LOCUS       sequence-395942-       11728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0137
FEATURES             Location/Qualifiers
     source          1..11728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7585..8173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7638..7658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8056..8067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8245..8478
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8556..8677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8593..8612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8647..8666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8717..8852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8872..8891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8873..8891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8901..8918)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8901..8917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8910..8932)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9059..9514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9146..9165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9356..9377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9540..9644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9645..9713,9714..10505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9863..9882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10676..11264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11165..11184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11418..11435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11552..11573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11588..11593,11594..11611,11612..11618)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11626..11642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11631..11653
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11650..11666
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11650..11666
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11685..11705
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11687..11705
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gaggcgcctc ctgtctcagg
     4141 tacttttaaa ttcaatacag atgctgctga attcattcct caggagaaaa aaaattctgg
     4201 tctaaattgt gggactcaaa ggagactaga ctctaatagg attggtagaa gaaattacag
     4261 ttcaccacct ccctgtcacc tttccaggca ggtcccttat gatgaaatct ctgctgttca
     4321 tcagcatagt tatcatccgt caggaagcaa acctaagagt cagcagacgt ctttccagtc
     4381 ctctccttgt aataaatcgc ccaagagcca tggccttcag aatcaacctt ggcagaaatt
     4441 gaggaatgag aagcaccata tcagagtcaa gaaagcacag agtcttgctg agcagacctc
     4501 agatacagct ggattagaga gctcgaccag atcagagagt gggacagacc tcagagagca
     4561 tagtccttct gagagtgaga aggaagttgt gggtgcagat cccaggggag caaaacccaa
     4621 aaaagcaaca cagtttgtat acagctatgg tagaggacca aaagtcaagg ggaaactcaa
     4681 atgtgaatgg agtaaccgaa caactccaaa accggaggat gctggacccg aaagtaccaa
     4741 acctgtgggg gttttccacc ctgactcttc agaggcatcc tctagaaaag gagtattgga
     4801 tgggtatgga gccagacgaa atgagcagag aagataccca cagaaaaggc ctccctggga
     4861 agtggagggg gccaggccac gaccaggcag aaatccacca aaacaggagg gccaccgaca
     4921 tacaaacgca ggacacagaa acaacatggg ccccattcca aaggatgacc tcaatgaaag
     4981 accagcaaaa tctacctgtg acagtgagaa cttggcagtc atcaacaagt cttccaggag
     5041 ggttgaccaa gagaaatgca ctgtacggag gcaggatcct caagtagtat ctcctttctc
     5101 ccgaggcaaa cagaaccatg tgctaaagaa tgtggaaacg cacacaggtt ctctaattga
     5161 acaactaaca acagaaaaat acgagtgcat ggtgtgctgt gaattggttc gtgtcacggc
     5221 cccagtgtgg agttgtcaga gctgttacca tgtgtttcat ttgaactgca taaagaaatg
     5281 ggcaaggtct ccagcatctc aagcagatgg ccagagtggt tggaggtgcc ctgcctgtca
     5341 gaatgtttct gcacatgttc ctaataccta cacttgtttc tgtggcaagg taaagaatcc
     5401 tgagtggagc agaaatgaaa ttccacatag ctgtggtgag gtttgtagaa agaaacagcc
     5461 tggccaggac tgcccacatt cctgtaacct tctctgccat ccaggaccct gcccaccctg
     5521 ccctgccttt atgacaaaaa catgtgaatg tggacgaacc aggcacacag ttcgctgtgg
     5581 tcaggctgtc tcagtccact gttctaaccc atgtgagaat attttgaact gtggtcagca
     5641 ccagtgtgct gagctgtgcc atgggggtca gtgccagcct tgccagatca ttttgaacca
     5701 ggtatgctat tgcggcagca cctcccgaga tgtgttatgt ggaaccgatg taggaaagtc
     5761 tgatggattt ggggatttca gctgtttaaa gatatgtggc aaggacttga aatgcggtaa
     5821 ccatacatgt tcgcaagtgt gccaccctca gccctgccag caatgcccac ggctccccca
     5881 gctggtgcgc tgttgcccct gtggccaaac tcctctcagc caattgctag aacttggaag
     5941 tagtagtcgg aaaacatgca tggaccctgt gccttcatgt ggaaaagtgt gcggcaagcc
     6001 tctgccttgt ggttccttag atttcattca tacctgtgaa aagctctgcc atgaaggaga
     6061 ctgtggacca tgctctcgca catcagttat ttcctgcaga tgctctttca gaacaaagga
     6121 gcttccatgt accagtctca aaagtgaaga tgctacattt atgtgtgaca agcggtgtaa
     6181 caagaaacgg ttgtgtggac ggcataaatg taatgagata tgctgtgtgg ataaggagca
     6241 caagtgtcct ttgatttgtg ggaggaaact ccgttgtggc cttcataggt gtgaagaacc
     6301 ttgtcatcgt ggaaactgcc agacatgctg gcaagccagt tttgatgaat taacctgcca
     6361 ttgtggtgca tcagtgattt accctccagt tccctgtggt actaggcccc ctgaatgtac
     6421 ccaaacctgc gctagagtcc atgagtgtga ccatccagta tatcattctt gtcatagtga
     6481 ggagaagtgt cccccttgca ctttcctaac tcagaagtgg tgcatgggca agcatgagtt
     6541 tcggagcaac atcccctgtc acctggttga tatctcttgc ggattaccct gcagtgccac
     6601 gctaccatgt gggatgcaca aatgtcagag actctgtcac aaaggggagt gtcttgtgga
     6661 tgagccctgc aagcagccct gcaccacccc cagagctgac tgtggtcacc cgtgtatggc
     6721 accctgccat accagctcac cctgccctgt gactgcttgt aaagctaagg tagagctaca
     6781 gtgtgaatgt ggacgaagaa aagagatggt gatttgctct gaagcatcta gtacttatca
     6841 aagaatagct gcaatctcca tggcctctaa gataacagac atgcagcttg gaggttcagt
     6901 ggagatcagc aagttaatta ccaaaaagga agttcatcaa gccaggctgg agtgtgatga
     6961 ggagtgttca gccttggaaa ggaaaaagag attagcagag gcatttcata tcagtgagga
     7021 ttctgatcct ttcaatatac gttcttcagg gtcaaaattc agtgatagtt tgaaagaaga
     7081 tgccaggaag gacttaaagt ttgtcagtga cgttgagaag gaaatggaaa ccctcgtgga
     7141 ggccgtgaat aagggaaaga atagtaagaa aagccacagc ttccctccca tgaacagaga
     7201 ccaccgccgg atcatccatg acttggccca agtttatggc ctggagagcg tgagctatga
     7261 cagtgaaccg aagcgcaatg tggtggtcac tgccatcagg gggaagtccg tttgtcctcc
     7321 taccacgctg acaggtgtgc ttgaaaggga aatgcaggca cggcctccac caccgattcc
     7381 tcatcacaga catcagtcag acaagaatcc tgggagcagt aatttacaga aaataaccaa
     7441 ggagccaata attgactatt ttgacgtcca ggactagtaa tgaactagtc cgtaacttga
     7501 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagtttg ccgttccgcg
     7561 acgttcctaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7621 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7681 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7741 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7801 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7861 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7921 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7981 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8041 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8101 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8161 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8221 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8281 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8341 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8401 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8461 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     8521 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8821 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8881 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8941 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     9001 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     9061 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9121 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9181 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9241 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9301 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9361 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9421 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9481 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     9541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9901 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9961 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10021 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10081 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10141 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10201 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10261 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10321 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10381 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10441 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10501 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10561 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10621 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10801 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11041 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11281 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11341 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11401 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11461 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11521 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11581 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11641 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    11701 aaagggaaca aaagctggag ctgcaagc
//
