LOCUS       sequence-395330-       10930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs 
            targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting 
            EEA-motif (Addgene #102898). Includes PGK-puro selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Sokka J, Yoshihara M, Kvist J, Laiho L, Warren A, Stadelmann C, 
            Jouhilahti EM, Kilpinen H, Balboa D, Katayama S, Kyttala A, Kere J, 
            Otonkoski T, Weltner J, Trokovic R
  TITLE     CRISPR activation enables high-fidelity reprogramming into human 
            pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2022 Feb 8;17(2):413-426. doi: 
            10.1016/j.stemcr.2021.12.017. Epub 2022 Jan 20.
  PUBMED    35063129
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.12.017"; journalName: "Stem Cell Reports"; 
            date: "2022-02-8- 8"; volume: "17"; issue: "2"; pages: "413-426"
COMMENT     Sequence Label: GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..484
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        506..581
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     613..629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..878
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        900..975
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(987..1003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1007..1023
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1024..1272
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1294..1369
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1400..1416
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1417..1665
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1681..1756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1788..1804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1805..2053
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2069..2144
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2302..2335
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2396..2895
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2431..2454)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2828..2847
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2935..3534
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2935..2954)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             3983..5908
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGXGXGGGAGXGGGAGGAGXGGGAGGAGGAGAGGGAGAGGAGGAGGXGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGXGAGGXGGXGXGGXGGXGXGGXGGXGXGGGXGGXGXG
                     GGXGGXGXGGXGGXGXGGXGGXGXGGXGGXGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6076..6109
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      6296..8085
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8654..8673)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8773..8795
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8833..8851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8919..9023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9024..9092,9093..9884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9242..9261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10055..10643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10544..10563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctctaatacg actcactata gggagacaag cttgcatgcc tgcaggtcga
      181 ctctagagga tctcgagctc aagcttcgaa ttccccctgt aaaacgacgg ccagtgaggg
      241 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      301 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      361 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      421 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      481 caccgatctc agagaatcat tacaagtttt agagctagaa atagcaagtt aaaataaggc
      541 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc
      601 tgtttcctca tggtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt
      661 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
      721 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      781 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      841 tcttggcttt atatatcttg tggaaaggac gaaacaccga gggaatgtat gatcctgggg
      901 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      961 gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc ctggacgtaa aacgacggcc
     1021 agtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     1081 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     1141 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     1201 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     1261 ggacgaaaca ccgtttaaga ggaagatatc ttggttttag agctagaaat agcaagttaa
     1321 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctgg
     1381 tcatagctgt ttcctccagg taaaacgacg gccagtgagt gcctatttcc catgattcct
     1441 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     1501 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1561 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1621 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgccca gcactttggg
     1681 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1741 ggcaccgagt cggtgctttt tttcatggtc atagctgttt ccttgttgta aaacgacggc
     1801 cagtgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     1861 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     1921 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     1981 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     2041 aggacgaaac accggcctcc caaagtgcgt tttagagcta gaaatagcaa gttaaaataa
     2101 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcatggtcat
     2161 agctgtttcc taacgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     2221 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccaattccga
     2281 tcatattcaa taacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
     2341 ctgaagagga gtttacgtcc agccaagctt aggatctcga cctcgaaatt ctaccgggta
     2401 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     2461 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     2521 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     2581 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     2641 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     2701 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     2761 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     2821 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     2881 tctccgggcc tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc
     2941 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     3001 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3061 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3121 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3181 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3241 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3301 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3361 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3421 tccgcgcccc acaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3481 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     3541 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataagctga
     3601 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
     3661 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
     3721 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
     3781 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
     3841 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
     3901 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
     3961 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
     4021 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
     4081 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
     4141 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
     4201 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
     4261 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg
     4321 aggggcagga ggggcaggag gggsaggagg ggsaggagsa ggaggagggg saggagsagg
     4381 aggaggggsa ggaggrgsag gaggggsagg agsaggagga ggggsaggag saggaggagg
     4441 ggsaggaggg gsaggagsag gaggaggggs aggaggggsa ggaggggsag gagsaggagg
     4501 aggggcagga gsaggaggag gggcaggagg ggcaggagsa ggaggagggg caggaggggc
     4561 aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg caggaggggs
     4621 aggagcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg
     4681 aggggcagga gcaggagggg caggagcagg aggggsagga gcaggagggg saggaggggs
     4741 aggagsagga ggggsaggag gggsaggags aggaggggsa ggaggggsag gagsaggagg
     4801 aggggsagga ggggsaggag saggaggagg ggsaggaggg gsaggagsag gaggggsagg
     4861 aggggsagga gsaggagggg saggaggggs aggagsagga ggggsaggag gggsaggagc
     4921 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg
     4981 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg
     5041 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg
     5101 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc
     5161 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga
     5221 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc
     5281 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca
     5341 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg
     5401 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca
     5461 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag
     5521 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct
     5581 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc
     5641 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga
     5701 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat
     5761 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat
     5821 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg
     5881 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat
     5941 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg
     6001 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt
     6061 gccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
     6121 aagaggttca ctagcggatc tcaattgggc atacccatgt tgtcacgtca ctcagctccg
     6181 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
     6241 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga
     6301 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag
     6361 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg
     6421 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
     6481 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
     6541 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     6601 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg
     6661 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac
     6721 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac
     6781 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     6841 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     6901 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
     6961 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
     7021 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
     7081 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
     7141 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
     7201 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
     7261 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
     7321 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
     7381 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
     7441 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
     7501 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
     7561 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
     7621 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
     7681 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
     7741 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
     7801 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
     7861 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
     7921 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
     7981 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
     8041 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
     8101 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
     8161 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
     8221 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
     8281 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
     8341 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
     8401 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
     8461 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
     8521 ggcggggatc gatcctctag taagggattt tgccgatttc ggtctattgg ttaaaaaatg
     8581 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     8641 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     8701 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8761 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8821 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8881 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9001 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9061 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9121 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9181 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9241 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9301 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9361 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9421 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9481 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9541 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9601 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9661 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9721 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9781 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9841 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10201 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10741 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10801 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10861 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10921 gcaacgcaat
//
