LOCUS       sequence-395366-        5199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains MIK2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP15
FEATURES             Location/Qualifiers
     source          1..5199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4785..4804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4786..4804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4814..4836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5030..5049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5149..5171
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgtaaataac gttgaactcg cgggttttcc
     2281 ggattgcctc tatacatatg ataaagttgt aacaagaaac gggtcatctc gtctgaccaa
     2341 atgattttct aatcgagaaa acaaattaaa acatgtattc aatcaaaaaa ctcaaaaaaa
     2401 taaaaaataa aaaaaatgac gatgccatga aatactcaga gaattacact agctaatgaa
     2461 tggatgaaat gatagatcat acttacgttg attcttgatt ttgattttga ttttcgtcag
     2521 tttgggtgga tgtcgtaatt gagagaatag agttttaata tattgattag ggttttctga
     2581 taagaataag atatataggg tttgggtgga agacataatt cttttaatta attaaaaaaa
     2641 aaaaaaatct aaccgctacc gcccacaaac gtttgtggga aggagatttt gaaatctggc
     2701 gatttcgaac ggtccaaagc ggtatctagc agtttgtatg attggtgcca atcgctaaca
     2761 accgctacca cccgcaaatg cagcgtttgc ggatggcaga gggaaaacca atcaagccct
     2821 taattgttcc ttaaaaacaa gagaaaacgc gttacaaaag ctcaaaggac tgttttcctc
     2881 ttcttccttt tttttatata taataatctt attttaacag aattatattg aataataaaa
     2941 acgaaaacta aagaagagat gacatacgtg atatgtcata gaataagaag aatatgatat
     3001 taagaagagt taattaaaaa ataaaccata agatgttctt aaaattataa ctttttatga
     3061 tgtaaaaata agtatcagat aatacacaat tcggattttc aacgaattgg gttattgtta
     3121 actttttatg agaatgattt cgttatggat tataaatttt tggttttata tgcgttttaa
     3181 atgattgaat agttaggatt ttgatgatgg tcgagatgat tacaatttac gtttgaaaaa
     3241 ttaagtgttc gtgtaaacaa tggtggagct tttctttctc ggccaataga gtcacgtaaa
     3301 tcttattacc tactagtcat gcaagctaac ctttgttatt cagtcttcgc actaagtcta
     3361 tacctcaatc aatcaattct tctatttttt gtcaagtttc tagttgtctt tgaatttctt
     3421 ttctaatttt gtctggcttc ttctatacga aaatgacttg gtttcacaca ttattttcca
     3481 aagaaaatta taacgaatgc tatcttaata actatttagg atttacatac ttgaactaag
     3541 aaataatata ttaacaattc aatacaaaga aaaaaaaaac atacaataca ataagaaggt
     3601 ccatgagatt acattgaggg gaccagttga ttgtacatga ccataccttg tgcaagactt
     3661 caaaagtcaa tcctttttgt cgtagatctc ttccctaaaa gttgaatttc tattattttt
     3721 accaaagtat atttgacaaa aataaaacta aacctctttt gttatttaca gtgataagga
     3781 aattcgagat taaaaaataa aaaaaaagaa taataataat aattcgagat gttaaatcta
     3841 catatggtga tcttgactct tgaggcgtcc gtgttttatt taagtttcgt attttttagg
     3901 tttgaactta cgttaaaggg acttccgcta agcattctag tcagtctgaa gatctttttt
     3961 tcccttactg ctcttgtttc tagccatgtt ctgcttaggt gagtcttttt tatcgagttg
     4021 attcttaagt aacctttact acttaagaat ttagtgatga tttgattttc gaatgtatat
     4081 actttcataa ttctttagct tttttggtat gggacggtat gggaccatat ttttagaatt
     4141 tactttgtaa tttctatatc tttcattttt tgaaatgata tttacattct tataagaaaa
     4201 atagtttctt attttatggt acaagttgtt aaaaaatcac acaataaagt taagagatat
     4261 ttttaagaaa gatttgtatt atattactat actaaaagta acatcaatgc attttcccat
     4321 tgttgaaaaa actaattaat cttatcgtcc aaatgaacat gactaaccca tattttattt
     4381 gctaatgaaa aattgaccat cgttgtcagt ccatataaat ccaccaatcc tgccgtcaat
     4441 aacttttata taagtgtttt tttttatata cattataata taacattcaa agaactcata
     4501 cattatcttt atacaaaagt agaaaacaac aacttaaaca agtcagtaaa ctatttttat
     4561 attaatgtga atgtgtgtgt gaagtgataa tttccgaaga ttttgtttga caaagtaaac
     4621 ggctaataat taaagagaga attcaagata ttgacttatt tgtcctctat taacaagtga
     4681 caacccatag tcaactatca cggactagtc aacgaaatca attttcagtt tataaaagac
     4741 cgtagagaga taatctgcaa ctgtaacatg agaccgaatt ctcgccctat agtgagtcgt
     4801 attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4861 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4921 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     4981 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5041 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5101 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
