LOCUS       sequence-395372-        4736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains MYB15 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP71
FEATURES             Location/Qualifiers
     source          1..4736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4322..4341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4323..4341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4351..4373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4567..4586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4686..4708
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgatgaattt gaataaacta aacaaaatta
     2281 aataattgat gttttggttt cataccatat caaatctgag aaagaaacaa aaagatagag
     2341 atgaaagtga ttttcacatt aagctgtgga ttatgtttac aacgaaatac atataaatat
     2401 tatatttatt aggctagcaa aaccttaact aaacccaaac atgatgcaat tgataatttg
     2461 tctttctgac ccaataaact gggtacataa attttatatg tcagtatgct tcatgttaca
     2521 gtactttaat tatttaacac tagtaacgca aaaaagattt tttaataaat gatcatttaa
     2581 tcattatcat tcttcattaa acattattta ttgttttcta ataaaatata atgatggttt
     2641 aattgttagt gtaaatttat atggtaaaac taaaacctgt aggtaagcct cttttcagtc
     2701 ttttgttgac cagaaacttt gaacaataga gttctatttt aacaaatact aacaaaatac
     2761 agaagaaggg taaagtttta ggttgaaact taaaagggtg cgatacgtgt gaacgcgttg
     2821 gtcaacaaat actatcacga gctgttctct ttcccgcgga cagatattga ttttttcgtg
     2881 ttctattttt ggattttgtg gctggtgtaa taataatatt aaagcatata tactagtata
     2941 aaactggtaa tttttcgtag aggattgttt tctcaaagcc caaacagtag ttgcaaattt
     3001 gtaataacca gattttgaag taaaataaaa tggtgaggaa attttagaat aatttattta
     3061 tcaaaaaaaa actttagaat atgttacata tttggtagtt atagtattgt atcaaataca
     3121 catattaatc gaattgtttg ataaaatgga agtaaattat ttttgactga actatgtagc
     3181 acatatttat tttatatgaa tctcaaaatc atttatataa tttatgaaaa agttagtttt
     3241 ttttctattc gatacgttaa caattaatta tgaccaataa attgttttaa ggcattcagc
     3301 agtctaaaaa taaagactga aaatggcgtc ataataaacg taaatgcagt aaatattatt
     3361 ccctctattt ttgaatattt gtagtttaaa atttttgcac acaaattaag aaaacataca
     3421 aatctcattt aatttatctc tttcttataa aaatattatt aattacaatt aatccaacca
     3481 ataaaaaaat atgatataaa atataattgg ttaaaaacta ttaaacacat ttaattttgc
     3541 atagaaaagt gaaaacaaca cttatagtaa aaaaaataaa aaatctttaa attacactta
     3601 attagaaata gaaagaatac aaaacgtacg gatgttgtta acctaaacac gattactacc
     3661 gagactaaag atgtctaagt ttaatactaa gaaaagtaaa acaatttttc gagcagatat
     3721 atgaatagtg attttttggg tcaacacata atccacccaa cgttgatttt tgtttgtcgt
     3781 ttacaatctc taaaacgttt attttctaag aaatatacgt gtgggctcat tttaatgccc
     3841 ttcgtgcgtc tacccgacga ctttgtttac tcgaaactat atcacacact acaccacata
     3901 tttcaacatc acactgtata cacacattta tatatacatg cagaccgatt attataataa
     3961 tattttctcc ggtccctaaa acatatatat cattcattcc cacatgagat ataatattat
     4021 gtaaaacata cttttgacta tgttgccaat aatcaaaaag acatctctct ctctctctct
     4081 cacatcctat tttccaagaa gctcaccaat gcttttgaat tctcccacat gtcttttgac
     4141 aattttagta taaatactca aaccccttag tcagtcattc tcatgttcat caacagcatt
     4201 tgatctcaag ctcataggaa tttgatctcc acattatatc ttgatcatca ccacaagaaa
     4261 aacatttcaa cttcttttat cagcaatcac aaatcaaaga gaacatgaga ccgaattctc
     4321 gccctatagt gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4381 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4441 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     4501 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     4561 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4621 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4681 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
