataataatg tgtgagtagt tcccagataa
    11401 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
    11461 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
    11521 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt
    11581 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
    11641 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
    11701 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
    11761 catgttaccg atgcta
//
