LOCUS       sequence-395672-       12502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2363
FEATURES             Location/Qualifiers
     source          1..12502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8359..8947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8412..8432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8830..8841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9019..9252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9330..9451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9367..9386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9421..9440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9491..9626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9646..9665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9647..9665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9675..9692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9675..9691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9684..9706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9833..10288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9920..9939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10130..10151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10314..10418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10419..10487,10488..11279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10637..10656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11450..12038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11939..11958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12192..12209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12326..12347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12362..12367,12368..12385,12386..12392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12400..12416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12405..12427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12424..12440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12424..12440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12459..12479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12461..12479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagc ctggagcgcg agcttcgcca
     4141 gctgagcaag gcgaaagcca aggcgcagag ggccgggcag cggcgcgaag aggccgcgct
     4201 gtgccaccag ctgggggagc tcctggccgg ccatggccgc tacgccgagg ctctggagca
     4261 gcactggcag gagctgcagc ttcgggagcg cgctgacgac cctctgggct gtgccgtggc
     4321 ccaccgcaag atcggagagc gcctggccga gatggaggac tacccggctg ccttgcagca
     4381 ccagcaccag tacctggagc tggcacattc cctgcgcaac cacacggagc tgcagagggc
     4441 ctgggccacc atcggccgca cccacctgga catctatgac cactgccagt cgagggatgc
     4501 tttgctgcag gcacaggctg cctttgagaa gagcttggct attgtggatg aggagctgga
     4561 ggggacactg gcccagggag agctgaatga gatgaggacc cgcctctatc tcaacctggg
     4621 cctcaccttt gagagcctgc agcagacagc cctgtgcaac gattacttca ggaagagcat
     4681 cttccttgcg gagcagaacc acctttacga ggacctattc cgcgcccgct acaacctggg
     4741 caccatccac tggcgcgcgg gccagcactc ccaggctatg cgctgcttgg agggtgcccg
     4801 ggagtgtgcg cacaccatga ggaagcggtt catggagagc gagtgctgcg tggttattgc
     4861 acaggtcctc caagacctgg gagacttttt ggctgccaag cgagccctga agaaggccta
     4921 caggctgggc tcccagaagc ctgtgcagag ggcagccatc tgtcagaacc tccagcatgt
     4981 gctggcagtg gtccggctgc agcaacagct ggaagaggct gagggcagag accctcaggg
     5041 tgccatggtc atctgtgagc agctagggga cctcttctcc aaggcaggag actttcccag
     5101 ggcagctgag gcttaccaga agcagctgcg ttttgctgag ctgctggaca gaccgggtgc
     5161 tgagcgggcc atcatccacg tgtccctggc caccacactg ggagacatga aggaccacca
     5221 tggggccgtg cgccactatg aggaggaact gaggctgcgc agcggcaacg tgctggagga
     5281 ggccaagacc tggctgaaca ttgcactgtc ccgcgaggag gccggcgatg cctacgagct
     5341 gctggccccg tgcttccaga aagcgctcag ctgtgcccag caggcccagc gtccccagct
     5401 gcagaggcag gtcttgcagc atctccatac cgtgcagctg aggctgcagc cccaggaggc
     5461 ccctgagacc gaaaccagac tacgggagct cagtgtagct gaagatgaag atgaggagga
     5521 ggaggcggag gaggcggcag ccacagcgga gagcgaagcc ctggaggccg gcgaggtgga
     5581 gctctcagag ggcgaggacg acaccgatgg cctgaccccg cagctggagg aggacgagga
     5641 gcttcagggc cacctgggcc ggcggaaggg gagcaagtgg aaccggcgaa acgacatggg
     5701 ggagaccctg ctgcaccgag cctgcatcga gggccagctg cgccgcgtcc aggaccttgt
     5761 gaggcagggc caccccctta accctcggga ctactgtggc tggacacctc tgcacgaggc
     5821 ctgcaactac gggcatctag aaattgtccg cttcctgctg gaccacgggg ccgcagtgga
     5881 cgacccaggt ggccagggct gcgaaggcat cacccccctc cacgatgccc tcaactgtgg
     5941 ccacttcgag gtggctgagc tgctgcttga acggggggcg tccgtcaccc tccgcactcg
     6001 aaagggcctc agcccgctgg agacgctgca gcagtgggtg aagctgtacc gcagggacct
     6061 ggacctggag acgcggcaga aggccagggc catggagatg ctgctccagg cggctgcctc
     6121 gggccaagat ccccacagct cccaggcctt ccacacccca agcagccttc tgtttgaccc
     6181 cgagacctct cctcctttga gcccctgccc agaacccccc tctaatagca ctagactccc
     6241 agaggcctct caggcccatg tcagggtctc cccagggcag gcggcaccag ccatggccag
     6301 gcctcggagg agcaggcatg ggccagccag cagcagcagc agctcagaag gcgaggacag
     6361 cgcaggcccc gcacggccgt cccagaagag gcctcggtgc tcggccacag cacaacgggt
     6421 ggcagcctgg acgcctggcc ccgccagcaa cagggaagca gccacagcca gcaccagccg
     6481 ggcagcctac caggcagcca tccggggtgt gggcagtgct cagagccggc tggggcctgg
     6541 cccaccgcgg ggccacagca aagcccttgc cccccaggca gcgctcatcc cggaggagga
     6601 gtgcctggcc ggggactggc tggagctgga catgcccctg acccgcagcc gccggccccg
     6661 cccccggggc actggagaca accgcaggcc cagtagtacc tctgggtcgg acagtgagga
     6721 gagcaggccc cgtgcccgag ccaagcaggt ccgcctgacc tgcatgcaga gttgcagtgc
     6781 gccagttaac gcagggccca gcagcctggc ttcagaacct ccagggagcc ccagcacccc
     6841 cagggtctca gagcccagtg gggacagctc tgcggcaggc cagcccttgg gtccggcccc
     6901 gccccctccc atccgggttc gagttcaagt tcaggatcat ctcttcctca tccctgtccc
     6961 acacagcagt gacacccact ctgtggcctg gctggccgag caggcggccc agcgctacta
     7021 ccagacctgc gggctgctgc ccaggctcac cctacggaaa gagggggccc tgctggcccc
     7081 acaggacctc atccctgatg tgctgcagag caatgacgag gtgttggctg aggtgacttc
     7141 gtgggacctg cccccgttga ctgaccgcta ccgcagggcc tgccagagcc tggggcaagg
     7201 ggagcaccaa caggtgctgc aggccgtgga gctccagggc ttgggcctct cgttcagcgc
     7261 ctgctccctg gccctggacc aggcccagct tacacccctg ctgcgggccc tcaagctgca
     7321 cacagcactc cgggagctgc gcctggcagg gaaccggctg ggggacaagt gtgtggctga
     7381 gctggtggct gccctgggca ccatgcccag cctggccctc cttgacctct cctccaatca
     7441 cctgggtccc gaaggcctgc gccagcttgc catggggctc ccaggccaag ccaccttgca
     7501 gagtttggag gagctggact taagcatgaa ccccctgggg gacggctgtg gccagtccct
     7561 ggcctccctc ctgcacgcct gccccttact cagcaccctg cgcctgcagg cgtgtggctt
     7621 cggccccagc ttctttctga gccaccagac agcactgggt agtgctttcc aagatgctga
     7681 gcacctgaag accctgtccc tgtcctacaa cgccctggga gcccctgccc tggccaggac
     7741 cctgcagagc ctgcccgccg gcaccctcct gcacttagag ctcagctccg tggcagccgg
     7801 caagggtgat tcggacctca tggagcctgt attccgatac ctggccaagg aaggctgtgc
     7861 tctagcccac ctgaccctgt ctgcaaacca cctgggggac aaggctgtta gagacctgtg
     7921 cagatgtctc tctctgtgcc cctcactcat ctcactggat ctgtctgcca accctgagat
     7981 cagctgtgcc agcttggaag agctcctgtc caccctccaa aagcggcccc aaggccttag
     8041 cttccttggc ctgtcaggct gcgccgtcca gggtcccctg ggcctgggcc tgtgggacaa
     8101 gatagccgcg cagctccggg aactgcagct gtgcagcaga cgcctctgcg ctgaggacag
     8161 ggacgccctg cgccagctgc agcccagtcg gccgggcccc ggcgagtgca cgctggacca
     8221 cggctccaag ctcttctttc ggcgcctcta gtaatgaact agtccgtaac ttgaaagtat
     8281 ttcgatttct tggctttata tatcttgtgg aaaggacgac aacgactgct ggttggctca
     8341 gacacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8761 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8941 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9241 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     9301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     9361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     9481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9601 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     9661 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10261 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
    10321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    11041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    11101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    11161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    11221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    12241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    12301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    12481 aacaaaagct ggagctgcaa gc
//
