LOCUS       sequence-395325-       11515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid could be used to clone gene(protein) of interest in 
            place of Citrine to use in proximity labeling approach in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Zhang Y, Song G, Lal NK, Nagalakshmi U, Li Y, Zheng W, Huang PJ, 
            Branon TC, Ting AY, Walley JW, Dinesh-Kumar SP
  TITLE     TurboID-based proximity labeling reveals that UBR7 is a regulator of
            N NLR immune receptor-mediated immunity.
  JOURNAL   Nat Commun. 2019 Jul 19;10(1):3252. doi: 10.1038/s41467-019-11202-z.
  PUBMED    31324801
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-19"; volume: "10(1):3252. doi"; pages: " 
            10.1038/s41467-019-11202-z"
COMMENT     Sequence Label: p35S:Citrine-TurboID-3xHA
FEATURES             Location/Qualifiers
     source          1..11515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..35
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     27..44
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        771..817
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(826..828,829..831,832..1542)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1132..1151)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1479..1500
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1549..2505
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2512..2592
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      2613..2865
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2886..2902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2886..2902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2899..2921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2910..2926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2934..2940,2941..2958,2959..2964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2979..3000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3191..3867
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3930..4727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3987..4006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4597..4616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4784..4958
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5036..5060
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5485..6279
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6366..6954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6855..6874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7108..7125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7140..7280
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7206..7225)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7366..7388
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7624..7818
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7884..8957)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9386..10015)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11316..11340
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatggtca acatggtgga gcacgacact
      421 ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc tattgagact
      481 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      541 ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa
      601 ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg acccccaccc
      661 acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      721 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      781 tcctctatat aaggaagttc atttcatttg gagaggattg gtaccatggt gagcaagggc
      841 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      901 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      961 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1021 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1081 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1141 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1201 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1261 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1321 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1381 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1441 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1501 accgccgccg ggatcactct cggcatggac gagctgtaca agcccgggaa agacaatact
     1561 gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg
     1621 ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac
     1681 tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg
     1741 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
     1801 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
     1861 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
     1921 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
     1981 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
     2041 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
     2101 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
     2161 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     2221 caggaagcag ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg
     2281 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag
     2341 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     2401 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     2461 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagcctag gtacccatat
     2521 gacgttccag attacgctta tccgtacgat gtgcccgact atgcgtaccc ttatgacgta
     2581 ccagattacg cctgagctct gagaatttcc ccgatcgttc aaacatttgg caataaagtt
     2641 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2701 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2761 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2821 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgtcga cgaattcgta
     2881 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2941 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3001 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3061 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgccaaca
     3121 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     3181 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     3241 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     3301 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     3361 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     3421 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat
     3481 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     3541 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     3601 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     3661 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     3721 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     3781 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     3841 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
     3901 tcgagctttc gcagatctgt cgatcgacca tggggattga acaagatgga ttgcacgcag
     3961 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4021 gctgctctga tgccgccgtg tttcggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4081 agaccgacct gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc
     4141 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4201 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4261 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4321 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4381 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4441 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acacatggcg
     4501 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4561 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4621 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4681 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4741 gttcggatcg atcctctagc tagagtcgat cgacaagctc gagtttctcc ataataatgt
     4801 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
     4861 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     4921 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa
     4981 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     5041 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     5101 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     5161 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     5221 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     5281 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
     5341 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
     5401 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     5461 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     5521 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     5581 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     5641 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     5701 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     5761 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     5821 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     5881 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     5941 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     6001 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     6061 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     6121 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     6181 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     6241 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa
     6301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6481 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7141 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     7201 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     7261 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7321 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7381 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     7441 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     7501 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     7561 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     7621 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     7681 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     7741 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     7801 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     7861 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     7921 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     7981 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     8041 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     8101 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     8161 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     8221 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     8281 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     8341 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
     8401 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
     8461 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
     8521 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
     8581 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
     8641 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
     8701 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
     8761 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
     8821 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
     8881 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
     8941 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
     9001 ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
     9061 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
     9121 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
     9181 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
     9241 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
     9301 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
     9361 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
     9421 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
     9481 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
     9541 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
     9601 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
     9661 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
     9721 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
     9781 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
     9841 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
     9901 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
     9961 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    10021 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    10081 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    10141 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    10201 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    10261 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    10321 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    10381 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    10441 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    10501 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    10561 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    10621 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    10681 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    10741 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    10801 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    10861 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    10921 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    10981 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    11041 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    11101 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    11161 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    11221 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
    11281 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    11341 aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc
    11401 tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    11461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaa
//
