LOCUS       sequence-39565-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1392
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(144..155)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggcg cgccgcgaga
       61 ggaactttac cggtcctggg ggcacgccga ggctctgctt ggataaaggg aaaagaggtg
      121 gttacccggc cgttcctccc tacttgcaac tcccaagacc acccgggagc gccttagaca
      181 ctgggatgag cgcgcgctga gcggacgcag ggttcccaga aaagggcgcg cgtccgctgg
      241 aaaaatttgg aaacagaggt tggggcacgc cggaactcaa gagttggaga cttctgacag
      301 ccctctgggg gcggctgaag accctgcagc acttttcctc gttcacccac tctcgcctgc
      361 cccaaatggc tgggattgaa ctcaccgagg ctgagggcag cgtgccgacc ccctccccag
      421 ctcaggtccg cgacacttca acagccacag gagccttctc ggacacttaa cgtcactgca
      481 ctacaaactc gctagccctc tgctggcgcg tctcggcccg ggcctcccag gcagacctag
      541 ccctgtcccg cgctcagggc gcactcacgt gtccggcggc gag
//
