LOCUS       sequence-395350-       10403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Ptb
FEATURES             Location/Qualifiers
     source          1..10403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3184..3387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3338..3358
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3384..3408
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3618..3636
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3618..3635
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3649..3668
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3727..3750
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5497..5614
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5663..6173
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6092..6110
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6195..6794
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6195..6214)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6691..6711
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6922..7155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7227..7348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7264..7283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7318..7337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7388..7523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7543..7562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7544..7562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7572..7589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7572..7588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7581..7603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7730..8185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7817..7836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8027..8048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8211..8315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8316..8384,8385..9176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8534..8553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9347..9935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9836..9855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10089..10106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10223..10244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10259..10264,10265..10282,10283..10289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10297..10313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10302..10324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10321..10337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10321..10337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10356..10376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10358..10376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt actgatgaat
     1621 cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt gtcatatgat
     1681 caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc gatattttac
     1741 acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac agcttaacgt
     1801 tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt ggccgtaacc
     1861 tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg ttaggtaatc
     1921 gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt atctgttcgg
     1981 gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa aaacggctta
     2041 tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat gagaaagcgt
     2101 tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc gaccttgcga
     2161 gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt aaagtgccat
     2221 ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga ggaaaagtac
     2281 aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat gatatgtcat
     2341 ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca atctcatgcc
     2401 aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg acacggactc
     2461 attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca tggattctag
     2521 caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc tattcgaagc
     2581 gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga ctaggcctac
     2641 gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc gccctgatgc
     2701 ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg gacaagcact
     2761 tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta ggcatggaag
     2821 ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct gcaaccctac
     2881 tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg gatctctcag
     2941 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     3001 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccatatgcca
     3061 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3121 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3181 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     3241 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     3301 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     3361 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3421 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     3481 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
     3541 gtctataggc ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta
     3601 tgtatcatac acatacgatt taggtgacac tatagaataa catccacttt gcctttctct
     3661 ccacaggtgt ccactcccag gtccaactgc acctcggttc tatcgattga attcaccatg
     3721 gaaatggact acaaggatga cgatgacaag ggtggtggtg gtggtgcgtc gacgatggac
     3781 ggcatcgtcc cagacatagc agtcggtaca aagcggggat ccgacgagct cttctccacg
     3841 tgtgtcagca acggcccctt catcatgagc agctctgcct cagcagccaa tggaaacgat
     3901 agcaagaagt tcaaaggtga caacaggagc gcaggagtcc cttccagagt catccatgtc
     3961 agaaagctgc ccagcgatgt cactgagggc gaggtcatct ccctagggct gccctttgga
     4021 aaggttacca accttctcat gctgaagggg aagaaccagg ccttcattga gatgaacaca
     4081 gaggaggctg ccaacactat ggttaactac tatacatcgg tggcgccagt gcttcgtgga
     4141 cagcccatct acatccagtt ctccaaccac aaagagctca agaccgacag ctcgcccaac
     4201 caggcacgtg cccaggcagc cctgcaggct gtaaactccg tccagtctgg aaacctggcc
     4261 ttggcagcgt ccgctgctgc cgtggatgca ggaatggcaa tggcagggca gagcccagtg
     4321 ctcaggatca ttgtggaaaa ccttttctac ccagtgaccc tggacgtgct gcaccagatc
     4381 ttctctaagt ttggcaccgt cctgaagatc atcacgttca ccaagaacaa ccagttccag
     4441 gcgctgctgc agtatgctga ccctgtgagc gcccagcatg ccaagctgtc cctggatggc
     4501 cagaacatct acaacgcctg ctgcacgctg cgcatcgact tctccaagct caccagtctc
     4561 aatgtcaagt acaacaatga taagagcaga gactacactc gacctgacct gccctctgga
     4621 gacagccagc cttcactaga ccagaccatg gcagcagcct ttggtgcgcc cggcataatg
     4681 tcagcctctc cgtatgcagg agccgggttc cctcccacct ttgccatccc tcaggccgca
     4741 ggcctctctg tccctaatgt ccatggagcc ttggcccccc tggccatccc gtctgctgct
     4801 gctgctgctg cggccagccg cattgccatc ccagggttgg caggtgctgg gaattctgtc
     4861 cttttggtca gcaatctgaa ccctgagaga gtcacacccc aaagcctctt tattctcttc
     4921 ggcgtctacg gtgatgtgca gcgggtgaag atcctgttca ataagaagga gaacgcactt
     4981 gtgcagatgg cagacggcag ccaggcccag ctggccatga gccacctgaa cgggcacaag
     5041 ctgcacggga agtcagtgcg cattacactg tccaagcatc agagtgtgca gctgcctcgg
     5101 gagggtcagg aggaccaggg cctgaccaag gactatggca gctccccgct gcaccgcttc
     5161 aagaaaccag gctccaagaa cttccagaac atctttccac cctcagctac cctgcacctc
     5221 tccaacatcc cgccctctgt gtcagaggac gacctcaaga gcctcttctc cagcaacggt
     5281 ggtgtggtca aaggcttcaa gttcttccag aaggaccgca agatggcact gatccagatg
     5341 ggctctgtgg aggaggctgt gcaggcgctg attgaactgc acaaccatga cctgggcgag
     5401 aaccaccacc tgcgagtgtc cttttccaag tccaccatct aggcggccgc agtgagaatt
     5461 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     5521 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5581 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     5641 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     5701 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     5761 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     5821 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     5881 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     5941 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     6001 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     6061 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     6121 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     6181 caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     6241 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     6301 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     6361 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     6421 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     6481 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     6541 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     6601 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     6661 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6721 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     6781 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     6841 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6901 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6961 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7141 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7201 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7321 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7381 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7441 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7501 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7561 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7621 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8161 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10321 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10381 aaaagctgga gctgcaagct taa
//
