LOCUS       sequence-396060-       10633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1869
FEATURES             Location/Qualifiers
     source          1..10633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6490..7078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6543..6563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6961..6972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7150..7383
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7461..7582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7498..7517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7552..7571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7622..7757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7777..7796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7778..7796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7806..7823)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7806..7822)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7815..7837)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7964..8419
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8051..8070)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8261..8282
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8445..8549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8550..8618,8619..9410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8768..8787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9581..10169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10070..10089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10323..10340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10457..10478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10493..10498,10499..10516,10517..10523)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10531..10547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10536..10558
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10555..10571
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10555..10571
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10590..10610
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10592..10610
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgctg gactgcagtg actatgttct
     4141 agactcaaga atgaacaatc cgtcagaaac cagtaaacca tctatggaga gtggagatgg
     4201 caacacaggc acacaaacca atggtctgga ctttcagaag cagcctgtgc ctgtaggagg
     4261 agcaatctca acagcccagg cgcaggcttt ccttggacat ctccatcagg tccaactcgc
     4321 tggaacaagt ttacaggctg ctgctcagtc tttaaatgta cagtctaaat ctaatgaaga
     4381 atcgggggat tcgcagcagc caagccagcc ttcccagcag ccttcagtgc aggcagccat
     4441 tccccagacc cagcttatgc tagctggagg acagataact gggcttactt tgacgcctgc
     4501 ccagcaacag ttactactcc agcaggcaca ggcacaggca cagctgctgg ctgctgcagt
     4561 gcagcagcac tccgccagcc agcagcacag tgctgctgga gccaccatct ccgcctctgc
     4621 tgccacgccc atgacgcaga tccccctgtc tcagcccata cagatcgcac aggatcttca
     4681 acaactgcaa cagcttcaac agcagaatct caacctgcaa cagtttgtgt tggtgcatcc
     4741 aaccaccaat ttgcagccag cgcagtttat catctcacag acgccccagg gccagcaggg
     4801 tctcctgcaa gcgcaaaatc ttctaacgca actacctcag caaagccaag ccaacctcct
     4861 acagtcgcag ccaagcatca ccctcacctc ccagccagca accccaacac gcacaatagc
     4921 agcaacccca attcagacac ttccacagag ccagtcaaca ccaaagcgaa ttgatactcc
     4981 cagcttggag gagcccagtg accttgagga gcttgagcag tttgccaaga ccttcaaaca
     5041 aagacgaatc aaacttggat tcactcaggg tgatgttggg ctcgctatgg ggaaactata
     5101 tggaaatgac ttcagccaaa ctaccatctc tcgatttgaa gccttgaacc tcagctttaa
     5161 gaacatgtgc aagttgaagc cacttttaga gaagtggcta aatgatgcag agaacctctc
     5221 atctgattcg tccctctcca gcccaagtgc cctgaattct ccaggaattg agggcttgag
     5281 ccgtaggagg aagaaacgca ccagcataga gaccaacatc cgtgtggcct tagagaagag
     5341 tttcttggag aatcaaaagc ctacctcgga agagatcact atgattgctg atcagctcaa
     5401 tatggaaaaa gaggtgattc gtgtttggtt ctgtaaccgc cgccagaaag aaaaaagaat
     5461 caacccacca agcagtggtg ggaccagcag ctcacctatt aaagcaattt tccccagccc
     5521 aacttcactg gtggcgacca caccaagcct tgtgactagc agtgcagcaa ctaccctcac
     5581 agtcagccct gtcctccctc tgaccagtgc tgctgtgacg aatctttcag ttacaggcac
     5641 ttcagacacc acctccaaca acacagcaac cgtgatttcc acagcgcctc cagcttcctc
     5701 agcagtcacg tccccctctc tgagtccctc cccttctgcc tcagcctcca cctccgaggc
     5761 atccagtgcc agtgagacca gcacaacaca gaccacctcc actcctttgt cctcccctct
     5821 tgggaccagc caggtgatgg tgacagcatc aggtttgcaa acagcagcag ctgctgccct
     5881 tcaaggagct gcacagttgc cagcaaatgc cagtcttgct gccatggcag ctgctgcagg
     5941 actaaaccca agcctgatgg caccctcaca gtttgcggct ggaggtgcct tactcagtct
     6001 gaatccaggg accctgagcg gtgctctcag cccagctcta atgagcaaca gtacactggc
     6061 aactattcaa gctcttgctt ctggtggctc tcttccaata acatcacttg atgcaactgg
     6121 gaacctggta tttgccaatg cgggaggagc ccccaacatc gtgactgccc ctctgttcct
     6181 gaaccctcag aacctctctc tgctcaccag caaccctgtt agcttggtct ctgccgccgc
     6241 agcatctgca gggaactctg cacctgtagc cagccttcac gccacctcca cctctgctga
     6301 gtccatccag aactctctct tcacagtggc ctctgccagc ggggctgcgt ccaccaccac
     6361 caccgcctcc aaggcacagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     6421 ttggctttat atatcttgtg gaaaggacga aacgctccag catgcccgct ggaaacgcgt
     6481 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6541 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6601 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6661 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6721 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6781 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6841 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6901 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6961 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7021 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     7081 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7141 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7201 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7261 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7321 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7381 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7441 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7561 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7681 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7741 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7801 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7861 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7921 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7981 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8041 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8101 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8161 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8221 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8281 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8341 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8401 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8461 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8521 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8581 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8641 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8701 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8761 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8821 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8881 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8941 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9001 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9061 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9121 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9181 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9241 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9301 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9361 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9541 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9601 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9661 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9721 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9781 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9841 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9901 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9961 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10021 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10081 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10141 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10201 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10261 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10321 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10381 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    10441 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10501 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10561 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10621 tggagctgca agc
//
