LOCUS       sequence-395518-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Murawala P
  TITLE     Murawala Lab Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Twist3cterm-GFPnls-T2A-ER-Cre-ER
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..38
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     30..47
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1308..1361
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1362..1364,1365..1367,1368..2078)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1362..1364,1365..1367,1368..2078)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1668..1687)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2015..2036
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2103..2123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2127..2147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2151..2171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2190..2243
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3192..4214
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             4227..5162
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4275..5168
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5252..5271)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5317..5372
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5371..5390)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(5702..5720)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5703..5720)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5738..5754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5738..5754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5751..5773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5762..5778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5786..5792,5793..5810,5811..5816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5831..5852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5969..5986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6140..6728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6220..6239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6899..7690,7691..7759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7522..7541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7760..7864)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7942..8397)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8079..8100)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8291..8310
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
       61 actcactata gacgcgtcac tcaaccttgt tcgcaggtaa catacaacaa gaagttgttg
      121 tcattattat tattaatgtc atcaacacac cggtgcatat gtaggaggcc caatgctttc
      181 aggacagcaa gctaatattg tctcggtttt gcgagtttcc cagagtggcg gcgtgcgttt
      241 cccatagaga gctcctaatg cccgcggccg ggctttgatg aggtcccagg agggaggcct
      301 cggcatcgcc ctccatcccc tgccgccgtg cactttcccc gctctacctc cgacatccgg
      361 gcagcagagc atctgtgcgc cttcaagggc tgcagcccat gcaatctgga tcccataaac
      421 ggcaggacct tcggtgacgg attggtttcg acgctgcctc cgccccagcc caccctcctg
      481 cacaccaggc caccccctca gccccacccg tttacccttt gctacccaat caggcagagg
      541 catttcacaa ttccttcccg ggttcctcct ctccttcctg aaagaagagg ggaagagaga
      601 aaaagccgaa gtggtgtgaa gcattttgga agtcttagat gctgtggagg tgagcggtgc
      661 tcagcctgtg tgcctgggga catatggtgc cacctattct tcggggacgc cctgtgcctt
      721 cctggaacgc cgccttactg gattccacca gtggtttcct acggtcaaac tcttcattgc
      781 agtggatcca acacgcgcag gctgcaaagt tcttcacaat ggaaatgaag gaggagagct
      841 catgcccgga gtctcccgaa ggcagcctgg agaccagcga ggaagaggcc gaccgactgc
      901 acaagaaatg catgcgcaag cgcagccagc tgggcaaggc ccacgatgac tgtggttcga
      961 catccccatc cggcaaaagg aacaagcgca gcccattgcc ccagtccttc gaagacgttc
     1021 acacgcagag ggtcattgcc aatgtgcgcg agcgccagcg gacccagtcg ctgaatgacg
     1081 cctttgcaga gttgcgcaaa atcatcccta cgctgccctc cgacaagctg agcaagatcc
     1141 agacgctaaa gctggcctca cgctatatag actttttgta ccaggtgctg caaagtgatg
     1201 agctggacca caagatcgcc agctgcaact acctggccca cgagcggctt agctatgcct
     1261 tctcggtttg gcgtatggaa ggggcctggt ccatgtctgc aactcacgag ggcagaggaa
     1321 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatggtgagc aagggcgagg
     1381 agctgttcac cggggtggta cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1441 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1501 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1561 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1621 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1681 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1741 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctagag tacaactaca
     1801 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1861 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1921 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1981 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2041 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctg
     2101 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     2161 agagaaaggt aggatccacc ggatctagag agggcagagg aagtcttcta acatgcggtg
     2221 acgtggagga gaatcccggc cctatggccg gcgatatgag agccgctaac ctgtggccct
     2281 ccccactgat gatcaagaga agcaagaaga actccctggc cctgagcctg accgctgatc
     2341 agatggtgtc cgccctgctg gacgccgagc cccctatcct gtacagcgag tacgacccca
     2401 ccaggccctt cagcgaggcc agcatgatgg gactgctgac aaacctggcc gatcgcgaac
     2461 tggtgcatat gatcaattgg gccaagcgcg tgcccggatt cgtggacctg acactgcacg
     2521 accaggtgca cctgctggaa tgcgcttggc tggaaattct gatgatcggc ctcgtgtggc
     2581 ggagcatgga acatcctgtg aagctgctgt tcgcccccaa tctgctgctg gaccgcaacc
     2641 agggcaaatg cgtggaaggg atggtcgaaa tctttgatat gctgctcgcc accagcagca
     2701 ggttcagaat gatgaacctc cagggcgaag agttcgtctg cctgaagtcc atcatcctgc
     2761 tgaacagcgg cgtgtacacc ttcctgtcct ctaccctgaa gtccctcgag gaaaaggatc
     2821 acatccacag ggtgctggat aagatcaccg ataccctgat ccatctcatg gctaaggccg
     2881 gactcacact ccagcagcag catcagagac tcgctcagct gctgctgatc ctgagccaca
     2941 tccgccacat gtccaacaag ggaatggaac acctctactc tatgaagtgt aaaaacgtcg
     3001 tccccctgta cgatctgctg ctcgaggccg ccgatgccca cagactgcac gcccctacaa
     3061 gcagaggcgg cgctagcgtg gaagagacag accagtccca cctggccacc gccggcagca
     3121 caagcagcca cagcctgcag aagtactata tcaccggcga ggccgagggc ttccccgcta
     3181 cagctgtcga caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
     3241 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
     3301 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga
     3361 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
     3421 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
     3481 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
     3541 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
     3601 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
     3661 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
     3721 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
     3781 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
     3841 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
     3901 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
     3961 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
     4021 ccggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
     4081 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     4141 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
     4201 tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac ctttggccaa
     4261 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
     4321 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
     4381 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     4441 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     4501 atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     4561 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     4621 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     4681 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     4741 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     4801 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     4861 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     4921 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     4981 tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat gcgcccacta
     5041 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     5101 cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca
     5161 cagcttaaga tatcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     5221 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     5281 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     5341 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     5401 atttaaaaca tcagaatgag catttggttt agagtttggc aacatatgcc catatgctgg
     5461 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     5521 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     5581 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     5641 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatcta
     5701 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5761 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5821 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5941 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6001 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6061 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6541 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6781 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6841 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7021 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7201 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7741 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7861 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     7921 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7981 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     8041 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     8101 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     8161 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     8221 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8281 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8341 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     8401 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8461 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttggg
//
