LOCUS       sequence-395851-       12313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1351
FEATURES             Location/Qualifiers
     source          1..12313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8170..8758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8223..8243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8641..8652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8830..9063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9141..9262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9178..9197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9232..9251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9302..9437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9457..9476)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9458..9476)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9486..9503)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9486..9502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9495..9517)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9644..10099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9731..9750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9941..9962
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10125..10229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10230..10298,10299..11090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10448..10467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11261..11849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11750..11769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12003..12020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12137..12158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12173..12178,12179..12196,12197..12203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12211..12227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12216..12238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12235..12251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12235..12251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12270..12290
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12272..12290
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacaccgacc tgttcatgga
     4141 atgtgaggag gaggagttgg agccatggca gaaaatcagt gatgtcattg aggactctgt
     4201 agttgaagat tataattcag tggataaaac taccacagtt tctgtgagcc agcagccagt
     4261 ctcggctcca gtgcccatcg ctgcccatgc ttctgttgct gggcacctct ctacatccac
     4321 caccgttagt agcagcgggg cacagaacag cgacagtaca aagaagactc ttgtcacact
     4381 aattgccaac aacaatgccc caggtaccca gtttgttaag ccgacagttg gagttccaca
     4441 agtgttctcc cagatgaccc ctgtgaggcc aggctccaca atgcctgtga ggcccaccac
     4501 caacaccttc accaccgtca tcccggccac tcttaccatt cgaagcaccg tcccacagtc
     4561 ccagtcccag cagaccaagt ccactcccag cacttctacc actcccactg ccacacagcc
     4621 aacctcactg gggcaactag ctgttcagtc tccaggccag tcaaaccaga ccacgaatcc
     4681 caagctagct ccctccttcc cctctccacc tgcagtgagc attgccagct ttgtcactgt
     4741 gaagcgacct ggtgttacag gcgaaaatag caatgaagtg gccaaattgg tgaataccct
     4801 taacaccatc ccttccctgg gccagagtcc tgggccagtg gtggtgtcca acaacagctc
     4861 tgctcatggc tctcaaagaa ccagcggacc tgagtcttca atgaaagtga cctcttccat
     4921 cccagtattt gacctccagg atggtggacg gaaaatatgt ccacgatgta atgctcaatt
     4981 tcgtgttact gaagctttga gaggtcacat gtgttactgt tgcccagaaa tggttgaata
     5041 ccagaagaaa ggaaagtccc tggattcaga acccagtgtc ccatcagcag caaagccccc
     5101 atcccctgag aaaacagctc ctgttgcttc cacaccctct tctacaccta ttcctgctct
     5161 gtcaccgcct accaaagtac cagaaccaaa tgagaacgtg ggcgatgccg tccagaccaa
     5221 actcattatg cttgtagatg acttctacta tggacgggat ggtggcaaag tagcccagct
     5281 cacaaatttc cctaaggtcg ccacatcttt ccgatgccca cattgtacca aaaggctaaa
     5341 aaacaatatt cgattcatga accatatgaa acaccacgta gaactcgatc agcagaacgg
     5401 tgaggtagat ggtcacacta tctgccagca ctgttaccgc cagttttcca ctcccttcca
     5461 gcttcagtgc cacttggaaa atgttcatag tccctatgaa tctactacca agtgcaagat
     5521 ctgtgaatgg gcgtttgaaa gtgagccact atttctccag catatgaagg atactcataa
     5581 gcctggagag atgccttatg tttgccaggt gtgtcaatat cgctcctcac tctactctga
     5641 ggtagatgtc cattttcgga tgatccatga ggatacccgg catctgctct gcccttattg
     5701 cctgaaggtc ttcaaaaatg gcaatgcatt ccaacagcat tacatgaggc accagaagag
     5761 aaatgtttat cactgcaaca aatgccggct gcagtttctc tttgccaagg acaaaattga
     5821 acacaagctt caacaccata aaaccttccg taaacccaag cagctggagg gcttgaaacc
     5881 aggcaccaag gtgacaatcc gggcttcccg agggcagcca cgaactgttc ctgtatcctc
     5941 taatgataca cctcccagcg ccttgcagga ggcagcaccg ctgacctcct caatggaccc
     6001 tctgcctgtc ttcctttatc cccctgtcca gcgcagcatc cagaagagag ctgttaggaa
     6061 aatgagtgtc atgggccggc agacatgcct ggagtgcagc ttcgagatcc cagacttccc
     6121 taatcatttc cctacttacg tacactgctc tctgtgtcgc tatagcacct gctgttctcg
     6181 agcttatgcc aaccacatga tcaacaatca tgttccacgg aagagcccca agtatttggc
     6241 tttgtttaaa aattctgtga gtggaatcaa gctggcctgc acttcatgta cctttgttac
     6301 ctctgtgggc gatgctatgg ccaagcattt ggtattcaac ccctctcaca gatccagcag
     6361 catcctgcca cggggactca cttggatagc tcactcaagg catggccaga ctcgtgaccg
     6421 agtgcatgac cggaacgtga agaatatgta ccctcctcct tccttcccca ctaacaaagc
     6481 tgccactgtg aaatctgcgg gggccacccc agctgagcct gaagagctac taactccctt
     6541 agccccagca ctcccatcac cagcctcaac tgcaacccca ccaccaaccc ccactcaccc
     6601 gcaggcttta gcccttccac cgctggctac agagggagcc gaatgtctga atgttgatga
     6661 tcaggatgaa gggagcccag tcacccaaga acctgagcta gcatcaggtg gtggtggtag
     6721 tggtggagtt ggcaaaaagg agcagctgtc tgtgaagaag cttcgagtag tactgtttgc
     6781 tctatgctgc aatacagaac aggcagctga acacttccga aatccccagc gacgtattcg
     6841 ccgttggctt cgacgtttcc aggcctccca gggggagaat ctagagggca aatatctgag
     6901 ctttgaggca gaagagaaac tggctgagtg ggtgctaacc cagcgcgaac aacagctacc
     6961 tgtaaatgag gagaccttgt tccagaaggc caccaaaata ggacgttctt tggaaggggg
     7021 gtttaagatc tcctatgagt gggctgtgcg tttcatgctg cggcaccacc tgactcccca
     7081 tgcccggcga gctgtggccc acaccctacc taaggatgta gcagagaatg caggactctt
     7141 cattgatttt gtacaacggc agattcacaa ccaggactta cccttgtcta tgattgtggc
     7201 tattgatgag atctctttgt tcctggatac agaggtgctg agcagtgatg atcgaaagga
     7261 gaatgccctg cagacagtgg gcacagggga accttggtgt gatgtagtcc tagccattct
     7321 ggcagatggc actgtccttc ccaccctggt tttctacaga gggcagatgg atcagcctgc
     7381 taacatgcca gactccatat tgctagaggc aaaggagagt ggctacagtg atgacgagat
     7441 catggagctg tggtcaactc gagtgtggca gaagcacaca gcttgccagc gcagcaaagg
     7501 catgcttgtg atggactgtc atcgcactca cttgtcagaa gaggtactgg ctatgcttag
     7561 tgcctctagc actttgcctg cagtggtccc agcaggctgt agctccaaaa ttcagccatt
     7621 agatgtatgc atcaaaagaa ctgtcaagaa cttcctgcat aaaaaatgga aggaacaggc
     7681 tcgggaaatg gcagatactg catgtgattc tgatgtcctg cttcagctgg tgcttgtctg
     7741 gctgggtgaa gtgctaggtg tcattgggga ctgtccagag ctagttcagc gctccttcct
     7801 ggtggctagt gttctgcctg gccccgatgg caacattaac tcacctacaa gaaatgctga
     7861 catgcaggag gagctaattg cctccctaga ggagcaactg aagctgagtg gggaacattc
     7921 tgagtcttcc actccacgac ccagatcatc tcctgaagag acaattgagc ctgaaagtct
     7981 tcaccagctc tttgagggtg aaagtgagac cgagtctttc tatggctttg aagaagctga
     8041 cctagatctg atggagattt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     8101 ttggctttat atatcttgtg gaaaggacga tgacaagttg gtgtctagaa ggcgacgcgt
     8161 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8221 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8281 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8341 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8401 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8461 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     8521 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     8581 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     8641 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     8701 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     8761 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8821 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8881 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8941 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9001 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9061 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9121 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     9181 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9241 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     9301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9361 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9421 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     9481 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9541 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9601 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     9661 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9721 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9781 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9841 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9901 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9961 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10021 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    10081 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10141 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    10201 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    10261 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    10321 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    10381 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    10441 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    10501 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    10561 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10621 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10681 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10741 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10801 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10861 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10921 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    10981 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    11041 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    11101 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    11161 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    11221 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    11281 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    11341 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    11401 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    11461 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11521 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11581 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11641 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11701 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11761 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11821 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11881 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11941 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    12001 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    12061 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    12121 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    12181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    12241 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    12301 tggagctgca agc
//
