LOCUS       sequence-39549-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  TITLE     Boussiotis Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Smad3-shRNA
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(199..217)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(249..269)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     467..486
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagcagagct ggttagtgaa ccgtcagatc cgctagcgct accggactca gatccaccgg
       61 tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca
      121 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      181 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
      241 gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg
      301 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      361 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct
      421 tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga
      481 agaagactat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
      541 agggcgagat ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
      601 agtccatcta catggccaag aagncccgtg cagctgcccg gctactacta cgtggactcc
      661 aagctggacn tcacct
//
