LOCUS       sequence-395648-        4779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains AOS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP51
FEATURES             Location/Qualifiers
     source          1..4779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4365..4384)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4366..4384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4394..4416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4610..4629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4729..4751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tacacttaga caccccaata ttttagattt
     2281 tactttaaag aaattcaact tttgtgtgtc ggggctctgt tcccaacata atgttttaag
     2341 cttcatttct tctatgactc atttgctgtt gatcaaacag ttaagaaaac tggtaagctt
     2401 taccaaaaaa gtacaatggt gatatatatt tggttagttt gaccagtgag aatgcatttt
     2461 atcacattta tgtaaaccac tcaacaaaga acctctagca agcgtcggac aagaagttta
     2521 agattactaa ggccagaatt aattactacc ttgttcacat gttgtatgat catcgtagca
     2581 taatgtctta gaacaaacag atcagctaca tgttaagctc tacgaccaaa atgttgaagg
     2641 gctcgaggct aatgtagttt ggtgtctctt atgaaaaagc tactccacag aggcttcaaa
     2701 tattatcttt taagcacaag acacatgcga ttaaagtagc attgaggact tcgtgattgt
     2761 gattacttcc atgtaacgcc ctcgatgcaa attaaaaatg ttgttttcat caatagccac
     2821 tcctaactcg gaagtgttta gttatgacaa tcaaaaactt aagagaattt tctgtgtatt
     2881 ccaatatgtt atgaggttta ttcaaagaga aggacaccta agtattttct ttccaacaag
     2941 gcaaaaaaca ggtaaacgct tgagacaaat attatcatgt ttgtccaaaa atagtgagta
     3001 tcatgtgaat aatttagaag ctagatatgt tcgtcaataa acaataaaat gcgctaggat
     3061 acaaaacgaa cgcttgggac gtgttttagg tacaaaactt caaaaaaatc tttatcgaga
     3121 cagatttcca gataaaatac acataccatt ttaacaacgt gtgtgtttat acaaaaataa
     3181 caaaatatta attttacgat ttgaagatat atattgtcag aaggggaaaa gaaaaaaaca
     3241 aaacaaaaag atacaaaaca gagtaacaaa ctctataaaa tatgataata taatacataa
     3301 ttgggaaaat gactaaacat tatctctgcc ttttggaact catcaataat tatatatttt
     3361 tttatttctc ataaaagtca aaactatcaa ttcttaaatt cattctaatt cactcaactt
     3421 ttttttataa aaaaatatct atattaagtt ctcgatatta tttacttcca ttaatttatc
     3481 aaatgatttt ggtatagaat caacttataa tcatggatca agtcgtggaa taacatatgc
     3541 tcaagggatg gagctaaaag tgcagtctac caaacctcaa gtgtttgtaa ctttgtataa
     3601 gctaggcctc cgattattaa ctgaatcggt ttttctggat caaaccaaac tgagatttgg
     3661 ggtttggtta accgtccggt ttataagagt cagccagtca gttagatttt gaaataaggt
     3721 cggataacag tttggtttgg tttatcgaac caaacggtta accatttgtt taaaattttc
     3781 caatttaata attttacgga tattttacgt aatgatatca aaaattcttc aaaacactac
     3841 gtgaaccaaa aaaaaactat tcaaatattt ttaaagtctc aaatgcttgt ataaactatt
     3901 gatagtacat tttcaggata tattataaga agaggaatat taaacaaaga aacaaaaata
     3961 caaaacagag caacaagctc gataaaatat gagaataaat agtaccaact tttacacaac
     4021 aaaaacatta ccaatttttt aatatgtcag aaaaaataaa aaaagtacca actttataaa
     4081 atgaaggaaa aaagagtcaa agcacgtggc taaatgaatc ggccggtggc cagagtctcc
     4141 aatagatctc tttaataact gcgtggtctg aaaaaggaat cttccttcca cggccactaa
     4201 attcactatt ttcattcaca tttattattt tcctttataa atacaaattc atttctacac
     4261 aataatcatt caatacacat aatttacttc tttctttata actaccatat tctcaatcac
     4321 aacactcgcc actgtttcga atagaacatg agaccgaatt ctcgccctat agtgagtcgt
     4381 attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4441 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4501 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     4561 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4621 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4681 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4741 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
