LOCUS       sequence-395621-        5598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains FLS2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP12
FEATURES             Location/Qualifiers
     source          1..5598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5184..5203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5185..5203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5213..5235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5429..5448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5548..5570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttttttgtta gagagatttt tgttgttttt
     2281 gttgttatgg ttagtacttt tactccgttt tatagatgga tcgggttaaa tttgttcaat
     2341 acataatgaa atgtctatct atgttggtaa tttctttcca ctaaaatatt tgaatttctt
     2401 tttacatgta tttattttca actattacca ttaatttctt tattagaaaa gtcatattca
     2461 acatgtttta tcaaaacttt ctgtcaaaag atgatattag aactaaattc ggacaatatt
     2521 ttagataatt cctcgtttgc gacgtaatac atgatgtcaa aactatgtta catttagtag
     2581 tacttatctt gaattaaata aaattaagaa aaaaatatga atggggtgtt actatggcat
     2641 atatctatta cctctggatt tcattctata tacaaaaata ttattttaaa atgataattt
     2701 acacgcgcca aatatgcgta ccagccacga ggcaaaaatt actatataac tttttttgaa
     2761 aataaaaact aattttttgt tttttcaaaa taagatacaa attatacgta attccttagt
     2821 aaagcctgaa aaacatcaaa ctttattttg gctaaaggac atcataaaac ctactatgta
     2881 attgtcatta acgtttgttt aactaaccaa aataaattac tgaatcttga actataaatt
     2941 cgaatataga gaaaatatga aattttgtaa gtgttattag acatccaaat tttaaaaccg
     3001 cgtaaactac actacaattt aaaatcggag tatgtaacct acatagagcc taaccacctc
     3061 ctaagccgta tttaataaca ttgttatatt atgtaaagat atgatatata tcgttaagta
     3121 atattatgta gtgaaaatcc gtcacaaatc ataataactt gtagtgatca aacttcttgt
     3181 atataatcta ctttgttctc gcgagcacct taatcaatca tcccatttaa aaaatttctt
     3241 tttgctatta acatcttaaa tgttaaatct atcattgagc aatgtaacag ttctgtgtga
     3301 agaaacaagg aatcacttac ttctacattg tctctttaca aaggaaattt ggcacatgat
     3361 aatgcttcgg tttgggcaat ctccttatat ctttgcagac ttgtctatta ccataggtaa
     3421 tcatataaaa gttattatat atgaacaatg ttcctatggt aatagaaatt cacattttat
     3481 taaaaaaact gatacgaata attcaaagaa ataaaataac ttttatatga ttgtccataa
     3541 gatatagtca aaacgcaaaa gactttgctc taagaaatat taaaaagaaa aacaacaaga
     3601 cgaggattat ttcttttcaa taattaaaac attagtataa atacaatcac atgcttaatg
     3661 taataatgtg acagtttggc cgagtggtct aaggcgccag atttaggctc tggtccgaaa
     3721 gggcgtgggt tcaaatccca cagctgtcaa tttgtttttt tctttgactc ctttgttcat
     3781 ctttatttta aaatcaatat aaaccctttt cggacattct aaatattttg acccaaccta
     3841 atgatattca ttatttgtct aacgggtcaa ctttcaagtc ttcaagtaaa catgatatgg
     3901 aattatatgg gtcaactcca agtaagaaca gtgcatgcat cttgaagaca aaattgattt
     3961 ttttttttgt ttttaattaa tggaaatttt gaaatactaa tgggtcaaga tgaggttgtc
     4021 caaatcaact gatatcatat atgaccagat tcctcaatag tcattagtca atacaaaatc
     4081 cataatcttt tttaaaaata taatcttttt attttaaatc gcctattttt ttccattcat
     4141 ttgtccaatg tgaaaagcca taaatcatat attaagaagt tgtgaattgt gatcatagga
     4201 gtaatatttc tatctgaaca aaaattgaat tttgttttgc tttttgttga aagataaata
     4261 aaatgtttgg cagattgttt gtacagaaca caacttgttg ataaaacaaa ttccatcaag
     4321 acagctaatg agcttttgat agtcagagct ttgaagacgt gttaatgaat taaactgatt
     4381 agacctaaaa ccaaatattt agaaagaaaa aaatatgata attgttatgt tatttaatca
     4441 atagtcaaat actatgttga gtaccaatca tttcagtttc aacgattgct actcttttga
     4501 ccaataacaa aaaggttttt atttttcatt tctcaaactc catttttgtt tgtttttagc
     4561 ataaaaacag ctagaatgtg agtttcatat gtattatata ctataagaaa tcatataaat
     4621 taacgactag aacatattgt tttcattcta gtggtatatc tgagtttgac tagaacgttt
     4681 ggaggcagat taaagtgatt tcgccttgtc aaaactactt tgtatatgtt atcaatcaat
     4741 cgctcaaaac taaatcggaa aaaaactaca taaataagat atggaaaatg atactaacac
     4801 aaactagtaa gtaagtagtc actacatatt ctggtcccat gaatatggct tacatagtac
     4861 atacattgtg agattaaatg aatatgatat ataatacaag catataagca tgtgcatgac
     4921 tatgaagtgg gaatacattt agtgccttta tatgcaactt gacagaatta ccaattatgt
     4981 gaatagtgaa acttatattg cgtcttggcc ttttcacatc cgtgacatga tccctcaaat
     5041 ctatatcttc ttgcggcata tgcttatata taaacatatg acttcgtcta tagatttata
     5101 tccagataat taattctctc ataatatctt ctccacaatt tcaactcttc taaagtctaa
     5161 accaacatga gaccgaattc tcgccctata gtgagtcgta ttacctggcc gtcgttttac
     5221 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5281 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5341 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5401 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5461 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5521 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5581 catcaccgaa acgcgcga
//
