LOCUS       sequence-395370-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human DNA repair protein REV1 with an N-terminal HA tag in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ashton NW, Jaiswal N, Cestari Moreno N, Semenova IV, D'Orlando DA, 
            Teatin Latancia M, McIntyre J, Woodgate R, Bezsonova I
  TITLE     A novel interaction between RAD23A/B and Y-family DNA polymerases.
  JOURNAL   J Mol Biol. 2023 Nov 5:168353. doi: 10.1016/j.jmb.2023.168353.
  PUBMED    37935254
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2023 Nov 5:168353. doi: 10.1016/j.jmb.2023.168353."
COMMENT     Sequence Label: pCMV6-AN-HA_WT REV1
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1023..1049
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4879..4895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4906..5528
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5105..5124)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5552..5572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5557..5886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5737..5872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5953..6747
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6007..6026)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6617..6636
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6921..7042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6958..6977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7012..7031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7091..7107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7104..7126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7115..7131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7139..7145,7146..7163,7164..7169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7184..7205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7322..7339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7493..8081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7573..7592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8252..9043,9044..9112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8875..8894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9113..9217)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9244..9672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9331..9350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9541..9562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccacca
     1021 tgtaccctta tgatgtccca gattatgctg cgatcgccca tatgcggaga ggcggatggc
     1081 ggaaacgggc tgaaaatgat ggatgggaga catggggcgg ctatatggcc gccaaggtgc
     1141 agaagctgga ggagcagttc agaagcgacg ccgccatgca gaaggatggc accagctcca
     1201 caatctttag cggcgtggcc atctatgtga acggctacac agacccctcc gccgaggagc
     1261 tgaggaagct gatgatgctg cacggcggcc agtaccacgt gtactattcc cggtctaaga
     1321 ccacacacat catcgccacc aacctgccta atgccaagat caaggagctg aagggcgaga
     1381 aagtgatcag accagagtgg atcgtggaga gcatcaaggc cggcaggctg ctgtcctaca
     1441 tcccttatca gctgtacacc aagcagtcta gcgtgcagaa gggcctgtcc ttcaatccag
     1501 tgtgcaggcc agaggaccca ctgccaggac cttctaatat cgccaagcag ctgaacaatc
     1561 gggtgaacca catcgtgaag aagatcgaga cagagaatga ggtgaaggtg aacggcatga
     1621 acagctggaa cgaggaggac gagaacaatg atttctcttt tgtggatctg gagcagacca
     1681 gccctggccg caagcagaac ggcatcccac acccccgggg cagcaccgcc atctttaatg
     1741 gccacacacc atcctctaac ggcgccctga agacccagga ctgcctggtg ccaatggtga
     1801 atagcgtggc ctcccgcctg tctccagcct tctcccagga ggaggataag gccgagaaga
     1861 gctccaccga ctttcgcgat tgtacactgc agcagctgca gcagagcacc cggaacacag
     1921 acgccctgag gaatccccac cgcacaaaca gcttctccct gtctcctctg cacagcaata
     1981 ccaagatcaa cggagcacac cactccacag tgcagggacc ctctagcacc aagtctacat
     2041 cctctgtgag caccttttcc aaggccgccc cttctgtgcc aagcaagccc tccgattgca
     2101 atttcatctc taacttttat agccactccc ggctgcacca catcagcatg tggaagtgtg
     2161 agctgaccga gttcgtgaat acactgcaga gacagtctaa cggcatcttt ccaggcaggg
     2221 agaagctgaa gaagatgaag accggcagaa gcgccctggt ggtgaccgac acaggcgata
     2281 tgagcgtgct gaactccccc aggcaccagt cttgcatcat gcacgtggac atggattgtt
     2341 tctttgtgag cgtgggcatc aggaacaggc cagacctgaa gggcaagcca gtggcagtga
     2401 cctccaatag gggaacagga agagcacctc tgaggccagg agcaaaccca cagctggagt
     2461 ggcagtacta tcagaataag atcctgaagg gcaaggccgc cgacatccca gatagctccc
     2521 tgtgggagaa tcccgacagc gcccaggcca acggcatcga ttctgtgctg agccgcgccg
     2581 agatcgcctc ttgcagctac gaggcccggc agctgggcat caagaacggc atgttctttg
     2641 gccacgccaa gcagctgtgc cctaatctgc aggccgtgcc ctacgacttc cacgcctaca
     2701 aggaggtggc ccagaccctg tatgagacac tggcctccta cacccacaac atcgaggccg
     2761 tgtcttgcga cgaggccctg gtggatatca cagagatcct ggccgagaca aagctgacac
     2821 ctgacgagtt tgccaacgcc gtgagaatgg agatcaagga tcagacaaag tgtgccgcct
     2881 ctgtgggcat cggcagcaat atcctgctgg caaggatggc aacccggaag gcaaagcctg
     2941 acggacagta tcacctgaag ccagaggagg tggacgattt catcagggga cagctggtga
     3001 ccaacctgcc aggagtgggc cactccatgg agtctaagct ggccagcctg ggcatcaaga
     3061 cctgcggcga tctgcagtat atgacaatgg ccaagctgca gaaggagttc ggccccaaga
     3121 ccggccagat gctgtacagg ttttgtcgcg gcctggacga tcggcctgtg agaacagaga
     3181 aggagagaaa gtccgtgtct gccgagatca attacggcat caggttcacc cagccaaagg
     3241 aggccgaggc ctttctgctg agcctgtccg aggagatcca gcggagactg gaggccaccg
     3301 gcatgaaggg caagagactg acactgaaga tcatggtgag gaagccagga gcacctgtgg
     3361 agacagccaa gttcggcggc cacggcatct gcgacaacat cgcaaggacc gtgacactgg
     3421 accaggcaac cgataatgcc aagatcatcg gcaaggccat gctgaatatg tttcacacaa
     3481 tgaagctgaa catctccgat atgcggggcg tgggcatcca cgtgaaccag ctggtgccta
     3541 ccaacctgaa tccatctaca tgcccaagca gaccctccgt gcagtctagc cacttccctt
     3601 ctggcagcta ctccgtgagg gacgtgtttc aggtgcagaa ggccaagaag tccacagagg
     3661 aggagcacaa ggaggtgttc cgcgcagcag tggacctgga gatcagcagc gccagccgga
     3721 cctgtacatt cctgccccct tttcccgccc acctgcctac ctcccctgac acaaataagg
     3781 ccgagagcag cggcaagtgg aacggcctgc acacccccgt gagcgtgcag agcagactga
     3841 atctgtccat cgaggtgcct tctccaagcc agctggacca gtctgtgctg gaggccctgc
     3901 cacccgatct gagggagcag gtggagcagg tgtgcgcagt gcagcaggca gagagccacg
     3961 gcgataagaa gaaggagccc gtgaatggct gtaacaccgg catcctgcca cagcctgtgg
     4021 gaacagtgct gctgcagatc ccagagcccc aggagtccaa ttctgacgcc ggcatcaacc
     4081 tgatcgccct gccagccttc tcccaggtgg accccgaggt gtttgccgcc ctgcctgcag
     4141 agctgcagag agagctgaag gcagcctacg accagaggca gagacagggc gagaacagca
     4201 cacaccagca gagcgcctcc gcctctgtgc caaagaatcc tctgctgcac ctgaaggccg
     4261 ccgtgaagga gaagaagcgg aacaagaaga agaagaccat cggctccccc aagagaatcc
     4321 agtctcctct gaacaataag ctgctgaata gcccagcaaa gaccctgcct ggagcatgtg
     4381 gctccccaca gaagctgatc gatggcttcc tgaagcacga gggacctcca gcagagaagc
     4441 cactggagga gctgagcgcc tccacctctg gagtgccagg cctgtctagc ctgcagtccg
     4501 accctgcagg atgcgtgcgc ccccctgcac caaatctggc cggcgccgtg gagtttaacg
     4561 atgtgaagac actgctgcgg gagtggatca ccacaatcag cgaccccatg gaggaggata
     4621 tcctgcaggt ggtgaagtat tgtaccgacc tgatcgagga gaaggacctg gagaagctgg
     4681 acctggtcat caagtacatg aagaggctga tgcagcagtc cgtggagagc gtgtggaaca
     4741 tggccttcga ctttattctg gataatgtcc aggtcgtcct gcagcagact tacggctcaa
     4801 cactgaaggt cacctaagga tccaagcttc tcgagacgcg ttaagcggcc gcactcgagg
     4861 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     4921 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     4981 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     5041 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     5101 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     5161 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     5221 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     5281 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     5341 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     5401 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     5461 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     5521 gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
     5581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5641 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5701 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5761 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5821 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5881 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     5941 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6001 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     6061 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6121 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6181 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6241 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     6301 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6361 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6421 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6481 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6541 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggccgac
     6601 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6661 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6721 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6781 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6841 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6901 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7021 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     7081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7261 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9241 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9661 ttaacgcgaa tttt
//
