LOCUS       sequence-395618-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Type 0 terminator parts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pMetBP-T_cRed_BsaI
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(170..264)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(285..944)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     859..878
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1106..1124
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1128..1171)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      7282..7291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      9167..9214
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      9243..9300
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(9324..9341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9495..10083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9575..9594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
       61 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      121 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
      181 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      241 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      301 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      361 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      421 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      481 ctggtgaaac tcacccaggg attggctgac acgaaaaaca tattctcaat aaacccttta
      541 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      601 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      661 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      721 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      781 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      841 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      901 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      961 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1021 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     1081 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     1141 aaaatcctta gctttcgcta aggatgattt cgacttggtc tcgtcgatga gaccgcagcg
     1201 gtctgccaat ccgacggagg tttatgaatt ttccaccttt tccacaagct caactagtat
     1261 taacgatgtg gatttagcaa aaaaaacctg taaccctaaa tgtaaaataa cgggtaagcc
     1321 tgccaaccat gttatggcag attaagcgtc tttttgaagg gcaccgcatc tttcgcgttg
     1381 ccgtaaatgt atccgtttag caataaggaa attcttaatg aataatccgt cgttactcaa
     1441 tcatgcggtc gaaacgatgg cagttggctc gaaaagtttt gcgacagcct caaagttatt
     1501 tgatgcaaaa acccggcgca gcgtactgat gctctacgcc tggtgccgcc attgtgacga
     1561 tgttattgac gatcagacgc tgggctttca ggcccggcag cctgccttac aaacgcccga
     1621 acaacgtctg atgcaacttg agatgaaaac gcgccaggcc tatgcaggat cgcagatgca
     1681 cgaaccggcg tttgcggctt ttcgggaagt ggctatggct catgatatcg ccccggctta
     1741 cgcgtttgat catctggaag gcttcgccat ggatgtacgc gaagcgcaat acagccaact
     1801 ggatgatacg ctgcgctatt gctatcacgt tgcaggcgtt gtcggcttga tgatggcgca
     1861 aatcatgggc gtgcgggata acgccacgct ggaccgcgcc tgtgaccttg ggctggcatt
     1921 tcagttgacc aatattgctc gcgatattgt ggacgatgcg catgcgggcc gctgttatct
     1981 gccggcaagc tggctggagc atgaaggtct gaacaaagag aattatgcgg cacctgaaaa
     2041 ccgtcaggcg ctgagccgta tcgcccgtcg tttggtgcag gaagcagaac cttactattt
     2101 gtctgccaca gccggcctgg cagggttgcc cctgcgttcc gcctgggcaa tcgctacggc
     2161 gaagcaggtt taccggaaaa taggtgtcaa agttgaacag gccggtcagc aagcctggga
     2221 tcagcggcag tcaacgacca cgcccgaaaa attaacgctg ctgctggccg cctctggtca
     2281 ggcccttact tcccggatgc gggctcatcc tccccgccct gcgcatctct ggcagcgccc
     2341 gctctaggct tactataagg atgtttgcaa tgaaaccaac tacggtaatt ggtgcaggct
     2401 tcggtggcct ggcactggca attcgtctac aagctgcggg gatccccgtc ttactgcttg
     2461 aacaacgtga taaacccggc ggtcgggctt atgtctacga ggatcagggg tttacctttg
     2521 atgcaggccc gacggttatc accgatccca gtgccattga agaactgttt gcactggcag
     2581 gaaaacagtt aaaagagtat gtcgaactgc tgccggttac gccgttttac cgcctgtgtt
     2641 gggagtcagg gaaggtcttt aattacgata acgatcaaac ccggctcgaa gcgcagattc
     2701 agcagtttaa tccccgcgat gtcgaaggtt atcgtcagtt tctggactat tcacgcgcgg
     2761 tgtttaaaga aggctatcta aagctcggta ctgtcccttt tttatcgttc agagacatgc
     2821 ttcgcgccgc acctcaactg gcgaaactgc aggcatggag aagcgtttac agtaaggttg
     2881 ccagttacat cgaagatgaa catctgcgcc aggcgttttc tttccactcg ctgttggtgg
     2941 gcggcaatcc cttcgccacc tcatccattt atacgttgat acacgcgctg gagcgtgagt
     3001 ggggcgtctg gtttccgcgt ggcggcaccg gcgcattagt tcaggggatg ataaagctgt
     3061 ttcaggatct gggtggcgaa gtcgtgttaa acgccagagt cagccatatg gaaacgacag
     3121 gaaacaagat tgaagccgtg catttagagg acggtcgcag gttcctgacg caagccgtcg
     3181 cgtcaaatgc agatgtggtt catacctatc gcgacctgtt aagccagcac cctgccgcgg
     3241 ttaagcagtc caacaaactg cagactaagc gcatgagtaa ctctctgttt gtgctctatt
     3301 ttggtttgaa tcaccatcat gatcagctcg cgcatcacac ggtttgtttc ggcccgcgtt
     3361 accgcgagct gattgacgaa atttttaatc atgatggcct cgcagaggac ttctcacttt
     3421 atctgcacgc gccctgtgtc acggattcgt cactggcgcc tgaaggttgc ggcagttact
     3481 atgtgttggc gccggtgccg catttaggca ccgcgaacct cgactggacg gttgaggggc
     3541 caaaactacg cgaccgtatt tttgcgtacc ttgagcagca ttacatgcct ggcttacgga
     3601 gtcagctggt cacgcaccgg atgtttacgc cgtttgattt tcgcgaccag cttaatgcct
     3661 atcatggctc agccttttct gtggagcccg ttcttaccca gagcgcctgg tttcggccgc
     3721 ataaccgcga taaaaccatt actaatctct acctggtcgg cgcaggcacg catcccggcg
     3781 caggcattcc tggcgtcatc ggctcggcaa aagcgacagc aggtttgatg ctggaggatc
     3841 tgatatgagc ttttacccag gagacagaga atgcaaccgc attatgatct gattctcgtg
     3901 ggggctggac tcgcgaatgg ccttatcgcc ctgcgacttc agcagcagca acctgatatg
     3961 cgtattttgc ttatcgacgc cgcaccccag gcgggcggga atcatacgtg gtcatttcac
     4021 cacgatgatt tgactgagag ccaacatcgt tggatagctc cgctggtggt tcatcactgg
     4081 cccgactatc aggtacgctt tcccacacgc cgtcgtaagc tgaacagcgg ctacttttgt
     4141 attacttctc agcgtttcgc tgaggtttta cagcgacagt ttggcccgca cttgtggatg
     4201 gataccgcgg tcgcagaggt taatgcggaa tctgttcggt tgaaaaaggg tcaggttatc
     4261 ggtgcccgcg cggtgattga cgggcggggt tatgcggcaa attcagcact gagcgtgggc
     4321 ttccaggcgt ttattggcca ggaatggcga ttgagtcacc cgcatggttt atcatctccg
     4381 attatcatgg atgccacggt cgatcagcaa aatggttatc gcttcgtgta cagcctgccg
     4441 ctctcgccga ccagattgtt aattgaagat acgcactata ttgataatgc gacattagat
     4501 cctgaatgcg cgcggcaaaa tatttgcgac tatgccgcgc aacagggttg gcagcttcag
     4561 acactgctgc gagaagaaca gggcgcctta cccattactc tgtcgggcaa tgccgacgca
     4621 ttctggcagc agcgccccct ggcctgtagt ggattacgtg ccggtctgtt ccatcctacc
     4681 accggctatt cactgccgct ggcggttgcc gtggccgacc gcctgagtgc acttgatgtc
     4741 tttacgtcgg cctcaattca ccatgccatt acgcattttg cccgcgagcg ctggcagcag
     4801 cagggctttt tccgcatgct gaatcgcatg ctgtttttag ccggacccgc cgattcacgc
     4861 tggcgggtta tgcagcgttt ttatggttta cctgaagatt taattgcccg tttttatgcg
     4921 ggaaaactca cgctgaccga tcggctacgt attctgagcg gcaagccgcc tgttccggta
     4981 ttagcagcat tgcaagccat tatgacgact catcgttaag cttcagagga ggaattaagt
     5041 aatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
     5101 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
     5161 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
     5221 cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg atgtggggca
     5281 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
     5341 gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
     5401 cgggcattca tcaacctcca ttagtgccgg aattggtatt gcggttgctg ccgaaaaaga
     5461 aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg caggcatggc
     5521 gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtga ttctcaacga
     5581 caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaac aaccatctgg cacagctgct
     5641 ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct ctggcgtgcc
     5701 gccaattaaa gagctgctca aacgcaccga agaacatatt aaaggcatgg tagtgcctgg
     5761 cacgttgttt gaggagctgg gctttaacta catcggcccg gtggacggtc atgatgtgct
     5821 ggggcttatc accacgctaa agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
     5881 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca ctttccatgc
     5941 cgtgctaaat ttgatccctc cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc
     6001 tattcaaaaa tctttggcga ctggttgtgc gaaacggcag cgaaagacaa caagctgatg
     6061 gcgattactc cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg taaattcccg
     6121 gatcgctact tcgacgtggc aattgccgag caacatgcgg tgacctttgc tgcgggtctg
     6181 gcgattggtg ggtacaaacc cattgtcgcg atttactcca ctttcctgca acgcgcctat
     6241 gatcaggtgc tgcatgacgt ggcgattcaa aagcttccgg tcctgttcgc catcgaccgc
     6301 gcgggcattg ttggtgctga cggtcaaacc catcagggtg cttttgatct ctcttacctg
     6361 cgctgcatac cggaaatggt cattatgacc ccgagcgatg aaaacgaatg tcgccagatg
     6421 ctctataccg gctatcacta taacgatggc ccgtcagcgg tgcgctaccc gcgtggcaac
     6481 gcggtcggcg tggaactgac gccgctggaa aaactaccaa ttggcaaagg cattgtgaag
     6541 cgtcgtggcg agaaactggc gatccttaac tttggtacgc tgatgccaga agcggcgaaa
     6601 gtcgccgaat cgctgaacgc cacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa
     6661 gcgttaattc tggaaatggc cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc
     6721 attatgggcg gcgcaggcag cggcgtgaac gaagtgctga tggcccatcg taaaccagta
     6781 cccgtgctga acattggcct gccggacttc tttattccgc aaggaactca ggaagaaatg
     6841 cgcgccgaac tcggcctcga tgccgctggt atggaagcca aaatcaaggc ctggctggca
     6901 taagctttgt gtgaggagat gtcgaatgag cgcacatgcc ctgcccaagg cagacctgac
     6961 cgccaccagc ctgatcgtat cgggcggcat catcgccgct tggctggccc tgcatgtgca
     7021 tgcgctgtgg tttctggacg cagcggcgca tcccatcctg gcgatcgcaa atttcctggg
     7081 gctgacctgg ctgtcggtcg gattgttcat catcgcgcat gacgcgatgc acgggtcggt
     7141 ggtgccgggg cgtccgcgcg ccaatgcggc gatgggccag cttgtcctgt ggctgtatgc
     7201 cggattttcg tggcgcaaga tgatcgtcaa gcacatggcc catcaccgcc atgccggaac
     7261 cgacgacgac cccgatttcg accatggcgg cccggtccgc tggtacgccc gcttcatcgg
     7321 cacctatttc ggctggcgcg aggggctgct tctgcccgtc atcgtgacgg tctatgcgct
     7381 gatccttggg gatcgctgga tgtacgtggt attctggccg ctgccgtcga tcctggcgtc
     7441 gatccagctg ttcgtgttcg gcacctggct gccgcaccgc cccggccacg acgcgttccc
     7501 ggaccgccac aatgcgcggt cgtcgcggat cagcgacccc gtgtcgctgc tgacctgttt
     7561 tcactttggc ggttatcatc acgaacacca cctgcacccg acggtgccgt ggtggcgcct
     7621 gcccagcacc cgcaccaagg gcgacaccgc ataagcttga gctgaggaga ttttgaatgc
     7681 aaacggaaca cgtcatttta ttgaatgcac agggagttcc cacgggtacg ctggaaaagt
     7741 atgccgcaca cacggcagac acccgcttac atctcgcgtt ctccagttgg ctgtttaatg
     7801 ccaaaggaca attattagtt acccgccgcg cactgagcaa aaaagcatgg cctggcgtgt
     7861 ggactaactc ggtttgtggg cacccacaac tgggagaaag caacgaggac gcagtgatcc
     7921 gccgttgccg ttatgagctt ggcgtggaaa ttacgcctcc tgaatctatc tatcctgact
     7981 ttcgctaccg cgccaccgat ccgagtggca ttgtggaaaa tgaagtgtgt ccggtatttg
     8041 ccgcacgcac cactagtgcg ttacagatca atgatgatga agtgatggat tatcaatggt
     8101 gtgatttagc agatgtatta cacggtattg atgcaacgcc gtgggcgttc agtccgtgga
     8161 tggtgatgca ggcgacaaat cgcgaagcca gaaaacgatt atctgcattt acccagctta
     8221 aataagcttt gcctcaggat ttttgaaatg acggtctgcg caaaaaaaca cgttcatctc
     8281 actcgcgatg ctgcggagca gttactggct gatattgatc gacgccttga tcagttattg
     8341 cccgtggagg gagaacggga tgttgtgggt gccgcgatgc gtgaaggtgc gctggcaccg
     8401 ggaaaacgta ttcgccccat gttgctgttg ctgaccgccc gcgatctggg ttgcgctgtc
     8461 agccatgacg gattactgga tttggcctgt gcggtggaaa tggtccacgc ggcttcgctg
     8521 atccttgacg atatgccctg catggacgat gcgaagctgc ggcgcggacg ccctaccatt
     8581 cattctcatt acggagagca tgtggcaata ctggcggcgg ttgccttgct gagtaaagcc
     8641 tttggcgtaa ttgccgatgc agatggcctc acgccgctgg caaaaaatcg ggcggtttct
     8701 gaactgtcaa acgccatcgg catgcaagga ttggttcagg gtcagttcaa ggatctgtct
     8761 gaaggggata agccgcgcag cgctgaagct attttgatga cgaatcactt taaaaccagc
     8821 acgctgtttt gtgcctcgat gcagatggcc tcgattgttg cgaatgcctc cagcgaagcg
     8881 cgtgattgcc tgcatcgttt ttcacttgat cttggtcagg catttcaact gctggacgat
     8941 ttgaccgatg gcatgaccga caccggtaag gatagcaatc aggacgccgg taaatcgacg
     9001 ctggtcaatc tgttaggccc gagggcggtt gaagaacgtc tgagacaaca tcttcagctt
     9061 gccagtgagc atctctctgc ggcctgccaa cacgggcacg ccactcaaca ttttattcag
     9121 gcctggtttg acaaaaaact cgctgccgtc agttaagctt gcaagactag cataacccct
     9181 tggggcctct aaacgggtct tgaggggttt tttggtgacg gtctcatgtt cgagaccaag
     9241 tctccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     9301 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9481 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10081 caagaagatc ctttga
//
