LOCUS       sequence-395965-       11674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0219
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7531..8119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7584..7604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8002..8013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8191..8424
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8502..8623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8539..8558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8593..8612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8663..8798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8818..8837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8819..8837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8847..8864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8847..8863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8856..8878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9005..9460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9092..9111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9302..9323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9486..9590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9591..9659,9660..10451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9809..9828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10622..11210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11111..11130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11364..11381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11498..11519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11534..11539,11540..11557,11558..11564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11572..11588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11577..11599
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11596..11612
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11596..11612
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11631..11651
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11633..11651
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgttc caacttcctg tcaacaatct
     4141 tggcagttta agaaaagccc ggaaaactgt gaaaaaaata cttagtgaca ttgggttgga
     4201 atactgtaaa gaacatatag aagattttaa acaatttgaa cctaatgact tttatttgaa
     4261 aaacactaca tgggaggatg taggactgtg ggacccatca cttacgaaaa accaggacta
     4321 tcggacaaaa cctttctgct gcagcgcttg tccattttcc tcaaaattct tctctgccta
     4381 caaaagtcat ttccgcaatg tccatagtga agactttgaa aataggattc tccttaattg
     4441 cccctactgt accttcaatg cagacaaaaa gactttggaa acacacatta aaatatttca
     4501 tgctccgaac gccagcgcac caagtagcag cctcagcact ttcaaagata aaaacaaaaa
     4561 tgatggcctt aaacctaagc aggctgacag tgtagagcaa gctgtttatt actgtaagaa
     4621 gtgcacttac cgagatcctc tttatgaaat agttaggaag cacatttaca gggaacattt
     4681 tcagcatgtg gcagcacctt acatagcaaa ggcaggagaa aaatcactca atggggcagt
     4741 ccccttaggc tcgaatgccc gagaagagag tagtattcac tgcaagcgat gccttttcat
     4801 gccaaagtcc tatgaagctt tggtacagca tgtcatcgaa gaccatgaac gtataggcta
     4861 tcaggtcact gccatgattg ggcacacaaa tgtagtggtt ccccgatcca aacccttgat
     4921 gctaattgct cccaaacctc aagacaagaa gagcatggga ctcccaccaa ggatcggttc
     4981 ccttgcttct ggaaatgtcc ggtctttacc atcacagcag atggtgaatc gactctcaat
     5041 accaaagcct aacttaaatt ctacaggagt caacatgatg tccagtgttc atctgcagca
     5101 gaacaactat ggagtcaaat ctgtaggcca gggttacagt gttggtcagt caatgagact
     5161 gggtctaggt ggcaacgcac cagtttccat tcctcaacaa tctcagtctg taaagcagtt
     5221 acttccaagt ggaaacggaa ggtcttatgg gcttgggtca gagcagaggt cccaggcacc
     5281 agcaagatac tccctgcagt ctgctaatgc ctcttctctc tcatcgggcc agttaaagtc
     5341 tccttccctc tctcagtcac aggcatccag agtgttaggt cagtccagtt ccaaacctgc
     5401 tgcagctgcc acaggccctc ccccaggtaa cacttcctca actcaaaagt ggaaaatatg
     5461 tacaatctgt aatgagcttt ttcctgaaaa tgtctatagt gtgcacttcg aaaaagaaca
     5521 taaagctgag aaagtcccag cagtagccaa ctacattatg aaaatacaca attttactag
     5581 caaatgcctc tactgtaatc gctatttacc cacagatact ctgctcaacc atatgttaat
     5641 tcatggtctg tcttgtccat attgccgttc aactttcaat gatgtggaaa agatggccgc
     5701 acacatgcgg atggttcaca ttgatgaaga gatgggacct aaaacagatt ctactttgag
     5761 ttttgatttg acattgcagc agggtagtca cactaacatc catctcctgg taactacata
     5821 caatctgagg gatgccccag ctgaatctgt tgcttaccat gcccaaaata atcctccagt
     5881 tcctccaaag ccacagccaa aggttcagga aaaggcagat atccctgtaa aaagttcacc
     5941 tcaagctgca gtgccctata aaaaagatgt tgggaaaacc ctttgtcctc tttgcttttc
     6001 aatcctaaaa ggacccatat ctgatgcact tgcacatcac ttacgagaga ggcaccaagt
     6061 tattcagacg gttcatccag ttgagaaaaa gctcacctac aaatgtatcc attgccttgg
     6121 tgtgtatacc agcaacatga ccgcctcaac tatcactctg catctagttc actgcagggg
     6181 cgttggaaag acccaaaatg gccaggataa gacaaatgca ccctctcggc ttaatcagtc
     6241 tccaagtctg gcacctgtga agcgcactta cgagcaaatg gaatttccct tactgaaaaa
     6301 acgaaagtta gatgatgata gtgattcacc cagcttcttt gaagagaagc ctgaagagcc
     6361 tgttgtttta gctttagacc ccaagggtca tgaagatgat tcctatgaag ccaggaaaag
     6421 ctttctaaca aagtatttca acaaacagcc ctatcccacc aggagagaaa ttgagaagct
     6481 agcagccagt ttatggttat ggaagagtga catcgcttcc cattttagta acaaaaggaa
     6541 gaagtgtgtc cgtgattgtg aaaagtacaa gcctggcgtg ttgctggggt ttaacatgaa
     6601 agaattaaat aaagtcaagc atgagatgga ttttgatgct gagtggctat ttgaaaatca
     6661 tgatgagaag gattccagag tcaatgctag taagactgct gacaaaaagc tcaaccttgg
     6721 gaaggaagat gacagttcct cagacagttt tgaaaatttg gaagaagaat ccaatgaaag
     6781 tggtagccct tttgaccctg tttttgaagt tgaacctaaa atctctaacg ataacccaga
     6841 ggaacatgta ctgaaggtaa ttcctgagga tgcttcagaa tctgaggaga agctagacca
     6901 aaaagaggat ggttcaaaat acgaaactat tcatttgact gaggaaccaa ccaaactaat
     6961 gcacaatgca tctgatagtg aggttgacca agacgatgtt gttgagtgga aagacggtgc
     7021 ttctccatct gagagtgggc ctggatccca acaagtgtca gactttgagg acaatacctg
     7081 cgaaatgaaa ccaggaacct ggtctgacga gtcttcccaa agcgaagatg caaggagcag
     7141 taagccagct gccaaaaaaa aggctaccat gcaaggtgac agagagcagt tgaaatggaa
     7201 gaatagttcc tatggaaaag ttgaagggtt ttggtctaag gaccagtcac agtggaagaa
     7261 tgcatctgag aatgatgagc gcttatctaa cccccagatt gagtggcaga atagcacaat
     7321 tgacagtgag gatggggaac agtttgacaa catgactgat ggagtagctg agcccatgca
     7381 tggcagctta gccggagtta aactgagcag ccaacaggcc tagtaatgaa ctagtccgta
     7441 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg accggcgctt
     7501 aacggctaat actggacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     7561 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7621 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7681 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7741 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7801 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7861 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7921 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7981 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8041 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8101 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     8161 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8221 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8281 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8341 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8401 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     8461 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8581 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8641 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8701 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8761 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     8821 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8881 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8941 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9001 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9421 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    11401 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    11461 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11521 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11581 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    11641 ctcactaaag ggaacaaaag ctggagctgc aagc
//
