LOCUS       sequence-395666-        5354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains BIK1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP22
FEATURES             Location/Qualifiers
     source          1..5354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4940..4959)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4941..4959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4969..4991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5185..5204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5304..5326
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcgttcccaa atctcggtca attgttcaaa
     2281 tttcaaaccc agaacttatg aataaaattg aattgaattg aatttgagtc tgtacaaaat
     2341 aaaaaacgcg tatcgtcccc tttcacgagt cttgtgtgtg attgatttta ttatattata
     2401 tagcgatgag agagacagag cttgaaggtt ctttttagcg aaagagaaaa atccaggaag
     2461 ataggcgaaa aggaagatga agcgaagatg aggttaatat aatactcatg ttaaatgaca
     2521 aaaatgccct tatatgatta atgatattac catttgagct tgctgtggaa gctgtaacga
     2581 accgaaaatt aaaaacagaa taacgaacat agacggagaa tatgatatta ttcgttttac
     2641 caaagaaact aacaaatagt tttaacttta tctaacaaag gggtaaaacg ggtaatttgt
     2701 ttgggatgag gtggagcgta gcggacaatc gagaaattaa aagtttggct tggggacgaa
     2761 gttaaaggtg ggctttaacg ttttaaattg gctgactcgg acgatatttc ttgtatttaa
     2821 taccaaaaat gaatgacttt ataattcatt tgtagattga aagttacgta ttgattcgaa
     2881 aatcaacaca ttgtgttttc aagtgggcat aaactataac accttgttga ttgattaata
     2941 gattacctaa agacattatg gtttattact ggtctttcaa tatattttta tcgcattgtc
     3001 aatgatattg tttttgtatc ccaagtccac tgttttggtc tgtacattca ttttgattgg
     3061 gatttatctt tttaaaattt cttctaatgt tttttcgata tggttattac ttgctttgat
     3121 tttcttttca gtatgtgtat tgctttgcaa attgtttttt tcttaagatg aaaaacaact
     3181 cattaaattg tttgagaaat actactaaaa caaataaaca atgaggagaa ttatggaaaa
     3241 caaagtgtaa taggctttaa ttcattgcta gtgggctttt tgggcctatg ggcatattac
     3301 ttaccactat ccaacccaaa atgccaaata accgacatgt ctcacacaat ccaattttgg
     3361 gccatacggc cgaaattatt taaacctgtg ctcataattt actttacaaa ttattacttt
     3421 tccataaatt gtggaaaagt tatctgtaac atccgattca actggagtct agactactat
     3481 agacattgat acgttttgag tttttagata cttggaagat atatgcattt atgaatacag
     3541 attacagaca catactagta ctactgtatg tctgtatatg gatacaaaaa aaatcatgta
     3601 tgaatactaa aattttatta gaaatctatt tttcaattgt tgcaacaatc aagttgtcaa
     3661 atttattttt gtaaccgtta aacaaacaaa tatcgattta ggtttctaat ctgaattgac
     3721 atctcaaaca aaaaaggctg aatactttct gaaaatagtg tatggaatga aggtggcttt
     3781 tagagccatt ataaccggaa gaaaattcag gtgactttta gaaccattat aaccggaaga
     3841 aaaggtgaat tttaattttt agctgtgtgg aagacacggc aagtccaagt agtaccttcg
     3901 tacgtcaata ttgtccaacc ggccgtgtcg aaaatcttct tgagaaaaat tggattttca
     3961 tctataaaaa aaaaaagtcc aagtaatacc aaacaaagac agcgacgtgt aaaacaatac
     4021 aagactcata atcacaaacc taccacccaa gtcaaaccta tattccattt agtgaattct
     4081 tgattatgac ttcttgaaat catttgtatt catatgtata attatttaag tcatttttct
     4141 gtaagtaaaa tttttatata tctagaataa cgagttccct acgacaagat acagttgaac
     4201 gtaaatgtga catctcaatt ttcattggtg tctagtactc tagtgattag gttttcgaca
     4261 tttattgtac tgattaagta aaaattcatg gtacaaacat cgaatatata tttttctgct
     4321 tacacacacc aattaacgtg gatagaccaa ttgaaatatt ttgttacgac aaagcaaaac
     4381 aaaacaaacg tcatgtttcg ctgtttgttt gtcgtcccgt taatggtaat ctttcagaca
     4441 catacagtac ccaaacaagt aatttgacta aaattttctc tctgtctaaa tttcagaaga
     4501 aaaaaaaact ttaggatata ttgccaaaag atcttaaaaa tgggtcatat cattttgatc
     4561 atatagaatc caacgacctt tatcttttcg ccgaactata cttttttgtg tccatttgtt
     4621 tgactttctt tcacacacac atccacaaag aaaaaaggac cattcttctc cttcttctag
     4681 tcacccctcg tgcctctctt taacaccaaa cccaaaactc ccttctcttt cttccttcct
     4741 ctccgatctc cgttcacatc tctctctcat ctttatcttc ttcttttttt gccttgtggg
     4801 ttgaaagttt ctatattttc tctttctctt ctgtttacat aatccatttt cagctcaagc
     4861 agctgaagaa taacgatcaa gaaccaaaaa agaagaaaac gaatctgttc ttagctttga
     4921 acatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg
     4981 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5041 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5101 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5161 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5221 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5281 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5341 accgaaacgc gcga
//
