LOCUS       sequence-395984-       13873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0278
FEATURES             Location/Qualifiers
     source          1..13873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9730..10318
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9783..9803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10201..10212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10390..10623
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10701..10822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10738..10757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10792..10811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10862..10997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11017..11036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11018..11036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11046..11063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11046..11062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11055..11077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11204..11659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11291..11310)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11501..11522
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11685..11789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11790..11858,11859..12650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12008..12027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12821..13409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13310..13329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13563..13580
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13697..13718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13733..13738,13739..13756,13757..13763)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13771..13787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13776..13798
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13795..13811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13795..13811
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13830..13850
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13832..13850
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggca aactcgacgg ggaaggcgcc
     4141 tccggacgag cggagaaagg gactcgcttt cctggacgag ctgcggcagt tccaccacag
     4201 cagagggtcg ccttttaaaa aaatccctgc ggtgggtggg aaggagctgg atcttcacgg
     4261 tctctacacc agagtcacta ctttaggcgg attcgcgaag gtttctgaga agaatcagtg
     4321 gggagaaatt gttgaagagt tcaactttcc cagaagttgt tctaacgctg cctttgcttt
     4381 aaaacagtat tacttgcgtt acctagaaaa gtacgagaaa gttcatcatt ttggggagga
     4441 tgatgatgag gtaccaccag gcaatccaaa gccacagctt cctattggtg caattccatc
     4501 ttcctacaat taccagcaac acagtgtgtc ggattatctg cgtcaaagtt atgggctgtc
     4561 catggacttt aattcgccaa atgattataa taaattggtg ctttcactgt tatctggact
     4621 cccaaatgaa gtggactttg ctattaacgt atgcactctc ctatcaaatg aaagcaagca
     4681 cgtcatgcaa cttgaaaaag atcctaaaat catcacttta ctacttgcta atgccggggt
     4741 gtttgacgac actttaggat ccttttccac tgtatttgga gaagaatgga aagagaagac
     4801 tgatagagac ttcgttaagt tttggaaaga catcgttgat gataatgaag ttcgtgacct
     4861 catttctgac agaaacaagt ctcatgaagg tacatcagga gaatggattt gggagtcttt
     4921 atttcatcca cctcgaaagc tgggcattaa cgatattgaa ggacagcggg tacttcagat
     4981 tgcagtgatt ttgagaaatc tttcctttga ggagggcaat gttaagctct tggcagctaa
     5041 tcgtacctgt cttcgtttcc tattactttc tgcacatagt cattttattt ctttaaggca
     5101 attaggcctt gacacattag gaaatattgc agctgagctt ttactggacc ctgttgattt
     5161 caaaactact catctgatgt ttcatactgt tacaaaatgt ctaatgtcaa gggatagatt
     5221 tttaaagatg agaggcatgg aaattttggg aaatctttgc aaagcagaag ataatggtgt
     5281 tttaatttgt gaatatgtgg atcaggattc ctacagagag atcatttgtc atctcacttt
     5341 acctgatgtg ctgcttgtaa tctcaacact cgaggtgcta tacatgctca cggaaatggg
     5401 agatgttgct tgcacaaaaa ttgcaaaagt agaaaagagc atagacatgt tagtgtgtct
     5461 ggtttctatg gatattcaga tgtttggccc tgatgcacta gctgcggtaa aactcattga
     5521 acacccaagt tccagtcatc aaatgttatc tgaaattagg ccacaagcta tagagcaagt
     5581 ccaaacccag actcatgtag catctgcccc agcttccaga gcagttgtag cgcagcatgt
     5641 tgctccacct ccaggaatag tggaaataga tagtgagaag tttgcttgtc agtggctaaa
     5701 tgctcatttt gaagtaaatc cagattgttc tgtttctcga gcagaaatgt attctgaata
     5761 cctctcgact tgcagtaaat tagctcgtgg tggaatccta acatcaactg gattttataa
     5821 atgtcttaga acggtctttc caaatcatac agtgaagaga gtggaggatt ccagtagcaa
     5881 tgggcaggca catattcatg tggtaggagt aaaacggagg gctataccac ttcccattca
     5941 gatgtactat cagcagcaac cagtttctac ttctgttgtt cgtgttgatt ctgttcctga
     6001 tgtatctcct gctccttcac ctgcaggaat ccctcatgga tcacaaacca taggaaacca
     6061 ttttcagagg actcctgttg ccaaccaatc ttcaaatctg actgcaacac aaatgtcttt
     6121 tcctgtacaa ggtgttcata ctgtggcaca aactgtttca agaattccac aaaatccttc
     6181 acctcatacc caccagcaac aaaatgctcc agtgactgtc attcaaagta aagctccaat
     6241 tccttgtgaa gttgttaagg ctacagttat ccagaattcc ataccccaga caggagttcc
     6301 tgttagtatt gctgttggag gaggacctcc acagagttct gttgttcaga atcatagtac
     6361 agggccacaa cctgttacag ttgtgaattc tcagacattg cttcaccatc catctgtaat
     6421 tccacagcag tctccattac acacagtggt accaggacag atcccttcag gcactcctgt
     6481 tacagtaatt caacaagctg tcccacagag tcatatgttt ggcagagtac agaacatacc
     6541 agcatgtact tctacagttt cacagggtca acagttaatc accacatcac cccaacctgt
     6601 gcaaacttca tctcaacaga catcagctgg tagccagtca caagatactg ttatcatagc
     6661 acccccacag tatgtaacaa cttctgcatc caatattgtc tcagcaactt cagtacagaa
     6721 ttttcaggta gctacaggac aaatggttac tattgctggt gtcccaagtc cacaagcctc
     6781 aagggtaggg tttcagaaca ttgcaccaaa acctctccct tctcagcaag tttcatctac
     6841 agtggtacag cagcctattc aacaaccaca gcagccaacc caacaaagcg tagtgattgt
     6901 aagccagcca gctcaacaag gtcaaactta tgcaccagcc attcaccaaa ttgttcttgc
     6961 taatccagca gctcttccag ctggtcagac agttcagcta actggacaac ctaacataac
     7021 tccatcttct tcaccatcac ctgtcccagc tactaataac caagtcccta ctgccatgtc
     7081 gtcgtcctct acccctcaat cacagggacc acctcctact gtcagtcaaa tgttatctgt
     7141 gaaaaggcag caacagcagc aacattcacc agcaccccca ccacagcagg tacaagtaca
     7201 agttcagcag ccccaacaag tacagatgca agttcaacct caacagtcga atgcaggagt
     7261 tggtcagcct gcctctggtg agtcgagtct gattaaacag cttctgcttc cgaaacgtgg
     7321 tccttcaaca ccaggtggta agcttattct cccagctcca cagattcctc cccctaataa
     7381 tgcaagagct cctagccctc aggtggtcta tcaggtggcc agtaaccaag ccgcaggttt
     7441 tggagtgcag gggcaaactc cagctcagca gctattggtt gggcagcaaa atgttcagtt
     7501 ggtcccaagt gcaatgccac cctcaggggg agtacaaact gtgcccattt cgaacttaca
     7561 aatattgcca ggtccactga tctcaaatag cccagcaacc attttccaag ggacttctgg
     7621 caaccaggta accataacag ttgtgccaaa tacgagtttt gcacctgcaa ctgtgagtca
     7681 gggaaatgca actcagctca ttgctccagc aggaattacc atgagcggaa cgcagacagg
     7741 agttggactt ccagtacaaa cgcttccagc cactcaagca tctcctgctg gacaatcatc
     7801 atgtactact gctactcccc cattcaaagg tgataaaata atttgccaaa aggaggagga
     7861 agcaaaggaa gcaacaggtt tacatgttca tgaacgtaaa attgaagtca tggagaaccc
     7921 gtcctgccga cgaggagcca caaacaccag caatggggat acaaaggaaa atgaaatgca
     7981 tgtgggaagt cttttaaatg ggagaaagta cagtgactca agtctacctc cttcaaactc
     8041 agggaaaatt caaagtgaga ctaatcagtg ctcactaatc agtaatgggc catcattgga
     8101 attaggtgag aatggagcat ctgggaaaca gaactcagaa caaatagaca tgcaagatat
     8161 caaaagtgat ttgagaaaac cgctagttaa tggaatctgt gattttgata aaggagatgg
     8221 ttctcattta agcaaaaaca ttccaaatca taaaacttcc aatcatgtag gaaatggtga
     8281 gatatctcca atggaaccac aagggacttt agatatcact cagcaagata ctgccaaagg
     8341 tgatcaacta gaaagaattt ctaatggacc tgtattaact ttgggtggtt catctgtgag
     8401 cagtatacag gaggcttcaa atgcggcaac acagcaattt agtggtactg atttgcttaa
     8461 tggacctcta gcttcaagtt tgaattcaga tgtgcctcag caacgcccaa gtgtagttgt
     8521 ctcaccacat tctacaacct ctgttataca gggacatcaa atcatagcag ttcccgactc
     8581 aggatcaaaa gtatcccatt ctcctgccct atcatctgac gttcggtcta caaatggcac
     8641 agcagaatgc aaaactgtaa agaggccagc agaggatact gatagggaaa cagtcgcagg
     8701 aattccaaat aaagtaggag ttagaattgt tacaatcagt gaccccaaca atgctggctg
     8761 cagcgcaaca atggttgctg tgccagcagg agcagatcca agcactgtag ctaaagtagc
     8821 aatagaaagt gctgttcagc aaaagcaaca gcatccacca acatatgtac agaatgtggt
     8881 cccgcagaac actcctatgc caccttcacc agctgtacaa gtgcagggcc agcctaacag
     8941 ttctcagcct tctccattca gtggatccag tcagcctgga gatccaatga gaaaacctgg
     9001 acagaacttc atgtgtctgt ggcagtcttg taaaaagtgg tttcagacac cctcacaggt
     9061 tttctaccat gcagcaactg aacatggagg aaaagatgta tatccagggc agtgtctttg
     9121 ggaaggttgt gagccttttc agcgacagcg gttttctttt attacccact tgcaggataa
     9181 gcactgttca aaggatgccc tacttgcagg attaaaacaa gatgaaccag gacaagcagg
     9241 aagtcagaag tcttctacca agcagccaac tgtagggggc acaagctcaa ctcctagagc
     9301 acaaaaggcc attgtgaatc atcccagtgc tgcacttatg gctctgagga gaggatcaag
     9361 aaaccttgtc tttcgagatt ttacagatga aaaagaggga ccaataacta aacacatccg
     9421 actaacagct gccttaatat taaaaaatat tggtaaatat tcagaatgtg gtcgcagatt
     9481 gttaaagaga catgaaaata acttatcagt gctagccatt agtaacatgg aagcttcctc
     9541 cacccttgcc aaatgccttt atgaacttaa ttttacagtt cagagtaagg aacaagaaaa
     9601 agactcagaa atgctgcagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     9661 ttggctttat atatcttgtg gaaaggacga cttaagtcca ttaaatatgg gtggacgcgt
     9721 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     9781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10201 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10261 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    10321 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10381 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10441 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10501 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10561 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10621 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10681 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    10741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10801 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    10861 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10921 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10981 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    11041 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11101 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11161 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    11221 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11281 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    11341 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    11401 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    11461 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    11521 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    11581 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    11641 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    11701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    11761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    11821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    11881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    11941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    12001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    12061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    12121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    12181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    12241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    12301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    12361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    12421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    12481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    12541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    12601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    12661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    12721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    12781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    12841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    12901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    12961 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    13021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    13081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    13141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    13201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    13261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    13321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    13381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    13441 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    13501 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    13561 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    13621 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    13681 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    13741 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    13801 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    13861 tggagctgca agc
//
