LOCUS       sequence-395381-        8867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the Human TrkC receptor under the Moloney mouse sarcoma 
            virus LTR. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Urfer R, Tsoulfas P, O'Connell L, Hongo JA, Zhao W, Presta LG
  TITLE     High resolution mapping of the binding site of TrkA for nerve growth
            factor and TrkC for neurotrophin-3 on the second immunoglobulin-like
            domain of the Trk receptors.
  JOURNAL   J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 
            10.1074/jbc.273.10.5829.
  PUBMED    9488719
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.273.10"; journalName: "J Biol Chem"; date: "1998-03-6- 
            6"; volume: "273"; issue: "10"; pages: "5829-40"
COMMENT     Sequence Label: pMEXneo-TrkC
FEATURES             Location/Qualifiers
     source          1..8867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..248
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(510..526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(510..526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(523..545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    534..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(558..564,565..582,583..588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    603..624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(741..758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(912..1499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(991..1010)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1670..2461,2462..2530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2293..2312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2531..2635)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2703..2721
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     3094..3116
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3108..3125
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3109..3125
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6436..6570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6460..6479)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6514..6533
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6995..7015)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7145..7210
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(7630..8424)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7741..7760)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8351..8370
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8781..8867,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(8795..8867,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      301 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      361 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      421 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
      481 catacggatc ggaattaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
      541 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      601 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      661 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      961 agtagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2641 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2701 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     2761 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     2821 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     2881 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     2941 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3001 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3061 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3121 ccagtgccaa gctaattctc tgtactctat atttaagttt cctataaatc tatagctttt
     3181 ctaaaaataa aatctattta aaaattactt gaatgaaagg taaacacgcc tctttcacac
     3241 agtctgatgc aaagtctcat caagaattgg gcagtcctgg gaagttggaa ccctgtaaat
     3301 acggactcca ccgtgcagac aatgccatgt agagaagcgt gttcccaaga tcccagcttc
     3361 cccactctgg gtccttcccc tcatagatat aaacgtgaaa gaccccagcc gtaggtttgg
     3421 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     3481 gaagttcaga tcaaggtcag gaacagagag acagctgaat atgggccaaa caggatatct
     3541 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaattgggg
     3601 caaacaggat atctgcggta agcagttcct gccccggctc agggccaaga acagatggtc
     3661 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     3721 ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     3781 ttctgttcgc gcgcttccgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     3841 cgcgccagtc ttccgataga ctgcgtcgcc cagcttgcat gcctgcaggt cgactctaga
     3901 ggatccgcgt cggagatgga tgtctctctt tgcccagcca agtgtagttt ctggcggatt
     3961 ttcttgctgg gaagcgtctg gctggactat gtgggctccg tgctggcttg ccctgcaaat
     4021 tgtgtctgca gcaagactga gatcaattgc cggcggccgg acgatgggaa cctcttcccc
     4081 ctcctggaag ggcaggattc agggaacagc aatgggaacg ccaatatcaa catcacggac
     4141 atctcaagga atatcacttc catacacata gagaactggc gcagtcttca cacgctcaac
     4201 gccgtggaca tggagctcta caccggactt caaaagctga ccatcaagaa ctcaggactt
     4261 cggagcattc agcccagagc ctttgccaag aacccccatt tgcgttatat aaacctgtca
     4321 agtaaccggc tcaccacact ctcgtggcag ctcttccaga cgctgagtct tcgggaattg
     4381 cagttggagc agaacttttt caactgcagc tgtgacatcc gctggatgca gctctggcag
     4441 gagcaggggg aggccaagct caacagccag aacctctact gcatcaatgc tgatggctcc
     4501 cagcttcctc tcttccgcat gaacatcagt cagtgtgacc ttcctgagat cagcgtgagc
     4561 cacgtcaacc tgaccgtacg agagggtgac aatgctgtta tcacttgcaa tggctctgga
     4621 tcaccccttc ctgatgtgga ctggatagtc actgggctgc agtccatcaa cactcaccag
     4681 accaatctga actggaccaa tgttcatgcc atcaacttga cgctggtgaa tgtgacgagt
     4741 gaggacaatg gcttcaccct gacgtgcatt gcagagaacg tggtgggcat gagcaatgcc
     4801 agtgttgccc tcactgtcta ctatccccca cgtgtggtga gcctggagga gcctgagctg
     4861 cgcctggagc actgcatcga gtttgtggtg cgtggcaacc ccccaccaac gctgcactgg
     4921 ctgcacaatg ggcagcctct gcgggagtcc aagatcatcc atgtggaata ctaccaagag
     4981 ggagagattt ccgagggctg cctgctcttc aacaagccca cccactacaa caatggcaac
     5041 tataccctca ttgccaaaaa cccactgggc acagccaacc agaccatcaa tggccacttc
     5101 ctcaaggagc cctttccagt tgacgaagtg agtcccacac ctcctatcac ggtgacccac
     5161 aaaccagaag aagacacttt tggggtatcc atagcagttg gacttgctgc ttttgcctgt
     5221 gtcctgttgg tggttctctt cgtcatgatc aacaaatatg gacgacggtc caaatttgga
     5281 atgaagggtc ccgtggctgt catcagtggt gaggaggact cagccagccc actgcaccac
     5341 atcaaccacg gcatcaccac gccctcgtca ctggatgccg ggcccgacac tgtggtcatt
     5401 ggcatgactc gcatccctgt cattgagaac ccccagtact tccgtcaggg acacaactgc
     5461 cacaagccgg acacgtatgt gcagcacatt aagaggagag acatcgtgct gaagcgagaa
     5521 ctgggtgagg gagcctttgg aaaggtcttc ctggccgagt gctacaacct cagcccgacc
     5581 aaggacaaga tgcttgtggc tgtgaaggcc ctgaaagatc ccaccctggc tgcccggaag
     5641 gatttccaga gggaggccga gctgctcacc aacctgcagc atgagcacat tgtcaagttc
     5701 tatggagtgt gcggcgatgg ggaccccctc atcatggtct ttgaatacat gaagcatgga
     5761 gacctgaata agttcctcag ggcccatggg ccagatgcaa tgatccttgt ggatggacag
     5821 ccacgccagg ccaagggtga gctggggctc tcccaaatgc tccacattgc cagtcagatc
     5881 gcctcgggta tggtgtacct ggcctcccag cactttgtgc accgagacct ggccaccagg
     5941 aactgcctgg ttggagcgaa tctgctagtg aagattgggg acttcggcat gtccagagat
     6001 gtctacagca cggattatta caggctcttt aatccatctg gaaatgattt ttgtatatgg
     6061 tgtgaggtgg gaggacacac catgctcccc attcgctgga tgcctcctga aagcatcatg
     6121 taccggaagt tcactacaga gagtgatgta tggagcttcg gggtgatcct ctgggagatc
     6181 ttcacctatg gaaagcagcc atggttccaa ctctcaaaca cggaggtcat tgagtgcatt
     6241 acccaaggtc gtgttttgga gcggccccga gtctgcccca aagaggtgta cgatgtcatg
     6301 ctggggtgct ggcagaggga accacagcag cggttgaaca tcaaggagat ctacaaaatc
     6361 ctccatgctt tggggaaggc caccccaatc tacctggaca ttcttggcta gtggtggctg
     6421 gtggtcatga attccgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6481 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6541 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6601 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6661 atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt
     6721 tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc
     6781 tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta
     6841 taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
     6901 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
     6961 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
     7021 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
     7081 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
     7141 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
     7201 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
     7261 agaagtaagg ttccttcaca aagatccgga ccaaagcggc catcgtgcct ccccactcct
     7321 gcagttcggg ggcatggatg cgcggatagc cgctgctggt ttcctggatg ccgacggatt
     7381 tgcactgccg gtagaactcc gcgaggtcgt ccagcctcag gcagcagctg aaccaactcg
     7441 cgaggggatc gagcccgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     7501 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     7561 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     7621 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     7681 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     7741 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     7801 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     7861 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     7921 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     7981 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     8041 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     8101 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     8161 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     8221 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     8281 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     8341 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     8401 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct
     8461 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     8521 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     8581 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     8641 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     8701 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     8761 cttgcggcag cgtgaagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     8821 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaat
//
