LOCUS       sequence-396077-       12244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2014
FEATURES             Location/Qualifiers
     source          1..12244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8101..8689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8154..8174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8572..8583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8761..8994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9072..9193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9109..9128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9163..9182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9233..9368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9388..9407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9389..9407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9417..9434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9417..9433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9426..9448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9575..10030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9662..9681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9872..9893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10056..10160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10161..10229,10230..11021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10379..10398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11192..11780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11681..11700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11934..11951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12068..12089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12104..12109,12110..12127,12128..12134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12142..12158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12147..12169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12166..12182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12166..12182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12201..12221
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12203..12221
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac gcatcatatg atggtactga
     4141 ggtaactgtc gtgatggagg aaattgagga agcctattgt tacacctctc ctgggccacc
     4201 caagaagaag aaaaagtata aaatacatgg agaaaagaca aagaaaccca ggtctgctta
     4261 ccttctgtac tattacgaca tctacctgaa agtgcagcag gagctccccc acctccctca
     4321 gtctgagatc aataagaaga ttagtgagag ttggaggctt ctcagcgtgg ccgagaggag
     4381 ttactacttg gagaaagcca aactagagaa ggaaggtttg gatcctaact ctaagctctc
     4441 tgcactgact gctgtggttc cggacatccc aggtttccgc aagatcctcc cacgctcaga
     4501 ttatatcatc atccccaaga gcagcctgca ggaggaccgg agctgccctc agctagagct
     4561 atgtgtggct cagaaccaga tgtccccgaa aggacctcct cttgtgtcca acactgcccc
     4621 ggagacagtg cccagccatg caggcatggc agagcagtgc ctggctgtgg aggccctggc
     4681 tgaggaggtg ggagccctta cccagtcagg tgctgtacag gagattgcca cctcagagat
     4741 cctcagccag gatgtgctcc tagaggacgc ttccctagaa gtaggggaga gccaccaacc
     4801 ttaccagaca agcctggtaa ttgaagagac cttggtgaat ggctcaccag acctccccac
     4861 tggaagcctg gctgtgcccc acccccaggt tggggagagt gtatcagtgg taacagtcat
     4921 gagggattcc agtgagagta gctcctctgc accagccaca cagttcatca tgttgcctct
     4981 gcctgcctac tcggttgtgg agaaccccac ctccatcaaa ctgaccacta catatacccg
     5041 ccggggccat gggacatgca ccagcccagg gtgctccttt acatatgtca ccaggcacaa
     5101 gccacctaag tgccctacct gtggtaactt cctaggaggg aagtggatcc caaaggaaaa
     5161 gccagccaaa gtaaaagtgg aattggcttc tggcgtctct tccaaaggct ctgtggtgaa
     5221 aagaaatcag caacctgtca ccactgagca aaattcctct aaggaaaatg cctccaaact
     5281 gactctggag aattcggaag ctgtaagcca gctcctgaac gtagctcctc ccagagaagt
     5341 aggtgaggag agtgagtggg aggaagtgat catctccgat gcccatgttt tggttaagga
     5401 agctcccggg aattgtggta cagcagtcac taagacgcca gtcgtcaaaa gtggtgtgca
     5461 gcctgaggtc actctgggga caactgacaa tgacagtcct ggagcagacg taccaacacc
     5521 atccgagggg acaagtacct ccagtccact ccctgctcct aaaaaaccta caggagctga
     5581 cctgcttacc cctgggtcca gagctccaga gcttaaaggc agagcacggg gcaagccctc
     5641 attactggct gcagcaagac ccatgagagc aattttgcca gccccagtta acgtggggcg
     5701 aggcagcagc atgggactgc ccagggccag gcaggccttt tccctgagtg ataagactcc
     5761 ctctgtgagg acttgtggtc tgaagccaag cacactgaag cagctgggcc agcccattca
     5821 acagccatct ggccctggtg aggtgaagct accaagtggc ccatccaaca ggacttctca
     5881 ggtgaaagtt gtggaggtca agcccgatat gtttcctcca tataagtaca gctgcactgt
     5941 cacattggat ttgggcctgg ctacatcaag aggccgggga aagtgcaaga atccctcttg
     6001 tagctatgtc tacaccaaca ggcacaaacc tcgaatttgt cccagctgtg gtgttaacct
     6061 tgccaaagac cggactgaga aaaccaccaa ggctatcgag gtgagctcac cactcccaga
     6121 tgtactgaat gccacagagc ccctgagcac agcccagagg gagatccagc gccagtccac
     6181 actgcagctg ctgcgcaaag tcctgcagat tcctgagaat gagtcagagc tggctgaggt
     6241 cttcgccttg attcatgaac tcaacagctc tcgacttatc ttgtccaacg tgagtgagga
     6301 gacagtcacc atcgagcaaa cctcttggtc gaattattat gagtctccgt ccacgcagtg
     6361 ccttctctgt agcagcccat tattcaaagg gggacaaaac tccctggctg ggccccagga
     6421 gtgctggctg ctgacagcca gccgtctgca gacagtgact gcccaggtga agatgtgtct
     6481 gaacccccat tgtctggccc tgcacagctt catagacatc tacacaggtc tctttaatgt
     6541 ggggaacaag ctgctggtaa gcctggactt gctttttgca atcagaaatc agatcaagct
     6601 cggagaggac cccagagtgt ccatcaatgt tgttctgaag tcggtgcagg agcagacaga
     6661 gaagactctg acctcggagg agctgagcca gctgcaggag ctgctgtgca atggctattg
     6721 ggcctttgag tgcctcactg tccgagacta caatgacatg atctgtggca tctgtggtgt
     6781 ggcccccaaa gtggaaatgg ctcagaggag tgaagagaat gtgctagcac tgaagagcgt
     6841 ggagttcacc tggcctgaat tcctgggctc taatgaggta aatgtggagg acttttgggc
     6901 cacgatggag acagaggtga ttgagcaggt ggcatttcct gccagcatcc ctatcaccaa
     6961 atttgatgcg tctgttattg cccccttctt cccaccactc atgagaggag ctgtggtcgt
     7021 caacactgag aaagacaaaa acctggatgt gcagccagta cctggcagtg gcagtgcctt
     7081 ggtgaggctg ctccaggagg gcacctgcaa gcttgatgag attggctcct acagtgaaga
     7141 gaagctgcag cacctgctaa ggcagtgtgg aatccccttt ggggcagaag actccaagga
     7201 ccagctctgc ttctccttgt tggccctcta cgaatctgta cagaatggag ctagagctat
     7261 acggccccca cgtcacttca caggtggtaa aatctacaag gtgtgccccc atcaggtggt
     7321 ctgcggctcc aagtatcttg tgcgaggtga gagtgcccgt gaccatgtgg acctgcttgc
     7381 ctcttcccgc cactggccgc ctgtctatgt ggtagatatg gccacgtcag tggccctgtg
     7441 tgctgacctc tgctacccag agctgactaa ccagatgtgg gggaggaacc agggctgttt
     7501 ctctagcccc acagagccac ctgtgagtgt gtcctgccca gagctcttgg accagcatta
     7561 tactgtggac atgacagaaa ctgagcactc tatccagcac ccagtcacca agactgccac
     7621 gcggcgcatc gtccatgcag gcctacagcc caatcctggt gaccccagtg ctgggcacca
     7681 ctccttggcc ctgtgccctg aattggcacc ttacgcaacc atcctggcct ccatcgtgga
     7741 cagcaaacca aacggtgtcc gccagcggcc cattgccttc gacaatgcca ctcactatta
     7801 cctctacaac cgcctcatgg acttcctcac cagccgcgaa attgtcaatc gtcagatcca
     7861 tgacattgta cagagctgcc agcctggtga ggtggtcatt cgtgacaccc tctaccgcct
     7921 tggggttgct cagatcaaga cagagacaga ggaggagggt gaggaagagg aggtggccgc
     7981 agtggcagaa tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     8041 tatatcttgt ggaaaggacg aaagtgtgtg ggtgccatgg tatacacgcg ttaagtcgac
     8101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8221 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8281 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8341 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8401 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8461 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8521 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8581 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8641 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     8701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8761 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     9001 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9061 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9181 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     9241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9361 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9541 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    10021 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    11041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    12001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    12061 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    12121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    12181 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    12241 aagc
//
