LOCUS       sequence-395521-       10389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Murawala P
  TITLE     Murawala Lab Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LFNG:GFPnls-T2a-ER-Cre-ER
FEATURES             Location/Qualifiers
     source          1..10389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(677..688,689..876)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     intron          3344..3476
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3459..3478
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3573..3592
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3634..3643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3640..3642,3643..3645,3646..4356)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3640..3642,3643..3645,3646..4356)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3946..3965)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4293..4314
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4381..4401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4405..4425
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4429..4449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4468..4521
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5473..6495
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6508..7443
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6556..7449
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(7533..7552)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7598..7653
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7652..7671)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(8002..8139,8140..8151)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8178..8198)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8178..8196)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8240..8256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8240..8256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8253..8275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8264..8280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8288..8294,8295..8312,8313..8318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8333..8354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8471..8488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8642..9230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8722..8741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9401..10192,10193..10261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10024..10043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10262..10366)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattggcaga ggtgtaaagt acttgagtaa ttttacttga ttactgtact
      721 taagtattat ttttggggat ttttacttta cttgagtaca attaaaaatc aatactttta
      781 cttttactta attacatttt tttagaaaaa aaagtacttt ttactcctta caattttatt
      841 tacagtcaaa aagtacttat tttttggaga tcacttgggc ccggctcgag catgcacgcg
      901 ttagcggccg cgaattcata ggtgacaaca gagagtcatg ctatagtcta ttctgtgtct
      961 gtgtttgtgt gagagagaga cagagagaga cagagagaca gagagacaga gacagagaga
     1021 cacagagaaa gtagaatgga ggctgccaag tggtccagga gaggggtctc aaggaggagc
     1081 cttagaccta aaacactcgg aagacacata gatagttttt ggtgggaatg gattagcccc
     1141 tgaggctgag gctggaactg gggtgagttt atttggagag cagtccagca gatagcaggt
     1201 aggacttttt ccttgtcctt gagggaccag gctctggcag gaggaggagg ggtttatagc
     1261 aggaggtgac gccatgggag ctttgtcact cgggcacagc cagcaccatc tgcttaggca
     1321 tctgcctacc agaggtggtg cctcctaatt acctgggggg aggggtcagg aagaagcctg
     1381 ggaaccacca tatcccactc cctcccacca cagaggggtt gcagtgtccc acagggccct
     1441 cagagcaaat ggaccagaga tccactcctg ggtggggtgg gaggctgggg ggtggggtgg
     1501 ggtggggaga gcctggctac acatcaattc aaagtcattt tattttatat ttgtgatagg
     1561 gtctcatgta gctcaggctg gactcaaact cactatttgc caaggaatat ttggtgcaga
     1621 gatgaaccga ggcgtcatac atgctaggca aacactttac caactttgcc agatgaggta
     1681 catttcaggc gctcttttac acggcagcag gggctgaccc gatattcact ctgtaactca
     1741 ggctgccttt aaatgttcag caatcgtcca agcctgctac atgggctatc atgtctgtct
     1801 tcattttgaa aagaattttg ttgaaagcag gtgtgcgtat gtgtatgtgc gtgcgtgcgt
     1861 gcgtgtgtat aaatgtgtgt tgctcaggcc aagttgcacg tgtggaggtc agagggcaac
     1921 atattgttgg ttccttctga ccatgtgggt cctgggaaat aaactcaaat ctgcctaggt
     1981 gacagttgcg tttgcccact gaaccatctg gccagcccct gcagtaagtc tttacctgat
     2041 ccagcctgat gccttctttt aacctccaag agagggacgg acattaagaa gagtgtagta
     2101 gagaaataat tagaaatgat actccggtct aaaatataaa cgctagggag tcggcgacag
     2161 aaggaacagc agtttatccg tggtggcagg gtgggctcag acttcctttg aagggggacg
     2221 cggaaactga agccaaccag acccaggggc agccaggcgg agagccgccc ccagctgcgc
     2281 tcccggaaga tgcagcaggc tcgggctcgg accctgctcc cactccaggg gacccttggc
     2341 catttccgga cccgtagcct ctcaccctac cattaggcgc acattcctgc cgcccccgct
     2401 cgtgctaggc gcaggcaagg cccccgcccg tgggaagggg gccgtgtgcc ggggaggcgg
     2461 gtctgggatg gcgcacagct gggtcacatc tggtaggagt gcggaggaac caaatggaac
     2521 agaaggtagc ctttcatcct tcgaggaaag aggctgcttg agtgtctcct cgctacgggg
     2581 tgcctgaggt ttttggggac caaccctccc catttaggaa catgatgcca agacaggcct
     2641 gcctggggga ggagggaagg atggaccgac ggagggacgt agaattttgt cactgatacc
     2701 cttttgcctg aacttcaggt ccactcctaa gacataagta tcagtaaggg cagctcaaaa
     2761 ttaatcgctc aggacacact gcaggctacc actttggctc agtcagcacc gttccccagg
     2821 gaaggaaacc cgctcgggga caggaaatgc acagcgagaa gccaaggatg tcgcccgcgg
     2881 aggagcactg gccctatcac ccaggtccca gcttcggggg cgcgtcctcc acgtgcagcc
     2941 gtggcgggcg ttggtcaggc tgggtgtctc ccagagcagc tccggagggt gactcggcgc
     3001 aggcgggaca ggctcgtgca caaagggggc gggtgccgtg ggtaccccag ctgggtggtt
     3061 cccagcccct tgtccgtatc ccacgtggga aggagagatc tccctaaagg ttacgcccca
     3121 aggctagcag ccaattagcg cgtggaggcg tggcgggcag gggaggttta agagttcctg
     3181 aagcgagagg cgcgcagctg gaccgggagc ctcgggcagc cggaccagtt ggcactggga
     3241 tagatcttaa gcttggtacc gagctcgaat tcgctgaggg cgtttaaaat cctcagcaat
     3301 tcgaatttta agctgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
     3361 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
     3421 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
     3481 ccactcccag ttcaattaca gctcttaaaa ttccgccact agagcctctg ctaaccatgt
     3541 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     3601 tcattttggc aaagaattat tccgctagcg ctcgccacca tggtgagcaa gggcgaggag
     3661 ctgttcaccg gggtggtacc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3721 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3781 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3841 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3901 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3961 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4021 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctagagta caactacaac
     4081 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4141 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4201 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4261 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4321 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctgat
     4381 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag
     4441 agaaaggtag gatccaccgg atctagagag ggcagaggaa gtcttctaac atgcggtgac
     4501 gtggaggaga atcccggccc tgtcatggcc ggcgatatga gagccgctaa cctgtggccc
     4561 tccccactga tgatcaagag aagcaagaag aactccctgg ccctgagcct gaccgctgat
     4621 cagatggtgt ccgccctgct ggacgccgag ccccctatcc tgtacagcga gtacgacccc
     4681 accaggccct tcagcgaggc cagcatgatg ggactgctga caaacctggc cgatcgcgaa
     4741 ctggtgcata tgatcaattg ggccaagcgc gtgcccggat tcgtggacct gacactgcac
     4801 gaccaggtgc acctgctgga atgcgcttgg ctggaaattc tgatgatcgg cctcgtgtgg
     4861 cggagcatgg aacatcctgt gaagctgctg ttcgccccca atctgctgct ggaccgcaac
     4921 cagggcaaat gcgtggaagg gatggtcgaa atctttgata tgctgctcgc caccagcagc
     4981 aggttcagaa tgatgaacct ccagggcgaa gagttcgtct gcctgaagtc catcatcctg
     5041 ctgaacagcg gcgtgtacac cttcctgtcc tctaccctga agtccctcga ggaaaaggat
     5101 cacatccaca gggtgctgga taagatcacc gataccctga tccatctcat ggctaaggcc
     5161 ggactcacac tccagcagca gcatcagaga ctcgctcagc tgctgctgat cctgagccac
     5221 atccgccaca tgtccaacaa gggaatggaa cacctctact ctatgaagtg taaaaacgtc
     5281 gtccccctgt acgatctgct gctcgaggcc gccgatgccc acagactgca cgcccctaca
     5341 agcagaggcg gcgctagcgt ggaagagaca gaccagtccc acctggccac cgccggcagc
     5401 acaagcagcc acagcctgca gaagtactat atcaccggcg aggccgaggg cttccccgct
     5461 acagctgtcg acaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca
     5521 acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct
     5581 gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg
     5641 aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt
     5701 caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt
     5761 catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg
     5821 cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa
     5881 cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata
     5941 cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc
     6001 aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc
     6061 agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact
     6121 aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg
     6181 ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact
     6241 cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac
     6301 tccggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat
     6361 atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta
     6421 aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg
     6481 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca
     6541 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
     6601 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
     6661 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
     6721 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
     6781 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
     6841 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
     6901 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
     6961 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
     7021 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
     7081 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
     7141 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
     7201 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
     7261 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
     7321 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
     7381 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
     7441 acagcttaag atatcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     7501 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     7561 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     7621 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     7681 catttaaaac atcagaatga gcatttggtt tagagtttgg caacatatgc ccatatgctg
     7741 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     7801 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     7861 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     7921 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc
     7981 ctcgaccagc ggccggccgc taatactcaa gtacaatttt aatggagtac ttttttactt
     8041 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     8101 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gccgcggtgg
     8161 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcattaccct gttatcccta
     8221 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8401 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10381 aagtgccac
//
