LOCUS       sequence-395971-       11959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0245
FEATURES             Location/Qualifiers
     source          1..11959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7816..8404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7869..7889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8287..8298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8476..8709
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8787..8908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8824..8843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8878..8897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8948..9083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9103..9122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9104..9122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9132..9149)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9132..9148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9141..9163)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9290..9745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9377..9396)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9587..9608
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9771..9875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9876..9944,9945..10736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10094..10113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10907..11495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11396..11415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11649..11666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11783..11804
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11819..11824,11825..11842,11843..11849)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11857..11873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11862..11884
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11881..11897
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11881..11897
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11916..11936
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11918..11936
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc gctgccgccg tcacccgcgg
     4141 gaccccggga gaaaacagcc accacctgaa gatctttctc cccaagaagc tgctggagtg
     4201 tcttcctcgc tgcccgctgc tgcctccaga gaggctacgg tggaatacaa atgaggagat
     4261 tgcatcctac ctgatcacct ttgagaagca tgatgagtgg ctgtcttgtg ccccaaagac
     4321 aaggcctcag aatggctcca tcatcctcta caatcgcaag aaggtgaaat atcggaagga
     4381 tggttacctc tggaagaagc ggaaggatgg gaagaccacc cgagaggacc acatgaagct
     4441 gaaggtccag ggcatggagt gtctctatgg ctgctacgtt cactcttcca tcgtccccac
     4501 attccatcgg cgctgctact ggctgctcca gaaccctgac atcgtccttg tgcactacct
     4561 gaacgtccca gccctggagg actgtggaaa gggctgcagc cccatctttt gttccatcag
     4621 cagcgaccgt cgagagtggc tgaagtggtc ccgggaggag ttgttgggac agctgaagcc
     4681 catgtttcat ggcatcaagt ggagctgcgg gaatggaaca gaagagttct ctgtagaaca
     4741 cctggtgcag cagattttgg acacccaccc aaccaagcct gctccccgaa cccacgcctg
     4801 tctctgcagt ggggggcttg gttctgggag ccttacccac aaatgcagca gcacgaaaca
     4861 ccgcatcatc tctcccaaag tggagccccg agctttaacc ctgacctcta tcccccacgc
     4921 tcacccccca gagcctcctc cactgatagc cccacttccc ccagagctcc ccaaggcaca
     4981 cacctcccca tcttcttcct cttcttcctc ctcatcaggt tttgcagagc ccctagaaat
     5041 cagacctagc cctcccactt ctcgaggggg ttcttcaaga ggaggcactg ctatcctcct
     5101 cctgacagga ctggagcagc gggctggagg cttgacgccc accaggcact tggctccaca
     5161 ggctgatcct aggccttcca tgagtttggc agtggttgta ggcactgagc cttctgcccc
     5221 accagctcct cccagtcctg cctttgaccc tgatcgtttt ctcaacagcc cccagagggg
     5281 ccagacatat ggaggggggc agggagtaag cccagacttc cccgaggcag aggccgctca
     5341 taccccctgt tctgccctag agcctgctgc tgccctggag ccccaggcag ctgctcgggg
     5401 tcccccacca cagtcagtag caggtgggag aagaggaaac tgcttcttca tccaagatga
     5461 tgacagtggg gaggagctca agggtcacgg ggctgcccca cccatacctt caccccctcc
     5521 ctcaccccca ccctcacctg cccccttgga gccgtcaagc agggtaggaa gaggagaggc
     5581 cttgtttgga ggacctgttg gggccagtga actggagccc ttcagtcttt catcattccc
     5641 agaccttatg ggagaactca tcagtgacga agctccaagc atccctgctc cgacccccca
     5701 gctgtctcct gctcttagca ccatcacaga cttctcccca gagtggtcct acccagaggg
     5761 tggggtcaag gtgctcatca caggtccttg gaccgaagcc gccgagcatt actcctgtgt
     5821 ctttgatcac atcgcagtgc cagcctcact tgtccagcct ggtgtcttac gctgctactg
     5881 tcccgcccat gaggtagggc tggtgtcttt gcaggtggca gggcgggagg ggcccctttc
     5941 tgcttctgtg ctctttgagt atcgagcccg ccgattcctg tctctgccta gtactcaact
     6001 tgactggctg tcactggacg acaaccagtt ccggatgtcc atactagagc gactggagca
     6061 gatggagaag cggatggcag agatcgcagc agctgggcag gtgccttgcc agggtcctga
     6121 tgctcctcca gttcaggatg aaggccaggg gcctgggttc gaagcacggg tagtggtctt
     6181 ggtagaaagc atgatcccac gctccacctg gaagggtcct gaacgtctgg cccatggaag
     6241 ccccttccgg ggcatgagcc ttctgcacct ggctgctgcc cagggctatg cccgcctcat
     6301 cgagaccctg agccagtggc ggagtgtgga gactggaagc ttggacttag agcaggaggt
     6361 tgacccgctc aacgtggatc atttctcttg cacccctctg atgtgggctt gtgccctggg
     6421 acacctggaa gctgctgtgc tccttttccg ttggaaccga caggcactga gcattcccga
     6481 ctctctgggc cgtctgccat tgtctgtggc tcattcccgg ggtcatgtgc gccttgcccg
     6541 ctgccttgag gaactacaga gacaggagcc ttcggtggag cccccatttg ccctatcgcc
     6601 accctcctcc agcccagaca ctggtctgag cagcgtctcc tcgccctcgg agctgtcgga
     6661 tggcaccttt tccgtcacgt cagcctattc tagtgcccca gatggcagtc ccccccctgc
     6721 acctctgcca gcctctgaga tgactatgga ggacatggcc ccaggccagc tttcctctgg
     6781 tgtcccagaa gcccccctac tcctcatgga ctatgaggct accaactcca aggggcccct
     6841 ctcctccctt cctgccctcc caccagcttc agatgatggg gctgctccag aggacgctga
     6901 cagcccacag gctgtggatg tgatcccggt ggacatgatc tcactagcca agcagatcat
     6961 cgaagccaca ccggagcgga ttaaacgaga ggacttcgtg gggctgcccg aggctggagc
     7021 ctcaatgcgg gagcggacag gggctgtggg gctcagtgag accatgtcct ggctggccag
     7081 ctacctggag aatgtggacc atttccccag ctcaacccct cccagcgaac tgccctttga
     7141 gcgaggtcgc ctggctgtcc cttcagcacc ctcctgggca gagtttctct ctgcatccac
     7201 cagtggcaag atggaaagtg attttgccct gctgacacta tcagatcacg agcagcggga
     7261 actgtatgag gctgcccgag tcatccagac ggccttccga aagtacaagg gccggcggct
     7321 gaaggagcag caggaggtag cagcagctgt aatccagcgc tgttaccgga agtacaagca
     7381 gtttgcactc tataagaaga tgacccaggc ggccatcctg atccagagca agttccgaag
     7441 ctactatgaa cagaagcgat ttcagcagag ccgccgagcg gctgtgctca tccagcagca
     7501 ctaccgctcc taccgccgca ggcccggccc tccccaccgg acttcggcca ccctgcctgc
     7561 ccgcaacaaa ggctcctttc tcaccaagaa gcaggaccag gcagcccgga agatcatgag
     7621 attcctgcgg cgctgccgac acaggatgag ggaactgaag cagaaccagg agctggaagg
     7681 gcttccccag ccgggactgg ccacatagta atgaactagt ccgtaacttg aaagtatttc
     7741 gatttcttgg ctttatatat cttgtggaaa ggacgatgag caaccggcaa cctattgcat
     7801 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7861 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7921 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7981 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8041 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8101 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8161 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8221 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8281 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8401 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8461 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8521 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8581 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8641 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8701 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8761 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8881 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8941 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9001 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9061 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9121 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9181 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9241 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9301 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9361 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9421 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9481 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9541 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9601 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9661 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9721 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9901 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9961 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10021 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10081 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10141 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10201 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10261 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10321 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10381 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10441 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10501 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10561 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10621 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10681 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11701 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11761 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11821 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11881 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    11941 aaaagctgga gctgcaagc
//
