LOCUS       sequence-395435-       17113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fly transformation vector expressing indicated Wnk variant under 
            tubulin promoter for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Jenny Lab
  TITLE     Jenny Lab Wnk Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 17113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAttB-Tub-HA-Wnk
FEATURES             Location/Qualifiers
     source          1..17113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    156..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    338..407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(626..644)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(665..681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(665..681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(678..700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    689..705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(713..719,720..737,738..743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    758..779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(896..913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1067..1655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1147..1166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1826..2617,2618..2686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2449..2468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2687..2791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2817..3272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2954..2975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3166..3185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3399..3421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3413..3430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3414..3430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3437..3456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3437..3455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            3480..7616
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3489..3507)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3959..4030,4432..4705,4780..5434,5496..5811,6032..6163,
                     6234..6848)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7623..7656
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             10324..10350
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagtcga cctcgaacgt taacgttaac gtaacgttaa ctcgagctag atcataatca
       61 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatgtcgagg tcgacctgca gcccaagctg
      301 ctagagatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
      361 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
      421 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
      481 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
      541 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
      601 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
      661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     2821 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2881 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2941 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3001 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3061 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3121 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3181 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3241 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     3301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3421 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc
     3481 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     3541 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     3601 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     3661 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     3721 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     3781 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     3841 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     3901 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     3961 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     4021 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     4081 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     4141 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     4201 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     4261 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     4321 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     4381 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     4441 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     4501 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     4561 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     4621 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     4681 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     4741 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     4801 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     4861 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     4921 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     4981 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaggccatg gtgcggatgc
     5041 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     5101 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     5161 gcggagaaag gaagcgtctg gcattcgcct ccgaggcact aaccgatccg ccgcttctga
     5221 tctgcgatga gcccacctcc ggactggact catttaccgc ccacagcgtc gtccaggtgc
     5281 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     5341 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     5401 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     5461 atctagcatt acattacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     5521 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     5581 tcccgtgatc ggatcgccaa gatatgcgac aattttgcta ttagcaaagt agcccgggat
     5641 atggagcagt tgttggccac caaaaatttg gagaagccac tggagcagcc ggagaatggg
     5701 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     5761 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     5821 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     5881 gttagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     5941 gttttgcaga aaataaataa atttcattta actcgcgaac atgttgaaga tatgaatatt
     6001 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt
     6061 tttgggccaa caactcacgc aagtgggcgt gatgaatatc aacggagcca tcttcctctt
     6121 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc ttgtttagaa
     6181 tacatttgca tattaataat ttactaactt tctaatgaat cgattcgatt taggtgttca
     6241 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat
     6301 actttctggg caaaacgatt gccgaattac cgctttttct cacagtgcca ctggtcttca
     6361 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc
     6421 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg
     6481 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc
     6541 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
     6601 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
     6661 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
     6721 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca
     6781 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca
     6841 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca
     6901 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
     6961 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
     7021 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
     7081 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
     7141 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
     7201 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
     7261 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
     7321 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
     7381 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
     7441 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
     7501 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
     7561 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactaggcta
     7621 gcataacttc gtataatgta tgctatacga agttatgcta gcggatccgg gaattgggaa
     7681 ttcgatatca agcttgcaca ggtcctgttc gataacgtcg tactcgggaa tcacctttaa
     7741 cttgtccgct attgggtatt tgtccttaaa cacattcgta atctctccca caatcattgc
     7801 cctggcctct gcaatgtaaa aagtatttat aaaatatcgc tggctctaaa gaaaacatat
     7861 taatcaaagt attgaggctt tatcaatcta acatttacaa gtagtcagac cacaattttt
     7921 agactgcact tctgcgtaag taaatgaaat gaaatggtga cctactcttg ggtagccgta
     7981 ctgttatatg ctgacgagcc ttcacctggg cctgcttttt ctcctgtttc gtctgatgcg
     8041 tcggatgcgg ctggactggc tgattgttgg gattgggttt tggatgctgc tccgaaaggt
     8101 gggccacagc ttttgcgatg gtggtgccca ctatgcggtt gctgcaggaa ctacagctga
     8161 atgttcgctt tattgtctgc agctcctggg gctggacgaa ggccacgccc tcacccggtg
     8221 cggccatctc ctgcggcaaa acgtatgtgg gattcctgtc caagtagctc tcgatgccca
     8281 ccataatgcg ctccagcagg ttggccaggt gcgggtctag ctgcggcgga ttcgtcgtct
     8341 ggttcactga gtttagggtc tgcagaaaga agtgtgcctc catggtaagc ggattcatct
     8401 gcacgatcag cgggctttag tctaaaaatt cgaatgatac aaactcgtac tcacgtaaag
     8461 cagtttgttt ggcatcatct cggccatcgt cttattcttc ttcgtggcct ttgttaccgg
     8521 agcggctatg ttgggcgcgg tggagcccgg aagctgaaag gttaatcgaa tgcgttaact
     8581 tttctgcaac tcgaaagttt gccgcctttg ttcgactgcc aataactgtt gattcgaaaa
     8641 ttcgaatcga agcggttgaa tttcgtaggg tggccaacta caccaaagtt cgccggcggt
     8701 gtatgcatgt gagtgtgagt gtgacagtgg gtgtacgtgt gctttaagcg acacaatcgc
     8761 accgcgatgt acatattttt gtagaaatgt gtgtgcattt ttcgtccgga tcacggctcc
     8821 ataaaataaa tcatatcaca gggctcaggg atttgtgagt agaagttccc gccacttaaa
     8881 cgtcacttgt gaaatcgcca tcgacggtca acttttactc acactggtct tccgcggacg
     8941 gaccgtctca aagtactgcc tttctgcgtt ggaaaacatc gcctttttcg tccaaaagga
     9001 gtccccaggt tcgatccgca tggcgttgtg cgtgcgtgcc tttcttttca aatgattacg
     9061 gctattaact tgggggcgtt aagttggaaa cacgtaaatt gcagactgcg attagagtga
     9121 ccatgagtag gagttcaaaa tctcctgaca tcattttctt aaaacctgct ttgtttttta
     9181 catttctatt taatataact cctatttgaa taaaaaaaca aaacaagttt agatgttaag
     9241 atattaacta catcctttgc tccaaaggga gaggggaagt tatggagtta attaatttgc
     9301 tgttggaaat caatatggag tcagaaatat aatgatttac taaaccttat tgaatcggta
     9361 acgatgcgaa tttatattaa aatagctttt atgaaacatt caacaaaaat attattaatg
     9421 ttggcccact ttagcaaccg gttaggtcta ccggttgggc aagcaaagat tcacgccctg
     9481 gttcgagtcc caactagtcc tgcaaaatac cgcagcaagt tttagagaga ccaagtgcca
     9541 ttacctctcc cacttcagtt atcggttatg cggcgtttaa gtcgacagct tgccgtctct
     9601 agctccggtg cctatataaa gcagcccgct ttccacattt catattcgtt ttacgtttgt
     9661 caagcctcat agccggcagt tcgaacgtat acgctctctg agtcagacct cgaaatcgta
     9721 gctctacaca attctgtgaa ttttccttgt cgcgtgtgaa acacttccaa taaaaactcc
     9781 tgcaggtgag tactttaaaa aaaaatctag tgaaataatg ctgaaaagaa atttgtgtgg
     9841 gcaaaattca atgggcaaaa acgcgatgcg gctttttctc aaaatggcgg ccggcctgcg
     9901 ttttttcctc aaaagtgatg acgtcatgcc tgtttttttt tttttgttcg caatgaggaa
     9961 tggctcttaa aatctactag ataaaaaaaa tattcattat ttctatgctg ctggaacgct
    10021 tcattaatct taaaaattct aaattcggtt accatgatac ttcgacgcat aactgtagat
    10081 tttggataga attaaagaga aaatggcgag agagtaaaat tccggcgtcg gcaaagtaga
    10141 gcaaaaaaat cagtatacca tttagctacc tctctcactc gcacgcagtg ccggctcaag
    10201 ttgggcgcgg ctctgcaatt atcgattttc ttggggtgtg taactaatca tccgttttcc
    10261 cttcctcctc atccacagcg tgaaggtacc cgcccgggga tcagatccgc ggccgccacc
    10321 atgtacccct acgacgtgcc cgactacgcg atggatagca aaatagaacc tgcagatgcc
    10381 ataagcaacc agaaagctgg tcttacttcg ggcaaaccta aaaaagacaa gtcagtcaag
    10441 gggacgtcgc aagcggttgt ttcagacacc aagaaaatgg aggccagaaa gtcctcctcg
    10501 gacacggtga tcgagccatt gatcaagcag caggcgccac tgcactcgtc aaagaccacg
    10561 ccaacgactt tgacggcgaa cttcgtgcaa aacattcgat tcgtccgtaa aaatgtggaa
    10621 cagtcaggca ggaacaccaa tccactgcag ttcgttgaac tcgagacaga gtttcctcgt
    10681 gactatgacg acaacatcga gatgctttcc cgcgaggcgg agcatctgga ggagcagttc
    10741 cgcacgccta cgcgctccaa tgctacggat gccacttccc accatgtggc cggaatcatc
    10801 gacaacatta taaccgaagc gtcacggagt cttacaatcg aaaagactga agacgatgtg
    10861 ccattgaagt cttctaccaa gcactcaagc ggcgtaaaac gagttggctt tcaagtagag
    10921 gataaggacg ataccgaagt tcagagcgaa aagcaggcaa agagcttcga cgacataacc
    10981 aaaaaagcgg agagctccga ggctagcgca gaggaagctg ctgtcaccgg gtcctcgaca
    11041 gatgcatctg catctcccct gccctccacg tcgctggtct ccaccacatc ttcagctaca
    11101 tcgataacca agagtaagag cgatgaggac gatgatccgg tggccatgtc gccgtgcgga
    11161 cgattcttta agtacgataa ggaagtcgga cgcggttcct ttaaaactgt ctatcgtggc
    11221 ctggacacgt tgactggtgt tccagttgcc tggtgcgagt tattggacaa gcaagtaaaa
    11281 aaaagtgaac gtactagatt tcgcgaggag gcagacatgc tgaagaaact tcagcaccca
    11341 aacatagtgc ggttttatac atattgggag tttcctattg gccgcaagaa gaatattgta
    11401 ctagtcaccg aactaatgtt atctggaact ttgaaatcct atttgaaacg attcaaaaaa
    11461 atacacccaa aggtattgaa gtcatggtgt cgccagatac taaaaggcct taacttcctg
    11521 catactcgtc aatttcctat aattcatcgt gatttgaaat gtgataatat ttttataact
    11581 ggcaccactg gtagtgttaa aatcggcgac ttgggcctgg caactttgaa aaaccgctcc
    11641 cacgcgaaat ctgtgattgg tacgccggag tttatggccc cggaaatgta cgaggagcac
    11701 tacgacgagt cggtggacgt atatgcattc ggcatgtgca tgttggagat ggccatttcc
    11761 gaatatcctt acagtgagtg caagggtcct gcacagatct acaaaaaggt gatatcgggc
    11821 attaagccag cggcgttggc taaggtggag gatcccaacg tgcgcgacat tatcgaacgg
    11881 tgcattgaac ttaaaaagga ggatcgcccc agctgcaatg aactgctaga atcggagttc
    11941 tttgacgaag atatcggtat acgagtggag ccaactgctt cggagcagtt cctttctgat
    12001 ccgagcatca gcatcatcga gttccgactg cggtttatgg atcccaagaa gcgctcgtcc
    12061 cgtcataagg aaaacgaggc aattcagttt gagtacaaca tcaggcatga cgagtacgaa
    12121 cagattgcgc aagaaatgat gaaggaaaat attatatctg aggacgactc tcgcgctgta
    12181 gctcggcttt taaaagtgca ggtggtgtcg ttgcttaagg aacgtgccca gcgtcaaact
    12241 caaatcaagc tgcaaaacga gaagtctcgt ttggaaaaat tggcattgca aaagcaacgt
    12301 gagagtttgc cgactaacgt cgatgaagac gaggaagagg aggaggagtc cgaggacgag
    12361 gaggatggtg ttaaatggaa tcagcgcctg cagctaagat atgacctgct taacactgat
    12421 tcagaaacaa gcctggctct atccacgaac agcgtcgaac cgcagcagct gtccactcgc
    12481 tcgaacactt cgattccgaa cagtggcatt cagcaacctg ttcaggtccc aggtcaagtg
    12541 cctgtttccc agttgatctc ggtgcaacca caggctattc cgtcaccggc tattcccatg
    12601 caacagaagc cgacggtgca ctacattcaa ccgccacagc tggcctctta tcaaaattcg
    12661 aatgcatcga tgcaggagat gaccaataac caggtcattt cgccgacagg cagtcaacaa
    12721 atgcaacagc aacaacccgt agtggcacca acagtcaatc atcaggtcat gccgcagcag
    12781 caagtgaacc aacaacagca acaaccacaa atgatgcagc aaatacccca gcaagttcaa
    12841 gtgcaacagc cccaaactgt attgcctccg cagccacatg agcagcagcc acagcaacaa
    12901 caacagccac tgcagcagca actgcagcag cttatgcata ctaacgtgca ggctccggat
    12961 ttgactcaac agcaacaaat ggctcagcaa caggcccagc aatattttca gcagcagcag
    13021 caacagcctc agcaggccgt taacatgcaa caagcttatg caatgcagca agctggccaa
    13081 cagcaacagc tttcgcagcc tcttcaaatt cagcaacaga tcttgcagca acaacaagtg
    13141 gccgtttcac atcagcaaca gataatgcag caacaactgg cccagcatca gctgcaacag
    13201 ctgcagcagc agcagcttca acaacagcaa ctgcaacagc aacagcagca gcaaatacaa
    13261 caacagcaac tccaacaaca acaactccag caacagcaat tcgtacagca gtatgcgcag
    13321 gcaatgcctc agcaacaaca tcagcaactc gttacgggat cgcaagtcat ggcaccgcat
    13381 caacatcagc aacctattca gatcccagtg cagatgcaag ttccaccgac gtccgtggct
    13441 cctccaatac aacacactta caatcagcag ggcggacaag taacgctcag tgatgcccaa
    13501 cagcaacagc atcctggatt ctcggccgta ccacaacagg cagcgccatt tatacaacag
    13561 cccacacagc aacccattca gctatcaatg cctttggaac aacaattgca gcaattgctg
    13621 catagccagc cagcccagca acaacaggcc atgagccaac agcagcaaca gtctatggtc
    13681 caacagcagc aacagccctt ggtgcaacag caacaactgc ctttggtaca acagcagccg
    13741 cctttggtcc agcagcagca accgctggtt cagcatcagc agccttcggt ccaacatcag
    13801 caacctttgg tgcaacagcc ccagcaacag caaccacagc cccagaatca gcaaccacag
    13861 ccccagacac agcaaacaca cgtggttcag caacaaccac cacagcagca acctgcagta
    13921 gagcaaatct cacagatttc ttcgcaagtg cctgttcaac aggaaaatct gcagccaata
    13981 caggtcaaca aagatgcgaa tgtcgcaacg gatgcaatgt ccctaaatag cgcccacggt
    14041 gccctcgaac ctgcaccgaa gaccgaaccc cagaactctg ctgatgcgga aaagcagcaa
    14101 aagcagacag gcacgggcac acgttcccag aaaccgaggc gatcaaaccg gagcggcaac
    14161 gaacggattc ccaaacttag tgtaaccagc gtcgacgagg gcagcgtcat aaactgtcac
    14221 atggaaaata aacttaaaac gattacattt aaattcgaca taggagacgt aaatccggtt
    14281 gaaatagcca ataaattgat cgcccaagat ttgctttcta attgtcaaag tacagttttt
    14341 gtggaaatga tcaatgaaat tgttgaccaa gtcaaacaga atcccaatca aatccctata
    14401 ccgacaaatt atcgccgcaa tattgaaaag cgggatgaaa ccgccagcga catcacaaag
    14461 atgttcgagc ccactatcca tggtgttgaa accctgccat ccggtggagg aggcgctgaa
    14521 cagtcaaact gcaacttgac tttagaggcc aggtcccatt tatccaacat accaaatgcg
    14581 aaagaaccac agctgaacgt cagcactccg cccacaacca cttcgactat gtcttcctca
    14641 tcaaccgcat cgagggatgc gcccaatagt agcaatgacg tgaccatcgg ctctggttcc
    14701 gtttctcgaa aaacgagcac ggcctcggag tacacttcgc tatcgattga ctatatgccc
    14761 gacagcaaca ttactccaac tggtccggag ccaccgcttg acgatggcaa ggataaaaaa
    14821 ccttgctccc ttgctatagc cagaatgcaa aagctcttgg agtcggccgg caatggtgct
    14881 cctcgcagcc tgaatttgcc cttgaatcgt cacctgaaaa ttcaagagga cttgaagcat
    14941 actagaagtc ttgacgattt gaccgcagtg aaaataacct ttgacatgcc caacaaggct
    15001 gcattggaca cttcggaaaa cgctcagcag gccacagaag cggaagcgga gaaaccaaaa
    15061 gataaatccg gacaggcagt aggcaaccag ggaactggag cggccaacac tctagagcag
    15121 ttaaaaatag aactggagaa cattactcat gcccacgcgt ttgcctccgc tgtagtggcc
    15181 tcgattaaca acagagctcc tcatcaagct tccccagcaa tgtcctccct aaaggccact
    15241 tctggacaac cacagacaca agagataacc aagccaaata acggagcagg cccttcagta
    15301 ccctcagtgg gtcagaatac tcccactgca gccttaacat ccgccagggg ctcaggctcc
    15361 tccgtctaca actcacgtcg cacatccatc gacaacagcg tgggatccga catgcacctg
    15421 cacactgcca ctaatcttga agggacagtg agcaacccgg atccgatccc gactgaggca
    15481 tcggttggaa ttaccattgc tacgggtcat gagaagcaac tctccaagca gcccagtttg
    15541 gaaaagcctt ctgccacttc cattttaacg aacaacagtg atcctccgca aaggaatccc
    15601 agcaatggat caattaacca aaattcaata gctgatttgg aaaagaaatt ggcagctttg
    15661 cgaaataccg aaaacacaga agagtctgca acagcaacgt tgtcagtggt tcctaaacaa
    15721 gttgaagagg tagtcaatcc aagcgcgcgt aaaatttcgc gttttagtgt cagtcgtgtg
    15781 caggagcaga aaacttcaac cggagttgag gaaccagctc aaggtcaact taagatcgac
    15841 ctccaagtcg ccggccctgg aggccaggtg cagaccaaca actccgttca gaacggcagc
    15901 gtggtcaaca ctccaaccga agtcatcagc tctcctattc aaaatgttcc gctggccatt
    15961 aacggaattc agttgatgta ccaacagccc cagcagatac accagttgcc aggtggaaca
    16021 tcaactacta caagtggagc agggactcag tgcattgtag tgccgcaagt ggtcaatcaa
    16081 aatggcaccc acgtgcaaca gccgtctaac ctacagccac agcagcagag cgtacatcca
    16141 aatatgactc aacagccaca gcagacgcca cttaatgggc acccatctat ggttaacact
    16201 cttcagcagc agccaccaca acaatcctta ccgatgcaga cgattcagtc gcagcagcag
    16261 cagcataacc aaatgcctat catttcgcaa caacagcagc agcagatcct aatgcagcag
    16321 caacagcaac agggatccca gcaaggatca cagcaattta atttgcctgg cacacaacag
    16381 actcatcctc agcaccagtt catccagtcg cagcccaacc aacttcactc gcttccgccc
    16441 caggtagtga gcatggctca gggaaatctg ccgcatcagc ttccacccat gcagactatg
    16501 ggagcccaac agcaaatgat ctcgcaacaa cagcatcagt tgcagatgca gtcgcatatg
    16561 caccaacaaa acgtgcccgg aatgtacaac cagcagactg gagctcgtgt ggcagcaccc
    16621 caaaatttcg caggtgcagt tccaaatcat ttgttgcagc agtccccttt gatggcatcc
    16681 cagcagccag cacagcccat gcaacacgtt atgcaaccga ttttcaatgc aacgtccggt
    16741 gaagttaccg aagagcctgt ctctttagca gctacgcatc cgcatctgct accgagcgat
    16801 attcaatccg atataaagca caatttagac tcattagtga accaattgtg caatacgcgc
    16861 ttgggtacta accagcacca acggctgttg ctgttgcgtc agagacaact catcgaagag
    16921 gacgagctgc gtcttaaaca ttatgtggaa tacgaaaagt tccaaaaggc actgcgccaa
    16981 tctattagca caaacgttcc agctaatgca gcttactatt ctgcggctgc cgcacagctt
    17041 ccaactaatt tggcagcccc agcagctccg tcctcatcga atccgacgtc cactagctcc
    17101 gcaaacacat aac
//
