LOCUS       sequence-395810-       11329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1132
FEATURES             Location/Qualifiers
     source          1..11329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7186..7774
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7239..7259)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7657..7668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7846..8079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8157..8278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8194..8213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8248..8267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8318..8453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8473..8492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8474..8492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8502..8519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8502..8518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8511..8533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8660..9115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8747..8766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8957..8978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9141..9245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9246..9314,9315..10106)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9464..9483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10277..10865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10766..10785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11019..11036
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11153..11174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11189..11194,11195..11212,11213..11219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11227..11243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11232..11254
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11251..11267
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11251..11267
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11286..11306
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11288..11306
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcgg tgtagaaaca tgttcttctc
     4141 atttaaggca tctctttgtg gctgcggggc tgccaccgct ccgagtctga cagctatcag
     4201 ctgtactctg aactgtagtt gccaaagttt caaacccggg aaaataaacc accgtcagtg
     4261 tgaccaatgc aagcatggat gggtggccca cgctctaagt aagctaagga tcccccccat
     4321 gtatccaaca agccaggtgg agattgtcca gtccaatgta gtgtttgata ttagcagcct
     4381 catgctctat gggacccagg ccatccccgt tcgcctaaaa atcctactgg accggctctt
     4441 cagtgtgttg aagcaagatg aggttctcca gatcctccat gccttggact ggacacttca
     4501 ggattatatc cgtggatacg tactgcagga tgcatcagga aaggtgttgg atcactggag
     4561 catcatgacc agtgaggaag aagtggccac cttgcagcag ttccttcgtt ttggagagac
     4621 caaatctata gttgaactca tggcaattca agagaaagaa gagcaatcca tcatcatacc
     4681 accttccaca gcaaatgtag atatcagggc tttcatcgag agctgcagtc acaggagttc
     4741 tagcctcccc actcctgtgg acaaaggaaa ccccagcagt atacacccct ttgagaacct
     4801 cataagcaac atgactttca tgctgccttt ccagttcttc aaccctctgc ctcctgcact
     4861 gatagggtca ttgcccgaac aatatatgtt ggagcagggt catgaccaaa gtcaggaccc
     4921 caaacaggaa gtccatgggc ccttccctga cagcagcttc ttaacttcca gttccacacc
     4981 atttcaggtt gaaaaagatc agtgtttaaa ctgtccggat gctattacta aaaaagaaga
     5041 cagcacccat ttaagtgact ccagctcata caacattgtc actaagtttg aaaggacaca
     5101 gttatcccct gaggccaaag tgaagcctga gaggaatagc cttggtacaa agaagggccg
     5161 ggtgttctgc actgcatgtg agaagacctt ctatgacaaa ggcaccctca aaatccacta
     5221 caatgccgtc cacttgaaga tcaagcataa gtgcaccatc gaagggtgta acatggtgtt
     5281 cagctcccta aggagccgga atcgccatag cgccaacccc aaccctcggc tgcacatgcc
     5341 aatgaacaga aataaccggg acaaagacct caggaacagc ctgaacctgg ccagctctga
     5401 gaactacaag tgcccaggtt tcacagtgac gtccccagac tgtaggcctc ctcccagcta
     5461 ccctggttca ggagaggatt ccaaaggcca accagccttc ccaaacattg ggcaaaatgg
     5521 tgtgcttttt cccaacctaa agacagtcca gccagtcctt cctttctacc gcagtccagc
     5581 cacgcctgcc gaggtagcaa acacgcctgg gatactccct tccctcccgc tgttgtcctc
     5641 ttcaatccca gaacagctca tttcaaacga aatgccattt gatgcccttc ccaagaagaa
     5701 atccaggaag tccagtatgc ctatcaaaat agagaaagaa gctgtggaaa tagctaatga
     5761 gaaaagacac aacctcagct cagatgaaga catgccccta caggtggtca gtgaagatga
     5821 gcaggaggcc tgcagtcctc agtcacacag agtatctgag gagcagcatg tacagtcagg
     5881 aggcttaggg aagcctttcc ctgaagggga gaggccctgc catcgtgaat cagtaattga
     5941 gtccagtgga gccatcagcc aaacccctga gcaggccaca cacaattcag agagggagac
     6001 tgagcagaca ccagcattga tcatggtgcc aagggaggtc gaggatggtg gccatgaaca
     6061 ctacttcaca cctgggatgg aaccccaagt tcctttttct gactacatgg aactgcagca
     6121 gcgcctgctg gctgggggac tcttcagtgc tttgtccaac aggggaatgg cttttccttg
     6181 tcttgaagat tctaaagaac tggagcacgt gggtcagcat gcattagcaa ggcagataga
     6241 agaaaatcgc ttccagtgtg acatctgcaa gaagaccttt aaaaatgctt gtagtgtgaa
     6301 aattcatcac aagaatatgc atgtcaaaga aatgcacaca tgcacagtgg agggctgtaa
     6361 tgctaccttt ccctcccgca ggagcagaga cagacacagc tcaaacctaa acctccacca
     6421 aaaagcattg agccaggaag cattggagag tagtgaagat catttccgtg cagcttacct
     6481 tctgaaagat gtggctaagg aagcctatca ggatgtggct tttacacagc aagcctccca
     6541 gacatctgtc atcttcaaag gaacaagtcg aatgggcagt ctggtttacc caataacgca
     6601 agtccacagt gccagcctgg agagctacaa ctctggcccc ttgagcgagg gcaccatcct
     6661 ggatttgagc actacctcga gcatgaagtc agagagtagc agccattctt cctgggactc
     6721 tgacggggtg agtgaggaag gcactgtgct tatggaggac agtgatggga actgtgaagg
     6781 gtcgagcctt gtccctgggg aagatgagta ccccatctgt gtcctgatgg agaaggctga
     6841 ccagagcctt gctagcctgc cttctgggtt gcccataacc tgtcatctct gccaaaagac
     6901 atacagtaac aaagggacct ttagggccca ctacaaaact gtgcacctcc ggcagctcca
     6961 caaatgcaaa gtaccaggct gcaacaccat gttttcgtct gttcgcagtc gaaacagaca
     7021 cagccagaat cccaacctgc acaaaagcct ggcctcatct ccaagtcacc tccagtagta
     7081 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7141 ggacgacacg ttacgcttac gacggtgacc acgcgttaag tcgacaatca acctctggat
     7201 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7261 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7321 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7381 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7441 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7501 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7561 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7621 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7681 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7741 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     7801 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7861 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7921 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7981 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8041 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8101 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8161 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8221 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8281 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8401 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8461 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8521 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8581 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8641 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9061 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9241 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9301 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9361 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9421 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9481 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9541 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9601 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9661 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9721 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9781 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9841 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9901 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9961 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10021 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10081 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10141 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10201 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10261 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10381 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10441 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10501 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10561 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10621 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10681 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10741 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10801 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10861 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10921 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10981 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11041 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11101 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11161 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11221 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11281 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
