LOCUS       sequence-395919-       11758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2302
FEATURES             Location/Qualifiers
     source          1..11758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4181..4190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7615..8203
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7668..7688)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8086..8097)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8275..8508
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8586..8707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8623..8642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8677..8696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8747..8882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8902..8921)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8903..8921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8931..8948)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8931..8947)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8940..8962)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9089..9544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9176..9195)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9386..9407
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9570..9674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9675..9743,9744..10535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9893..9912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10706..11294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11195..11214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11448..11465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11582..11603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11618..11623,11624..11641,11642..11648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11656..11672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11661..11683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11680..11696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11680..11696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11715..11735
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11717..11735
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcgt tgcctggctc cacgccctgc
     4141 tgggtcctac ctgtcagagc cccaaggcag ctcacagtgt gccaccatgg agttggggcc
     4201 cctagaaggt ggctacctgg agcttcttaa cagcgatgct gaccccctgt gcctctacca
     4261 cttctatgac cagatggacc tggctggaga agaagagatt gagctctact cagaacccga
     4321 cacagacacc atcaactgcg accagttcag caggctgttg tgtgacatgg aaggtgatga
     4381 agagaccagg gaggcttatg ccaatatcgc ggaactggac cagtatgtct tccaggactc
     4441 ccagctggag ggcctgagca aggacatttt caagcacata ggaccagatg aagtgatcgg
     4501 tgagagtatg gagatgccag cagaagttgg gcagaaaagt cagaaaagac ccttcccaga
     4561 ggagcttccg gcagacctga agcactggaa gccagctgag ccccccactg tggtgactgg
     4621 cagtctccta gtgggaccag tgagcgactg ctccaccctg ccctgcctgc cactgcctgc
     4681 gctgttcaac caggagccag cctccggcca gatgcgcctg gagaaaaccg accagattcc
     4741 catgcctttc tccagttcct cgttgagctg cctgaatctc cctgagggac ccatccagtt
     4801 tgtccccacc atctccactc tgccccatgg gctctggcaa atctctgagg ctggaacagg
     4861 ggtctccagt atattcatct accatggtga ggtgccccag gccagccaag taccccctcc
     4921 cagtggattc actgtccacg gcctcccaac atctccagac cggccaggct ccaccagccc
     4981 cttcgctcca tcagccactg acctgcccag catgcctgaa cctgccctga cctcccgagc
     5041 aaacatgaca gagcacaaga cgtcccccac ccaatgcccg gcagctggag aggtctccaa
     5101 caagcttcca aaatggcctg agccggtgga gcagttctac cgctcactgc aggacacgta
     5161 tggtgccgag cccgcaggcc cggatggcat cctagtggag gtggatctgg tgcaggccag
     5221 gctggagagg agcagcagca agagcctgga gcgggaactg gccaccccgg actgggcaga
     5281 acggcagctg gcccaaggag gcctggctga ggtgctgttg gctgccaagg agcaccggcg
     5341 gccgcgtgag acacgagtga ttgctgtgct gggcaaagct ggtcagggca agagctattg
     5401 ggctggggca gtgagccggg cctgggcttg tggccggctt ccccagtacg actttgtctt
     5461 ctctgtcccc tgccattgct tgaaccgtcc gggggatgcc tatggcctgc aggatctgct
     5521 cttctccctg ggcccacagc cactcgtggc ggccgatgag gttttcagcc acatcttgaa
     5581 gagacctgac cgcgttctgc tcatcctaga cggcttcgag gagctggaag cgcaagatgg
     5641 cttcctgcac agcacgtgcg gaccggcacc ggcggagccc tgctccctcc gggggctgct
     5701 ggccggcctt ttccagaaga agctgctccg aggttgcacc ctcctcctca cagcccggcc
     5761 ccggggccgc ctggtccaga gcctgagcaa ggccgacgcc ctatttgagc tgtccggctt
     5821 ctccatggag caggcccagg catacgtgat gcgctacttt gagagctcag ggatgacaga
     5881 gcaccaagac agagccctga cgctcctccg ggaccggcca cttcttctca gtcacagcca
     5941 cagccctact ttgtgccggg cagtgtgcca gctctcagag gccctgctgg agcttgggga
     6001 ggacgccaag ctgccctcca cgctcacggg actctatgtc ggcctgctgg gccgtgcagc
     6061 cctcgacagc ccccccgggg ccctggcaga gctggccaag ctggcctggg agctgggccg
     6121 cagacatcaa agtaccctac aggaggacca gttcccatcc gcagacgtga ggacctgggc
     6181 gatggccaaa ggcttagtcc aacacccacc gcgggccgca gagtccgagc tggccttccc
     6241 cagcttcctc ctgcaatgct tcctgggggc cctgtggctg gctctgagtg gcgaaatcaa
     6301 ggacaaggag ctcccgcagt acctagcatt gaccccaagg aagaagaggc cctatgacaa
     6361 ctggctggag ggcgtgccac gctttctggc tgggctgatc ttccagcctc ccgcccgctg
     6421 cctgggagcc ctactcgggc catcggcggc tgcctcggtg gacaggaagc agaaggtgct
     6481 tgcgaggtac ctgaagcggc tgcagccggg gacactgcgg gcgcggcagc tgctggagct
     6541 gctgcactgc gcccacgagg ccgaggaggc tggaatttgg cagcacgtgg tacaggagct
     6601 ccccggccgc ctctcttttc tgggcacccg cctcacgcct cctgatgcac atgtactggg
     6661 caaggccttg gaggcggcgg gccaagactt ctccctggac ctccgcagca ctggcatttg
     6721 cccctctgga ttggggagcc tcgtgggact cagctgtgtc acccgtttca gggctgcctt
     6781 gagcgacacg gtggcgctgt gggagtccct gcagcagcat ggggagacca agctacttca
     6841 ggcagcagag gagaagttca ccatcgagcc tttcaaagcc aagtccctga aggatgtgga
     6901 agacctggga aagcttgtgc agactcagag gacgagaagt tcctcggaag acacagctgg
     6961 ggagctccct gctgttcggg acctaaagaa actggagttt gcgctgggcc ctgtctcagg
     7021 cccccaggct ttccccaaac tggtgcggat cctcacggcc ttttcctccc tgcagcatct
     7081 ggacctggat gcgctgagtg agaacaagat cggggacgag ggtgtctcgc agctctcagc
     7141 caccttcccc cagctgaagt ccttggaaac cctcaatctg tcccagaaca acatcactga
     7201 cctgggtgcc tacaaactcg ccgaggccct gccttcgctc gctgcatccc tgctcaggct
     7261 aagcttgtac aataactgca tctgcgacgt gggagccgag agcttggctc gtgtgcttcc
     7321 ggacatggtg tccctccggg tgatggacgt ccagtacaac aagttcacgg ctgccggggc
     7381 ccagcagctc gctgccagcc ttcggaggtg tcctcatgtg gagacgctgg cgatgtggac
     7441 gcccaccatc ccattcagtg tccaggaaca cctgcaacaa caggattcac ggatcagcct
     7501 gagatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     7561 ttgtggaaag gacgaaattc tcatactatc cgctgtcgaa cgcgttaagt cgacaatcaa
     7621 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7681 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7741 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7801 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7861 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7921 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7981 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8041 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8101 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8161 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8221 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8281 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     8521 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8581 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8701 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8761 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8821 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8881 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8941 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9001 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9061 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9121 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9181 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9241 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9301 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9361 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9421 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9481 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9541 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11581 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11641 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11701 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
