LOCUS       sequence-395423-        9723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the beta-arrestin1 containing a C-terminal V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Zeghal M, Matte K, Venes A, Patel S, Laroche G, Sarvan S, Joshi M, 
            Rain JC, Couture JF, Giguere PM
  TITLE     Development of a V5-tag-directed nanobody and its implementation as 
            an intracellular biosensor of GPCR signalling.
  JOURNAL   J Biol Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107.
  PUBMED    37517699
REFERENCE   2  (bases 1 to 9723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2023 Jul 28:105107. doi: 10.1016/j.jbc.2023.105107."
COMMENT     Sequence Label: bArr1-V5
FEATURES             Location/Qualifiers
     source          1..9723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2478..2502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2510..2551
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2661..3249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2714..2734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3132..3143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3321..3554
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3632..3753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3669..3688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3723..3742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3793..3928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3948..3967)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3949..3967)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3977..3994)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3977..3993)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3986..4008)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4135..4590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4222..4241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4432..4453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4616..4720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4721..4789,4790..5581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4939..4958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5752..6340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6241..6260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6494..6511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6628..6649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6664..6669,6670..6687,6688..6694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6702..6718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6707..6729
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6726..6742
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6726..6742
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6761..6781
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6763..6781
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6809..7035
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7036..7216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7263..7388
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7881..8114
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8299..8343
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8574..8784
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8596..8792
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8643..8778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8824..9426
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8824..8843)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9320..9340
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9469..9586
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgagcga caaagggacc cgagtgttca agaaggccag
     1261 tccaaatgga aagctcaccg tctacctggg aaagcgggac tttgtggacc acatcgacct
     1321 cgtggaccct gtggatggtg tggtcctggt ggatcctgag tatctcaaag agcggagagt
     1381 ctatgtgacg ctgacctgcg ccttccgcta tggccgggag gacctggatg tcctgggcct
     1441 gacctttcgc aaggacctgt ttgtggccaa cgtacagtcg ttcccaccgg cccccgagga
     1501 caagaagccc ctgacgcggc tgcaggaacg cctcatcaag aagctgggcg agcacgctta
     1561 ccctttcacc tttgagatcc ctccaaacct tccatgttct gtgacactgc agccggggcc
     1621 cgaagacacg gggaaggctt gcggtgtgga ctatgaagtc aaagccttct gcgcggagaa
     1681 tttggaggag aagatccaca agcggaattc tgtgcgtctg gtcatccgga aggttcagta
     1741 tgccccagag aggcctggcc cccagcccac agccgagacc accaggcagt tcctcatgtc
     1801 ggacaagccc ttgcacctag aagcctctct ggataaggag atctattacc atggagaacc
     1861 catcagcgtc aacgtccacg tcaccaacaa caccaacaag acggtgaaga agatcaagat
     1921 ctcagtgcgc cagtatgcag acatctgcct tttcaacaca gctcagtaca agtgccctgt
     1981 tgccatggaa gaggctgatg acactgtggc acccagctcg acgttctgca aggtctacac
     2041 actgaccccc ttcctagcca ataaccgaga gaagcggggc ctcgccttgg acgggaagct
     2101 caagcacgaa gacacgaact tggcctctag caccctgttg agggaaggtg ccaaccgtga
     2161 gatcctgggg atcattgttt cctacaaagt gaaagtgaag ctggtggtgt ctcggggcgg
     2221 cctgttggga gatcttgcat ccagcgacgt ggccgtggaa ctgcccttca ccctaatgca
     2281 ccccaagccc aaagaggaac ccccgcatcg ggaagttcca gagaacgaga cgccagtaga
     2341 taccaatctc atagaacttg acacaaatga tgacgacatt gtatttgagg actttgctcg
     2401 ccagagactg aaaggcatga aggatgacaa ggaggaagag gaggatggta ccggctctcc
     2461 acagctcaac aacagatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2521 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaagt
     2581 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtgcgtgg tttctttata
     2641 tcataacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     2701 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2761 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2821 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2881 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2941 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3001 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3061 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3121 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3181 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3241 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3301 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     3361 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3421 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3481 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3541 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     3601 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     3661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3721 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     3781 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3841 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3901 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     3961 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4021 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4081 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4561 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     4621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5881 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6541 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     6601 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6661 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6721 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     6781 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     6841 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6901 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6961 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     7021 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7081 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7141 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7201 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7261 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7321 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7381 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7441 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7501 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7561 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7621 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7681 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     7741 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7801 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7861 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7921 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7981 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8041 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8101 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8161 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8221 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8281 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8341 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8401 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8461 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg
     8521 cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8581 gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8641 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8701 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8761 gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt
     8821 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     8881 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     8941 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     9001 gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg
     9061 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     9121 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     9181 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     9241 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     9301 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     9361 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     9421 agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     9481 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9541 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9601 ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag
     9661 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     9721 att
//
