LOCUS       sequence-395365-        7251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains WRKY17 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP40
FEATURES             Location/Qualifiers
     source          1..7251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6837..6856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6838..6856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6866..6888)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7082..7101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7201..7223
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgtctcgcag aggttattta tctacttggt
     2281 ttaaattgaa aactataaac caaattacgt cttagataac ttctataatt cttgccaatc
     2341 aacaagcaat agtaattagt aaaatttaat attttagttt aaattatgat cctcggatat
     2401 aacaaacaac tcacaagtag agtgggttga ctcagaacgc aatatgacac aataatacta
     2461 gatgaaatat tttattttgc actgacaata atattaatat tttttttttt ggtaaaggca
     2521 cgataagatt agttaggtgt ggattattgg gaaatatgat aatgtgtgga gataagtgag
     2581 aaaagattta gaaatggtgg atgttttatg tattggtcga accaaaagaa tcaaatcctc
     2641 aaccaaatca tcttacgtga tacgaccatg cctcaggtat tattttaagt tcattttgcc
     2701 cgtaaagtca agcattaaga gaagaatacc ctattacttt cacgtgctaa gatatattga
     2761 ttcagttaca tactggttca aatggtgata tagaaaatgt tattaattat cgtagaataa
     2821 cttcctaaaa gactcgtgac agattcagca tatcaaacgg agagtaagtt ccaagggttt
     2881 gttttgaagg ggatgatctg caccgcttcg taatggttcg aattcatatt ttggtgcatt
     2941 taactgagat tgagagaagc aagagtttgg aaggacaaat cgaaaaagat ttcaattttt
     3001 ttttgaaaac attgttttga tgactaaaat gacttatttt ctacctgaat tcatggttta
     3061 gggtaaaatt ttcaaagata taaaatcaca tacttagaat ttcaaaccaa atttctacta
     3121 atttacgttt aaccggtcaa attctgtaaa gaatcctaat cttcattctt ttaagatcat
     3181 aatcagctat ctaaagttta tgaagaccat tcattgtttt accaagtcct tgttcctagc
     3241 ttaaatcttc attttcattt ttgaaccatt ttgatttccc ctatatctag aactcaagac
     3301 atacaactta tatcatacaa tctaaaaata caattaaatt ctctattacg attttatact
     3361 aaaacatcaa ccaaagtaac cataattttt tatgaataaa aatattctat gcaacaaaaa
     3421 aattgtttta cagcaaatgc tttatagtaa tcaagaggtg gaattcgaaa tctgagatag
     3481 cactttgtaa cctaagataa aaattagtgt tacgggtttg aagtcaatag taaaaacatc
     3541 tttcgtaaga tcatattctt ggcgttcgta tagtggtgag actgtttcgt acacttttgt
     3601 taataactcc tatgaatctc caaggcagaa tggaggaatt tcacaaaaag aaaaatctca
     3661 atatcatttc tgatgaaatt gatggtcaga caaataaaaa caaatttccg accggtaaaa
     3721 aatttaattc tctaactttt aaattttttt aaacgtaatc gaaaattctt taaaaaaata
     3781 ttaagttaat ttttttattt taaaaatata atctgagttt attaaaaaat taatcattat
     3841 ctcatagata ttaatacatt taccaataat ttatctcgtg gaggttacat tgacttctac
     3901 aaactattca aatcgataac tctaattcgc taagtaatcc ccaaatcttt tctcagtatc
     3961 tttggcataa ataaagccaa atcttctcga gtgaatagca ataattatac atcatggctt
     4021 atatattgta tttttctgat aacaaatgtg cattatttct atcatccact aactaataat
     4081 taaaaattca atattgaccg tactattata aaaaaaaaaa acaatggttg gaagtttgaa
     4141 atacgtaaac aacaaaaaaa caaaaaaaaa taaaacttcg ttcgttagta ccgtatgaat
     4201 tcatagatac aacatataca aaataagtat tttacccaac atgtgtcttc tttcactggt
     4261 ttgatctcat tcgttttgca tgggctaaca aaacaattat ttcaaatacg cgaagattat
     4321 tatttctatg cgaccatttt atagaaaaga taatttagat aagatataaa ggagacattt
     4381 ttagaggaga taacattgaa atatatagta taatatattt ttagagattc ataaattata
     4441 aatttagaaa aggacattaa aatttgatat agaaaaggaa tttaaaagta agtaaaagtt
     4501 tacaaaatca gaaatgaaaa aataaaatgc ttttaaaaaa aatgtagacg tgcacgacaa
     4561 tcatatatat tagtcggtta ggtcaacgat tcccatgtcg ctaatttata atgcattccg
     4621 ttgacaaata tttaaaaatg tttcaattat acatcttgag gtctgttttt gcacacataa
     4681 cacgtcatat atcattgaca aagaaaacac gtcgtaattt tctttttctt tttctttcat
     4741 gtagtcttct tggcttaaaa gaattcagct aagtaagtat ggttgatatt ttcttctaca
     4801 agtgataaaa ctttggaatt tttgaataag tataattaca agaaaacact gcatataaca
     4861 actacaagaa aacactgcat ataccaaaat tatcgaaaca tgtatattcc gacaaactga
     4921 aatcaccgga aaacggtggt gtattgaaac aaagagttgt tttaactgaa gtttgaaagt
     4981 aaacaaaaga ttaaaaagta atttatagta atgttaatat ttactcatgt ttctgctttg
     5041 gtgattagta ataaatcttc taaaatctat atgccatttt ataaatgcga ttaaaaattt
     5101 accaattaaa ccttatttag aaaaatgtag agaaaacttc aaattatctc ctcaagtagg
     5161 attagatcga gctgcaaatt ggctcttttt attcatttaa tttcctccat tcctaactgt
     5221 attggtattg tttactttat ttctataagc aacaaaaaaa aaagaatctt gtggacaggt
     5281 gacaatttat gagtcagcca gaattgccac gtgtcggaag atcagttgcc gtggtaaaag
     5341 gtgtttttta gattctcaaa aaaaaagtag ttaaagagaa taatatcaaa gagtcaactc
     5401 cacagttcac agatccaccg ttggatctaa cttttggatt atcaacggcc cagataatag
     5461 ttaaccggaa tcactcggtt accgaggtag tcaatagttc tgtgttttaa aaagaaatgc
     5521 gtccatgtga tcacttttat tttatttgta gtcagctttt gttttcctac tttgtcgttt
     5581 ttctttatcg ctgttctcaa ctaaagactt gtagactata ctaataagat atagtcaacg
     5641 attgtcgctg ttattatagc ctttaatctc tattaatgat tgttaattgt tccacaatct
     5701 gtgtagatta attaggtaaa attaaaagtc aagaaaagat tgttttcaat ttactagttt
     5761 tccagtcaag acgttttcta gaaggctatg aggtaagaaa aaaaaaatat atattaggtg
     5821 caaagttaat atgggagtca aatattattt tcaaataatt attattgtca atgcactgca
     5881 aaattagtaa taattctaga aagaaattga aataataaaa atatgctgca aacacgagtt
     5941 tggtatgtgt gcaccatata atattgacaa gttgtagtgt tggttgaagc taaaaataac
     6001 acaaaaacat aaaaagaaca tgaataacgc ctaaaactta acacgaataa aataaaaaaa
     6061 cataaaatat attatcaaaa gtcaaaatca caaaagaaat taataccatt ttacaattag
     6121 ctgaaaattt attctatcaa aatccagtcc tattaaagaa aaatatgtga tttgagatga
     6181 aattaatatt acatattttg atttggttca agaataaatt atggattttg gttaaagacc
     6241 ttctaatatt ccaaagaaac aaaaaaaaat cagcccaatt gttcatacaa ataaaaacaa
     6301 ttcattacct ttaattttaa atttattgac ttgaacttga agacataaga tacctaataa
     6361 aagaaaaaat agatatgaga ctttaaaaaa gctttatgat tttctttaga caccatcctt
     6421 taatgttttt tatttgactt tttgtttctt tgaaattcct ttaccaccat tttccccaaa
     6481 ttcaagttta cgcacaatga ttcctttatt ttaaagacac gattataaat tcttgctttg
     6541 cacaaaagaa gaccctacat atctcacaac tcaagcagac caaactttga ttactttatt
     6601 ccatagaaat cttcaactca atctcagccg ttagatctaa agcaccgatt tgactaaact
     6661 ccatcttaaa cctactcaac cggtcactcg gtcacaccca taaccccata tatcacgcca
     6721 acgccattct tttttcttcc agtttcgctc tctcattcat caaaaaaaac ttgcacatct
     6781 tctcagatct tcaagtttct cctctggttt ctcatcaaca tgagaccgaa ttctcgccct
     6841 atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6901 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6961 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     7021 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     7081 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7141 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7201 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
