LOCUS       sequence-39581-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1409
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggaagt tcctatacct tttgaagaat aggaacttcg
       61 gcgcgcctgg agggtgaaac ccgctattga caagactggg tttggctcag gcaggagagg
      121 cgctttctat atctccttgg gaaaggccgg gtttgcgtca gtgcaaagca aggtgccctt
      181 ggtttgacat tttcttgata atacaagttt gataaatcat tacccccttg gattcaagtt
      241 ttaaagggac aacagcctag agtatgctgg ccagttgtca ctccagcggg agggggctgc
      301 gtgtttggcg tgactcatgt ggtctgatta ctaaaccgaa accggcagga ggggaagaca
      361 gaagtttgcc tcctcctaat taagggaaac atgggaggga cacgtcccac tgcacccaag
      421 gcaaggcgca gccgccaaca gagtggatcg ggctgtagag gaggaaggag ggggttaaat
      481 cactgtctat tctttccctt tcactagatt ccattgctgt ccccccttat cttcagggat
      541 tccgaaaata ttactccgaa agaggttgag ggttcattaa ttaactaggc cagagacatt
      601 aaaaaggaca cttagtttgg ttcacttgaa atta
//
