LOCUS       sequence-395729-       12550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12550)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2718
FEATURES             Location/Qualifiers
     source          1..12550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8407..8995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8460..8480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8878..8889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9067..9300
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9378..9499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9415..9434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9469..9488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9539..9674
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9694..9713)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9695..9713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9723..9740)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9723..9739)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9732..9754)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9881..10336
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9968..9987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10178..10199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10362..10466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10467..10535,10536..11327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10685..10704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11498..12086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11987..12006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12240..12257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12374..12395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12410..12415,12416..12433,12434..12440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12448..12464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12453..12475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12472..12488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12472..12488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12507..12527
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12509..12527
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggca gccctgctga gatccgcgcg
     4141 ttggttgctg cgtgccgggg cggccccgcg cctcccgctc tccctgcgcc tcctccctgg
     4201 cggcccgggc cggctgcatg ccgcctccta tctgcccgcc gctcgcgccg ggcccgtggc
     4261 cggaggacta ctgagcccag ccaggctgta tgccattgct gccaaagaaa aagatattca
     4321 agaggagtcc actttttctt ctaggaagat ttccaatcag tttgattggg ctctaatgag
     4381 actagatctt tctgttcgaa gaactggccg cattccaaag aagcttctac aaaaagtttt
     4441 taatgatacc tgccgctcag gtggcctagg tggtagtcat gccttgcttc tactacgtag
     4501 ttgtggttct ctcttgcctg aactaaagct tgaagagaga acagaatttg ctcataggat
     4561 atgggacaca cttcagaaat taggtgctgt gtatgatgtg agtcactata atgctttact
     4621 taaagtctat cttcaaaatg aatataaatt ctcaccaact gatttcctgg caaaaatgga
     4681 ggaagcaaac attcaaccaa atcgagtgac ataccagaga ttgattgctt cttattgtaa
     4741 tgtaggagat attgaaggtg ccagcaagat tcttggattt atgaaaacta aggatctccc
     4801 agttacagag gcagtattca gtgcccttgt gacagggcat gccagagctg gtgatatgga
     4861 gaatgcagaa aacattctca cagtgatgag agatgccgga attgagcctg gtccagacac
     4921 atacctcgca ttattgaatg catatgctga gaagggcgac attgaccatg ttaagcagac
     4981 tctggagaag gtggagaagt ccgagcttca ccttatggac cgtgatttac tgcaaattat
     5041 ttttagcttc agtaaagctg ggtatcctca gtatgtctca gaaattttgg aaaaagttac
     5101 atgtgaaaga agatatattc cagatgcaat gaacctcatt ttacttttag tcactgaaaa
     5161 attggaagat gtagcgttgc aaattttact agcatgcccc gtatcaaagg aagatggccc
     5221 aagtgtcttt ggcagtttct ttttacaaca ctgtgtgact atgaatacgc ctgtggagaa
     5281 gctaacagac tactgtaaga agttaaagga agtccagatg cactcctttc ctctgcagtt
     5341 caccctccat tgtgctttac tcgccaataa aactgatttg gcaaaagcct taatgaaggc
     5401 tgtgaaggag gaaggttttc ctatcagacc tcactatttc tggccattgc tagttggacg
     5461 tcggaaggaa aaaaatgttc aaggtataat tgaaatcctc aaaggaatgc aagaattggg
     5521 agtacatcct gatcaggaaa catatacaga ttatgtgatt ccatgctttg atagtgtaaa
     5581 ctcagcacga gccattttgc aggaaaatgg atgtctgtct gatagtgata tgttttctca
     5641 agctggattg agaagtgaag cagcaaatgg gaacttagac tttgtattat catttttgaa
     5701 atcaaataca ttgcccatct cgctgcagtc tataagaagt agcctactgc taggcttcag
     5761 gaggtctatg aatataaatc tttggagcga gataacagaa ttgttgtaca aggatggacg
     5821 ttattgccag gagcctcgag gaccgacgga agctgttggc tattttcttt ataacttgat
     5881 tgacagcatg agtgactcag aggtacaggc caaggaggag catttgagac aatacttcca
     5941 tcagctggag aagatgaatg taaaaattcc tgaaaatatc tacagaggca ttcgtaatct
     6001 cctggaaagc taccatgttc ctgaattgat taaggatgct cacttgttgg ttgagagtaa
     6061 gaatttagac tttcaaaaaa ctgtgcaact tacatcatct gaattggagt ccacacttga
     6121 aacactaaaa gctgaaaatc aacctataag agatgtccta aagcaactca tattagtgct
     6181 ttgttcagaa gagaatatgc aaaaagccct tgaattgaaa gcaaaatatg aatccgacat
     6241 ggttactggt ggctatgcag ctttaataaa tttatgctgt cgacatgata aagtagaaga
     6301 tgccttgaac ttgaaagaag aatttgaccg cttagattca tctgctgtcc ttgacaccgg
     6361 caagtatgta ggccttgtaa gagtattggc aaagcatggc aagctccaag atgctattaa
     6421 cattctgaag gagatgaaag agaaggatgt tcttatcaaa gatacaacag ccttgtcctt
     6481 tttccacatg ctaaatggcg cagctttaag aggtgaaatt gaaacagtaa aacagttgca
     6541 tgaagccatc gtgactctag ggttagcaga accatccacc aacataagtt tcccattggt
     6601 cactgtacac ttggaaaagg gcgacctatc tactgctctt gaggtcgcca ttgactgcta
     6661 tgaaaagtat aaagtattac caaggattca tgatgtcttg tgtaaactgg tagagaaagg
     6721 cgagactgat ctaattcaga aagcaatgga ctttgtgagc caagaacaag gtgaaatggt
     6781 gatgctctat gatctcttct ttgccttcct acaaacagga aattacaaag aggccaagaa
     6841 gatcattgag actccaggga ttagagctcg atctgcaagg cttcagtggt tttgtgacag
     6901 atgtgttgca aataatcagg ttgaaactct ggaaaaatta gtggagctga cacagaagct
     6961 atttgaatgt gatagagacc agatgtacta caatctgcta aaactgtata aaataaacgg
     7021 tgactggcaa agagctgatg cagtctggaa taaaatccaa gaagaaaatg ttattcctcg
     7081 tgaaaagaca ttaagattat tagcagaaat ccttagagag ggtaaccagg aagttccgtt
     7141 tgacgtacct gagttgtggt atgaagatga aaaacattcc ctgaattctt cgtcagcctc
     7201 aaccacagaa cctgatttcc agaaagatat attgattgcc tgccgattga accaaaaaaa
     7261 aggggcatat gatattttcc tgaatgcaaa agagcaaaac attgtgttta atgctgaaac
     7321 ctacagcaat ctcattaaat tactgatgtc agaagattat tttacacaag caatggaagt
     7381 gaaagcattc gcggagaccc acatcaaggg cttcacactg aacgatgctg ccaacagccg
     7441 cctcatcata acgcaagtta ggcgggatta tttgaaagag gctgtgacaa cactgaaaac
     7501 agtattggat cagcagcaga ccccttctag gttagcagtg acccgtgtca tccaggcatt
     7561 ggccatgaag ggtgatgttg aaaacataga agtagttcag aagatgttaa atggactcga
     7621 agactccatt ggactttcaa aaatggtttt catcaataac attgctttgg ctcaaataaa
     7681 gaataataac atagatgccg caatagaaaa cattgaaaat atgcttactt cagagaataa
     7741 agtcattgaa ccccaatact tcggcttggc atacttattc agaaaagtaa tagaggagca
     7801 gttggaacca gcagttgaaa agataagcat catggcggag agattggcca atcagtttgc
     7861 aatttataaa cctgtcactg attttttcct tcaacttgtg gatgcaggca aggtggatga
     7921 tgccagagct ctcctacaga gatgtggtgc aattgctgaa caaaccccga ttttgttgtt
     7981 gttcctcctt aggaattcta ggaaacaagg aaaggcatca actgtgaaat ctgtgttaga
     8041 attgattcct gaattaaatg aaaaggaaga agcatacaat tccctcatga aaagctatgt
     8101 ctcagagaaa gatgtcacat ctgctaaagc actgtatgaa catttgactg caaagaatac
     8161 aaaattggat gatctgtttc taaagcgtta cgcatctttg ctgaagtatg ctggagagcc
     8221 tgtccctttc attgaacccc ctgaaagctt tgaattttat gcacagcagc taagaaaatt
     8281 gagggaaaac tcttcttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     8341 gctttatata tcttgtggaa aggacgatgg gaaggactat gccttaaccg gacgcgttaa
     8401 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8461 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8521 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8581 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8641 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8701 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8761 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8821 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8881 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8941 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     9001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9061 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     9121 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9181 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9241 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9301 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9361 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9421 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9481 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9601 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9661 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9721 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9781 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9841 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10321 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    11041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12301 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12361 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12421 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12481 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12541 agctgcaagc
//
