LOCUS       sequence-395520-        8044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for LFNG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Murawala P
  TITLE     Murawala Lab Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCS:GFPnls-T2a-ER-Cre-ER
FEATURES             Location/Qualifiers
     source          1..8044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(677..688,689..876)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     intron          999..1131
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1114..1133
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1228..1247
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1289..1298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1295..1297,1298..1300,1301..2011)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1295..1297,1298..1300,1301..2011)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1601..1620)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1948..1969
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2036..2056
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2060..2080
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2084..2104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2123..2176
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3128..4150
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             4163..5098
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4211..5104
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5188..5207)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5253..5308
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5307..5326)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(5657..5794,5795..5806)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5833..5853)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5833..5851)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5895..5911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5895..5911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5908..5930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5919..5935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5943..5949,5950..5967,5968..5973))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5988..6009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6126..6143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6297..6885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6377..6396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7056..7847,7848..7916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7679..7698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7917..8021)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattggcaga ggtgtaaagt acttgagtaa ttttacttga ttactgtact
      721 taagtattat ttttggggat ttttacttta cttgagtaca attaaaaatc aatactttta
      781 cttttactta attacatttt tttagaaaaa aaagtacttt ttactcctta caattttatt
      841 tacagtcaaa aagtacttat tttttggaga tcacttgggc ccggctcgag catgcacgcg
      901 ttagcggccg ctaggtacca tgaattcgct gagggcgttt aaaatcctca gcaattcgaa
      961 ttttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
     1021 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
     1081 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
     1141 cccagttcaa ttacagctct taaaattccg ccactagagc ctctgctaac catgttcatg
     1201 ccttcttctc tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1261 ttggcaaaga attattccgc tagcgctcgc caccatggtg agcaagggcg aggagctgtt
     1321 caccggggtg gtacccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1381 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1441 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1501 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1561 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1621 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1681 cgacttcaag gaggacggca acatcctggg gcacaagcta gagtacaact acaacagcca
     1741 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1801 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1861 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1921 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1981 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctgatccaaa
     2041 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
     2101 ggtaggatcc accggatcta gagagggcag aggaagtctt ctaacatgcg gtgacgtgga
     2161 ggagaatccc ggccctgtca tggccggcga tatgagagcc gctaacctgt ggccctcccc
     2221 actgatgatc aagagaagca agaagaactc cctggccctg agcctgaccg ctgatcagat
     2281 ggtgtccgcc ctgctggacg ccgagccccc tatcctgtac agcgagtacg accccaccag
     2341 gcccttcagc gaggccagca tgatgggact gctgacaaac ctggccgatc gcgaactggt
     2401 gcatatgatc aattgggcca agcgcgtgcc cggattcgtg gacctgacac tgcacgacca
     2461 ggtgcacctg ctggaatgcg cttggctgga aattctgatg atcggcctcg tgtggcggag
     2521 catggaacat cctgtgaagc tgctgttcgc ccccaatctg ctgctggacc gcaaccaggg
     2581 caaatgcgtg gaagggatgg tcgaaatctt tgatatgctg ctcgccacca gcagcaggtt
     2641 cagaatgatg aacctccagg gcgaagagtt cgtctgcctg aagtccatca tcctgctgaa
     2701 cagcggcgtg tacaccttcc tgtcctctac cctgaagtcc ctcgaggaaa aggatcacat
     2761 ccacagggtg ctggataaga tcaccgatac cctgatccat ctcatggcta aggccggact
     2821 cacactccag cagcagcatc agagactcgc tcagctgctg ctgatcctga gccacatccg
     2881 ccacatgtcc aacaagggaa tggaacacct ctactctatg aagtgtaaaa acgtcgtccc
     2941 cctgtacgat ctgctgctcg aggccgccga tgcccacaga ctgcacgccc ctacaagcag
     3001 aggcggcgct agcgtggaag agacagacca gtcccacctg gccaccgccg gcagcacaag
     3061 cagccacagc ctgcagaagt actatatcac cggcgaggcc gagggcttcc ccgctacagc
     3121 tgtcgacaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag
     3181 tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca
     3241 tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa
     3301 ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc
     3361 gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg
     3421 tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat
     3481 ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac
     3541 tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa
     3601 tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat
     3661 cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac
     3721 gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact
     3781 ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg
     3841 ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc
     3901 cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg atgactccgg
     3961 tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc
     4021 ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat
     4081 tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga
     4141 agatggcgat ctcgagccat ctgctggaga catgagagct gccaaccttt ggccaagccc
     4201 gctcatgatc aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat
     4261 ggtcagtgcc ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag
     4321 acccttcagt gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt
     4381 tcacatgatc aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca
     4441 ggtccacctt ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc
     4501 catggagcac ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg
     4561 aaaatgtgta gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt
     4621 ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa
     4681 ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat
     4741 ccaccgagtc ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct
     4801 gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag
     4861 gcacatgagt aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc
     4921 cctctatgac ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg
     4981 tggaggggca tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc
     5041 atcgcattcc ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc
     5101 ttaagatatc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5161 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5221 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5281 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5341 aaaacatcag aatgagcatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5401 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
     5641 ccagcggccg gccgctaata ctcaagtaca attttaatgg agtacttttt tacttttact
     5701 caagtaagat tctagccaga tacttttact tttaattgag taaaattttc cctaagtact
     5761 tgtactttca cttgagtaaa atttttgagt actttttaca cctctgccgc ggtggagctc
     5821 cagcttttgt tccctttagt gagggttaat tgcgcgcatt accctgttat ccctacgcgc
     5881 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8041 ccac
//
