LOCUS       sequence-39599-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1420
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accatggtgg cggaagttcc tatacctttt gaagaatagg aacttcggcg cgccacgcgt
       61 ctgctgcagc ctcctccaga cgcgccgccg cctccggttg ctgcacccag ctcggagccc
      121 gcagttttca ccggggatgg aggagagggg gagcggggag ccgggggctc agccaatcag
      181 aggcggagat tgggggagaa aggacggcag ggtggggtgg ggggtgggga aggaagagaa
      241 ggcggtcccg cattggtgtg agagtggcag gttgcggcgg aagggggccc tcaccctgtg
      301 gctggcaggc ctggccaggg acgggagggt tcgggacccc ggggatcagg gtgcggcagc
      361 ccaaagaaca ttatcctcct ccctgagccc cgcaatggtt gttccccttt taaggcttta
      421 accctttctt ctgacctatt cttcaggagt ctgtgggaaa accaaagtgt aagaggtgca
      481 ggatgcaggc cgggagcgcc cacgcctcca catttccttt tcagctggag cccacctcct
      541 ccggagcctc cagtgagaag ttttggaata ttcca
//
