LOCUS       sequence-395943-       12457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0195
FEATURES             Location/Qualifiers
     source          1..12457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8314..8902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8367..8387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8785..8796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8974..9207
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9285..9406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9322..9341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9376..9395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9446..9581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9601..9620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9602..9620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9630..9647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9630..9646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9639..9661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9788..10243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9875..9894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10085..10106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10269..10373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10374..10442,10443..11234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10592..10611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11405..11993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11894..11913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12147..12164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12281..12302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12317..12322,12323..12340,12341..12347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12355..12371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12360..12382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12379..12395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12379..12395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12414..12434
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12416..12434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtca tctagtcggg cacaacaaat
     4141 gcatgccttt tcctggattc ggaataccct agaggaacat ccggagactt cactgcccaa
     4201 acaggaagtc tatgatgagt acaagagcta ttgtgacaat cttggttacc atccattaag
     4261 tgctgctgat tttggaaaga tcatgaaaaa cgtctttcca aacatgaagg cacgtcgttt
     4321 gggcacaaga ggcaaatcta aatattgcta cagtggacta agaaaaaaag cttttgttca
     4381 tatgccaaca ctgcccaacc ttgactttca caaaactgga gatgggttgg aaggagctga
     4441 accttctggg cagcttcaaa atattgatga agaagttatc tcttctgctt gccgtcttgt
     4501 gtgtgagtgg gcccagaaag tgttaagcca accatttgac accgtcttgg aattagcccg
     4561 cttccttgta aaaagtcact atataggcac caagtcaatg gcagctctaa ctgtaatggc
     4621 agcagcacca gcaggaatga aaggaattac ccagccttct gcttttatac ctacagctga
     4681 aagtaattcc tttcagcctc aggtgaagac tttgccatct ccaattgatg ctaaacagca
     4741 gttgcaacgg aaaatccaga agaagcagca agaacagaaa ctacaatccc ctttgccagg
     4801 agaatctgca gcaaaaaagt cagaaagtgc tacaagcaat ggagtgacta atcttcctaa
     4861 tggaaatcct tcaatccttt ctcctcaacc tattggtatc gttgtggcag ctgtccctag
     4921 tcccattccg gtccagcgga ctaggcaatt ggtaacttca ccgagtccaa tgagttcttc
     4981 tgacggcaaa gttcttcccc tcaatgtaca ggtggtcact cagcacatgc agtctgtgaa
     5041 acaggcacca aagactcccc agaacgttcc agccagtcct ggtggggatc gttctgcccg
     5101 gcaccgttac cctcagatct tacccaaacc agcgaacacc agtgcactca ccattcgctc
     5161 tccaactact gtcctcttta ctagtagtcc catcaaaact gctgttgtac ccgcttcaca
     5221 catgagttct ctaaatgtgg tgaaaatgac aacaatatcc ctcacaccca gcaacagtaa
     5281 cacccctctt aaacattctg cctcagtcag cagtgctaca ggaacaacag aagaatcaag
     5341 gagtgttcca cagatcaaga atggttctgt cgtgtcgctt cagtctcctg ggtccaggag
     5401 cagcagtgcg gggggaacat ctgctgtgga agtcaaagtg gaacccgaaa catcatcaga
     5461 tgagcatcct gtacagtgcc aagagaactc tgatgaggct aaagctcccc agacacctag
     5521 tgcccttttg gggcagaaaa gtaatacaga cggagcactg cagaaacctt caaatgaagg
     5581 tgtcattgaa ataaaagcaa ctaaggtctg tgaccagagg accaaatgta aaagtcgctg
     5641 taatgaaatg ctgccaggca cgtcaacagg caataatcaa agcactatca ctctatcagt
     5701 tgcttctcag aacttaactt tcaccagcag cagctcacca cctaatggtg actcaatcaa
     5761 taaagaccct aaattatgca ctaaaagccc aagaaaacga ctgtcttcta cattgcagga
     5821 gacccaggtg cctcctgtaa agaaaccaat tgtggaacag ctttcagcag ctaccataga
     5881 agggcagaaa caaggcagtg ttaagaagga ccaaaaggtt ccacattcag ggaaaacaga
     5941 aggttcaaca gcaggtgctc agattcctag caaggtatca gtaaatgtca gttcacacat
     6001 aggagcaaat caacccttga attcctctgc ccttgttatc agtgattcag ctttggaaca
     6061 gcaaacaacc ccatcatcat ctccagatat aaaagtaaaa cttgaaggaa gtgtctttct
     6121 cttggacagt gattcaaagt cagttggcag ctttaatcca aatggatggc aacaaatcac
     6181 taaagattct gagtttatat ctgccagttg tgaacaacag caagatatca gtgttatgac
     6241 aattcctgag cactctgata tcaatgactt agagaaatct gtttgggaat tagaaggaat
     6301 gccacaggac acatatagcc agcagctaca tagccagata caggaatctt ctttaaatca
     6361 aatacaagca cattcttcag atcagttacc tctgcaatct gaactgaagg agtttgagcc
     6421 ttctgtttcc cagacaaatg aaagctactt tccttttgat gatgaactta cacaagatag
     6481 tattgtggaa gagctggtgc ttatggagca gcaaatgtca atgaacaatt ctcattctta
     6541 cggcaactgt ttgggaatga cccttcagag tcagtcagta actccaggag ctccaatgtc
     6601 atctcacact tccagcaccc acttctatca tccaatccac agcaatggca ctccaatcca
     6661 cacacccaca cccacaccca cacccactcc tactccaacc ccaaccccaa ccccgacatc
     6721 tgaaatgatt gctggatctc agagtctgtc acgggagagc ccttgctcca ggctagccca
     6781 gactacacct gtggatagtg ctttaggaag tagccgacat acacccattg gtactccaca
     6841 ttctaactgc agcagtagtg tcccccccag ccctgttgaa tgcaggaatc cgtttgcatt
     6901 cactccaata agctccagta tggcatatca tgacgccagc attgtctcaa gtagtcctgt
     6961 gaaaccgatg caaagaccca tggccacaca ccctgacaaa accaagcttg aatggatgaa
     7021 taatgggtat agtggggttg gtaattcatc agtttctggc catggtattc tcccaagcta
     7081 tcaggaacta gtggaagacc gtttcaggaa acctcatgct tttgctgtgc ctggacagtc
     7141 ttatcagtct caatccagac atcatgacac tcattttggt cgtttgactc ctgtctctcc
     7201 tgtgcagcat caaggtgcca ctgtaaataa caccaacaaa caggagggtt ttgcagtccc
     7261 tgcccctctt gataataaag gaactaattc atctgccagc agcaacttca gatgccggag
     7321 tgtgagccct gctgttcatc gccaacgtaa tcttagtgga agcaccctct atccagtatc
     7381 taatatccca cgatctaatg tgaccccctt tggaagtcca gttaccccag aagttcatgt
     7441 tttcacaaat gttcacacag acgcatgtgc caacaacata gctcaaagaa gccaatcagt
     7501 tccattgaca gtcatgatgc agacagcctt cccaaacgct cttcagaagc aagcaaacag
     7561 taaaaaaata accaatgttt tgttgagtaa acttgattcc gacaatgatg atgcagtgag
     7621 aggtttggga atgaacaacc tgccctctaa ttatacagcc cggatgaatc tcactcagat
     7681 tttggaacct tccactgttt ttcctagtgc caacccacaa aatatgatcg attccagcac
     7741 ttctgtttat gagttccaaa caccatctta cctcaccaaa agtaatagca ccggtcagat
     7801 caatttttct cctggagata atcaagcaca atcagaaatt ggagagcaac aattagattt
     7861 caatagcact gttaaagacc tgttgagtgg agacagcttg caaaccaacc agcagctggt
     7921 aggtcaggga gcatctgatc tcactaatac tgcatctgat ttctctagcg atatcaggtt
     7981 gtcttctgag ctctcaggca gcatcaatga tttgaacact ttagacccaa atctactgtt
     8041 tgatccaggt cgtcagcagg gacaagatga tgaagctaca ctggaagaat taaagaatga
     8101 cccattattt caacaaattt gcagtgaatc catgaattct atgacttcat caggttttga
     8161 atggatagaa agcaaggacc atcctactgt tgaaatgttg ggttagtaat gaactagtcc
     8221 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgattcgct
     8281 tagcagatgc agtgctacac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     8341 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8401 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8461 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8521 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8581 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8641 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8701 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8761 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8821 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8881 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     8941 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9001 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9181 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     9241 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     9301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     9421 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     9601 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     9661 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9721 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9781 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10201 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    10261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    10501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    10561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    10621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    10681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    11221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    11281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    11341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    11461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    11521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    11581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    11641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    11701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    12121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    12181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    12241 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    12301 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    12361 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    12421 accctcacta aagggaacaa aagctggagc tgcaagc
//
