LOCUS       sequence-395852-       12412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1353
FEATURES             Location/Qualifiers
     source          1..12412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8269..8857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8322..8342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8740..8751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8929..9162
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9240..9361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9277..9296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9331..9350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9401..9536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9556..9575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9557..9575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9585..9602)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9585..9601)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9594..9616)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9743..10198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9830..9849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10040..10061
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10224..10328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10329..10397,10398..11189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10547..10566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11360..11948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11849..11868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12102..12119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12236..12257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12272..12277,12278..12295,12296..12302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12310..12326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12315..12337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12334..12350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12334..12350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12369..12389
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12371..12389
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacaccgacc tgttcatgga
     4141 atgtgaggag gaggagttgg agccatggca gaaaatcagt gatgtcattg aggactctgt
     4201 agttgaagat tataattcag tggataaaac taccacagct ggcaatcctt tggtccagca
     4261 aggtggacag ccactcatcc tgacccagaa tccagcccca ggtctgggca caatggttac
     4321 tcaaccagta ttgaggcctg ttcaggtcat gcagaatgcc aatcatgtga ctagttcccc
     4381 tgtggcctca caaccaatat ttatcactac gcagggattt cctgtaagga atgtccggcc
     4441 tgtacaaaat gcaatgaatc aggttgggat tgtgctgaac gtacagcaag gccaaacggt
     4501 tagaccaatt acactagttc cagccccagg tacccagttt gttaagccga cagttggagt
     4561 tccacaagtg ttctcccaga tgacccctgt gaggccaggc tccacaatgc ctgtgaggcc
     4621 caccaccaac accttcacca ccgtcatccc ggccactctt accattcgaa gcaccgtccc
     4681 acagtcccag tcccagcaga ccaagtccac tcccagcact tctaccactc ccactgccac
     4741 acagccaacc tcactggggc aactagctgt tcagtctcca ggccagtcaa accagaccac
     4801 gaatcccaag ctagtgagca ttgccagctt tgtcactgtg aagcgacctg gtgttacagg
     4861 cgaaaatagc aatgaagtgg ccaaattggt gaataccctt aacaccatcc cttccctggg
     4921 ccagagtcct gggccagtgg tggtgtccaa caacagctct gctcatggct ctcaaagaac
     4981 cagcggacct gagtcttcaa tgaaagtgac ctcttccatc ccagtatttg acctccagga
     5041 tggtggacgg aaaatatgtc cacgatgtaa tgctcaattt cgtgttactg aagctttgag
     5101 aggtcacatg tgttactgtt gcccagaaat ggttgaatac cagaagaaag gaaagtccct
     5161 ggattcagaa cccagtgtcc catcagcagc aaagccccca tcccctgaga aaacagctcc
     5221 tgttgcttcc acaccctctt ctacacctat tcctgctctg tcaccgccta ccaaagtacc
     5281 agaaccaaat gagaacgtgg gcgatgccgt ccagaccaaa ctcattatgc ttgtagatga
     5341 cttctactat ggacgggatg gtggcaaagt agcccagctc acaaatttcc ctaaggtcgc
     5401 cacatctttc cgatgcccac attgtaccaa aaggctaaaa aacaatattc gattcatgaa
     5461 ccatatgaaa caccacgtag aactcgatca gcagaacggt gaggtagatg gtcacactat
     5521 ctgccagcac tgttaccgcc agttttccac tcccttccag cttcagtgcc acttggaaaa
     5581 tgttcatagt ccctatgaat ctactaccaa gtgcaagatc tgtgaatggg cgtttgaaag
     5641 tgagccacta tttctccagc atatgaagga tactcataag cctggagaga tgccttatgt
     5701 ttgccaggtg tgtcaatatc gctcctcact ctactctgag gtagatgtcc attttcggat
     5761 gatccatgag gatacccggc atctgctctg cccttattgc ctgaaggtct tcaaaaatgg
     5821 caatgcattc caacagcatt acatgaggca ccagaagaga aatgtttatc actgcaacaa
     5881 atgccggctg cagtttctct ttgccaagga caaaattgaa cacaagcttc aacaccataa
     5941 aaccttccgt aaacccaagc agctggaggg cttgaaacca ggcaccaagg tgacaatccg
     6001 ggcttcccga gggcagccac gaactgttcc tgtatcctct aatgatacac ctcccagcgc
     6061 cttgcaggag gcagcaccgc tgacctcctc aatggaccct ctgcctgtct tcctttatcc
     6121 ccctgtccag cgcagcatcc agaagagagc tgttaggaaa atgagtgtca tgggccggca
     6181 gacatgcctg gagtgcagct tcgagatccc agacttccct aatcatttcc ctacttacgt
     6241 acactgctct ctgtgtcgct atagcacctg ctgttctcga gcttatgcca accacatgat
     6301 caacaatcat gttccacgga agagccccaa gtatttggct ttgtttaaaa attctgtgag
     6361 tggaatcaag ctggcctgca cttcatgtac ctttgttacc tctgtgggcg atgctatggc
     6421 caagcatttg gtattcaacc cctctcacag atccagcagc atcctgccac ggggactcac
     6481 ttggatagct cactcaaggc atggccagac tcgtgaccga gtgcatgacc ggaacgtgaa
     6541 gaatatgtac cctcctcctt ccttccccac taacaaagct gccactgtga aatctgcggg
     6601 ggccacccca gctgagcctg aagagctact aactccctta gccccagcac tcccatcacc
     6661 agcctcaact gcaaccccac caccaacccc cactcacccg caggctttag cccttccacc
     6721 gctggctaca gagggagccg aatgtctgaa tgttgatgat caggatgaag ggagcccagt
     6781 cacccaagaa cctgagctag catcaggtgg tggtggtagt ggtggagttg gcaaaaagga
     6841 gcagctgtct gtgaagaagc ttcgagtagt actgtttgct ctatgctgca atacagaaca
     6901 ggcagctgaa cacttccgaa atccccagcg acgtattcgc cgttggcttc gacgtttcca
     6961 ggcctcccag ggggagaatc tagagggcaa atatctgagc tttgaggcag aagagaaact
     7021 ggctgagtgg gtgctaaccc agcgcgaaca acagctacct gtaaatgagg agaccttgtt
     7081 ccagaaggcc accaaaatag gacgttcttt ggaagggggg tttaagatct cctatgagtg
     7141 ggctgtgcgt ttcatgctgc ggcaccacct gactccccat gcccggcgag ctgtggccca
     7201 caccctacct aaggatgtag cagagaatgc aggactcttc attgattttg tacaacggca
     7261 gattcacaac caggacttac ccttgtctat gattgtggct attgatgaga tctctttgtt
     7321 cctggataca gaggtgctga gcagtgatga tcgaaaggag aatgccctgc agacagtggg
     7381 cacaggggaa ccttggtgtg atgtagtcct agccattctg gcagatggca ctgtccttcc
     7441 caccctggtt ttctacagag ggcagatgga tcagcctgct aacatgccag actccatatt
     7501 gctagaggca aaggagagtg gctacagtga tgacgagatc atggagctgt ggtcaactcg
     7561 agtgtggcag aagcacacag cttgccagcg cagcaaaggc atgcttgtga tggactgtca
     7621 tcgcactcac ttgtcagaag aggtactggc tatgcttagt gcctctagca ctttgcctgc
     7681 agtggtccca gcaggctgta gctccaaaat tcagccatta gatgtatgca tcaaaagaac
     7741 tgtcaagaac ttcctgcata aaaaatggaa ggaacaggct cgggaaatgg cagatactgc
     7801 atgtgattct gatgtcctgc ttcagctggt gcttgtctgg ctgggtgaag tgctaggtgt
     7861 cattggggac tgtccagagc tagttcagcg ctccttcctg gtggctagtg ttctgcctgg
     7921 ccccgatggc aacattaact cacctacaag aaatgctgac atgcaggagg agctaattgc
     7981 ctccctagag gagcaactga agctgagtgg ggaacattct gagtcttcca ctccacgacc
     8041 cagatcatct cctgaagaga caattgagcc tgaaagtctt caccagctct ttgagggtga
     8101 aagtgagacc gagtctttct atggctttga agaagctgac ctagatctga tggagattta
     8161 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     8221 aaaggacgag ttgttatcga ccttggcaca ttcacgcgtt aagtcgacaa tcaacctctg
     8281 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8341 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8401 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8461 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8521 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8581 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8641 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8701 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8761 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8821 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8881 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8941 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     9001 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9061 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9121 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     9181 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     9241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9361 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     9421 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9481 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9541 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     9601 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9661 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9721 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9901 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9961 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10021 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10081 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    10141 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    10201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10321 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10381 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10441 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10501 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10561 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10621 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10681 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10741 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10801 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10861 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10921 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10981 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11041 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11101 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11161 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12001 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12061 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    12241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    12301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    12361 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
