LOCUS       sequence-396067-       10774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2022
FEATURES             Location/Qualifiers
     source          1..10774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6631..7219
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6684..6704)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7102..7113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7291..7524
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7602..7723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7639..7658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7693..7712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7763..7898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7918..7937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7919..7937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7947..7964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7947..7963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7956..7978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8105..8560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8192..8211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8402..8423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8586..8690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8691..8759,8760..9551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8909..8928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9722..10310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10211..10230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10464..10481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10598..10619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10634..10639,10640..10657,10658..10664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10672..10688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10677..10699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10696..10712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10696..10712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10731..10751
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10733..10751
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtac cgctccacca agggcgcctc
     4141 caaggcgcgc cgggaccaga tcaacgccga gatccggaac ctcaaggagc tgctgccgct
     4201 ggccgaagcg gacaaggtcc ggctgtccta cctgcacatc atgagcctcg cctgcatcta
     4261 cactcgcaag ggcgtcttct tcgctggtgg cactcctctg gcgggcccca cggggcttct
     4321 ctcagctcaa gagcttgagg acatcgtagc ggcactaccc ggctttctgc ttgtgttcac
     4381 agccgagggg aaattgctct acctgtctga gagtgtgagc gagcatctgg gccactccat
     4441 ggtggacctg gttgcccagg gtgacagcat ctacgacatc attgacccag ctgaccacct
     4501 cactgtgcgc cagcaactca ccctgccctc tgccctggac actgatcgcc tcttccgctg
     4561 ccgcttcaac acctccaagt ccctcaggcg ccagagtgca ggcaacaaac tcgtgcttat
     4621 tcgaggccga ttccatgctc acccacctgg agcctactgg gcaggaaatc ccgtgttcac
     4681 agctttctgt gcccctctgg agccgagacc ccgcccaggt cctggccctg gccctggccc
     4741 tgcctcgctc ttcctggcca tgttccagag ccgccatgct aaagacctgg ctctactgga
     4801 catctccgag agtgtcctaa tctacctggg ctttgagcgc agtgaactgc tttgtaaatc
     4861 atggtatgga ctgctgcacc ccgaggacct ggcccacgct tctgctcaac actaccgcct
     4921 gttggctgag agtggagata ttcaggcaga gatggtggtg aggctacagg ccaagactgg
     4981 aggctgggca tggatttact gcctgttata ctcagaaggt ccagagggac ccattactgc
     5041 caataactac ccaatcagtg acatggaagc ctggagcctc cgccagcagt tgaactctga
     5101 agacacccag gcagcttatg tcctgggcac tccgaccatg ctgccctcat tccctgaaaa
     5161 cattctttcc caggaagagt gctccagcac taacccactc ttcaccgcag cactgggggc
     5221 tcccagaagc accagcttcc ccagtgctcc tgaactgagt gttgtctctg catcagaaga
     5281 gcttccccga ccctccaaag aactggactt cagttacctg acattccctt ctgggcctga
     5341 gccttctctc caagcagaac taagcaagga tcttgtgtgc actccacctt acacgcccca
     5401 tcagccagga ggctgtgcct tcctcttcag cctccatgag cccttccaga cccatttgcc
     5461 caccccatcc agcactcttc aagaacagct gactccaagc actgcgacct tctctgatca
     5521 gttgacgccc agcagtgcaa ccttcccaga tccactaact agcccactgc aaggccagtt
     5581 gactgaaacc tcggtcagaa gctatgaaga ccagttgact ccctgcacct ccaccttccc
     5641 agaccagctg cttcccagca cagccacctt cccagagcct ctgggcagcc ctgcccatga
     5701 acagctgact cctcccagca cagcattcca agcacacctg gacagcccca gccaaacctt
     5761 cccagagcaa ctgagcccca accctaccaa gacttacttt gcccaggagg gatgcagttt
     5821 tctctatgag aagttgcccc caagtcctag cagccctggt aatggggact gcacgctctt
     5881 ggccctagcc cagctccggg gccccctctc tgtggatgtc cccctggtgc ccgaaggcct
     5941 gctcacacct gaggcctctc cagtcaagca gagtttcttc cactactctg aaaaggagca
     6001 gaatgagata gaccgtctca tccagcagat tagccaattg gctcagggca tggacagacc
     6061 cttctcagct gaggctggca ctggcggact agagccactt ggaggactgg agcccctgga
     6121 ctccaacctg tccctgtcag gggcaggccc ccctgtgctc agcctggacc tgaaaccctg
     6181 gaaatgccag gagctggact tcctggctga ccctgataac atgttcctgg aagagacgcc
     6241 cgtggaagac atcttcatgg atctctctac cccagatccc agtgaggaat ggggctcagg
     6301 ggatcctgag gcagagggcc caggaggggc cccatcgcct tgcaacaacc tgtccccaga
     6361 agaccacagc ttcctggagg acctggccac atatgaaacc gcctttgaga caggtgtctc
     6421 agcattcccc tatgatgggt ttactgatga gttgcatcaa ctccagagcc aagttcaaga
     6481 cagcttccat gaagatggaa gtggagggga accaacgttt tagtaatgaa ctagtccgta
     6541 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaaactcgcg
     6601 tcgcgtaaat tcgggacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6661 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6721 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6781 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6841 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6901 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6961 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7021 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7081 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7141 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7201 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     7261 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7321 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7381 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7441 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7501 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7561 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7621 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7681 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7741 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7861 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7921 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10681 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10741 ctcactaaag ggaacaaaag ctggagctgc aagc
//
