LOCUS       sequence-395645-        5821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains RCD1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP35
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5407..5426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5408..5426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5436..5458)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5652..5671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5771..5793
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tggaggagca gattggacac cgttaaagac
     2281 gagatcttgg tggtatgtgc cttgcaaagt ctgatgtcgg gcacacgtcg atctcttttg
     2341 gtggtgaatt caatctggtt ggtgaaatag gaaattgaat ttgttcccaa aaataaacgg
     2401 atcccgaata tccgcggata tccagttttt tcacggatat tcgaaatccg gatatccgag
     2461 gtttacggat ccggatgcgg atcctaaaac tacatatccg ccggatacgg atacggatcc
     2521 ggatactcca aaaaacccgg atatccggat ccgtaacagg cctaatcgta ggttggtaaa
     2581 ttctagaaaa ataagagata taaaaactat atagttgtga catattcatt attcaatatg
     2641 agccatggct acacaaacta tatagttgtg acattttcat tattcaatat gagccatggc
     2701 tacacaaact atatagctgt gacatatcat acacagaata atattcccat gaatgcatgt
     2761 atatatagat cctttggttt tccaccgaat agtacaaacg tgtatatgat aaaagtttag
     2821 tcttgaattt tatagatctt acaagattgg gaagacagcc gttttcggta ccaactaaac
     2881 ccgtagtcac ggccggtcca tcttaatatg ctgtctaaag cccaaaaaat aaaaactggc
     2941 ccattataaa acaaagttga aaaaaattgc gaggtacaat ttatcaacta agtattcttc
     3001 atccgtctag acatcaaaag agttctaact ctctccaaga gatatcgatc tagtgttctt
     3061 tcttaagcca atcgaaatgt tatttatcca tcctcaaatt agccaattcc aaatattgtt
     3121 ttcaaattat agccttttcc aaagattgtg ttttcaatga ataattgtag aaatttaatt
     3181 ttgatagttt ttttataaga aaactacgca aaaagtccag acttaatatc caaggtgata
     3241 tcttttccaa gtcaatagtt cattgactat tatgcgtatc ttctatgttt taaaataaaa
     3301 taaattttgc tagtttagaa atgtgataat tattggaagt atatcaatgg aattttttat
     3361 atctcattta gtatgtctaa ttttgaaagt tatctgtatt gaaaatgatt aaatattctt
     3421 acccttataa accatatatc acttcaaaat ttatgctatt gtagtattgt ttatagaaaa
     3481 acggaaaaat aaaaatcatt acaaataaac gcggcaatga aacagaaaaa ataaaaacca
     3541 ttaaaaataa tcgcggcaaa tttaaataac ggctatcgaa ataatgaaaa ttattttttg
     3601 ttaacaaaca tttttctgga gaaagttttt aaaaattgat taaatattct tataactttg
     3661 taaaataata ttttcaaaaa cttctaaact atcctcaact aataaaattt tatttgccac
     3721 ttgcaataca actaaaactt tgttagacct tgtacgcaat aaagaaaata tttttcaatg
     3781 tcgatagcta tctccattcc aatggccccc ctagtctatt catcgattca caatcttatc
     3841 aatcatgttc cgtcaataca cacttttcta atttcaacaa ttttataatt gttgccctcg
     3901 ttacatttaa ttgtaatatt tttgttctcc tcatgataat attattccat tattgtattc
     3961 gaccatatct atctgcgtgt attgaccgcc tcttcaatta agtcaccacc caataagatt
     4021 tcgacgttga ctgaccgact tgtagcttta ataaagccca catgatttgc ttttcgtcca
     4081 agcaaatttt tggttagaga aagaggaaaa aaactcagga accgccgact taagaatttc
     4141 ttcttcatca tctctccaat atcgccgatt atatataaat gactcttcga ttctgtttct
     4201 tcttcgtcgt catcgccggc ttagggtttt ctctataagc aaatcttatc ttctcctttt
     4261 cctccgtttt ttctggtcag cttttgcttt tagattaccc ttgttgcctg cgatcgatct
     4321 gaaaaaagag gtccgtcatg ttcatgttca tgttcagttt cttttcgttt tgtttcaatt
     4381 cttctcatgc gatttgagga tttttttttt tatcttgaat tgtttttttt tgttgaagtc
     4441 gatgattgta gatcggctta gattcgtttg attttgagtg gttttgtgtt gttttgattg
     4501 agtttttgga tgattcgtgt gattatgctt acgatctgtg ttttttcgtc tctgatttta
     4561 gtttattttg aaaatcagtg aaactgcgat tgatttggtt cgatttgtgg attttgtgaa
     4621 gcaacagagc tattttgact tgagagatga attttaattt ttattgaaag ctaaaaagtt
     4681 gctggtttgg atgttgcagg aactttatct tgctgacggc tgttataaaa agacgtggca
     4741 tttcttctct gattgtgttt atttttatga cggagaagat gaacacactt gtaatctatc
     4801 agactctaac tgttactgtt gggtgagttt caagctcatg gcttgggttt attgttctca
     4861 tttttctgtg ttggtgattg ggtttacttg tttttgttgg tttgtttctg tgtttatctt
     4921 tagtgtctca taggatcgtt cttgttgagt gtgatgttca tttcttattg cttcattcct
     4981 atgtaggttg atttgtacct tccgtctttg taatggatct ttgtgctctt tataacacaa
     5041 gatacaacaa gaagtaatga aagactgttc ataatgttac ttactgatgg aaattgtagc
     5101 aaatgtcttt tgtgaaggaa tgatattaca ggatcatacg tctcatatag ttatgctgat
     5161 tctttcttgt ttactgattc tttatcatgt aaactgtgag gagtttttgt ttgactctat
     5221 tctctgtaat atagttgttc aatattttaa gccttttcca tttcttttgt gcagtgtttt
     5281 ctaatgctgt cggcaagaca ctatctttag gactgctagg gagtttagag gttacacagt
     5341 tgactttttc tatcaaggtt ataggtttag tattgttaat atatagaaca tgagaccgaa
     5401 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     5461 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5521 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5581 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     5641 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5701 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5761 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5821 a
//
