LOCUS       sequence-396101-       14230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0659
FEATURES             Location/Qualifiers
     source          1..14230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10087..10675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10140..10160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10558..10569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10747..10980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11058..11179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11095..11114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11149..11168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11219..11354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11374..11393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11375..11393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11403..11420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11403..11419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11412..11434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11561..12016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11648..11667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11858..11879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12042..12146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12147..12215,12216..13007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12365..12384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13178..13766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13667..13686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13920..13937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14054..14075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14090..14095,14096..14113,14114..14120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14128..14144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14133..14155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14152..14168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14152..14168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14187..14207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14189..14207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcgg ggcccagtgg gcaccgagga
     4141 ggagctgccg cggctgttcg ccgaggagat ggagaatgag gacgagatgt cagaagaaga
     4201 agatggtggt cttgaagcct tcgatgactt tttccctgtg gagcccgtga gccttcctaa
     4261 gaagaagaaa cccaagaagc tcaaggaaaa caagtgtaaa gggaagcgga agaagaaaga
     4321 ggggagcaat gatgagctat cagagaatga agaggatctg gaagagaagt cggagagtga
     4381 aggcagtgac tactccccga ataaaaagaa gaagaagaaa ctcaaggaca agaaggagaa
     4441 aaaagccaag cgaaaaaaga aggatgagga tgaggatgat aatgatgatg gatgcttaaa
     4501 ggagcccaag tcctcggggc agctcatggc cgagtggggc ctggacgacg tggactacct
     4561 gttctcggag gaggattacc acacgctgac caactacaag gccttcagcc agttcctcag
     4621 gccactcatt gccaagaaga acccgaagat ccccatgtcc aaaatgatga ccgtcctggg
     4681 tgccaagtgg cgggagttca gcgccaacaa ccccttcaag ggcagctccg cggcagcagc
     4741 ggcggcggcg gtggctgcgg ctgtagagac ggtcaccatc tcccctccgc tagccgtcag
     4801 ccccccgcag gtgccccagc ctgtgcctat ccgcaaggcc aagaccaagg agggcaaagg
     4861 gcctggagtg aggaagaaga tcaaaggctc caaagatggg aagaaaaagg gcaaagggaa
     4921 aaagacggcc gggctcaagt tccgcttcgg ggggatcagc aacaagagga agaaaggctc
     4981 ctcgagtgaa gaagatgaga gggaggagtc ggacttcgac agcgccagca tccacagtgc
     5041 ctccgtgcgc tccgaatgct ctgcagccct gggcaagaag agcaagagga ggcgcaagaa
     5101 gaagaggatt gatgatggtg acggctatga gacagaccac caggattact gtgaggtgtg
     5161 ccagcagggt ggggagatca tcctgtgcga cacctgcccg agggcctacc atctcgtatg
     5221 cctggaccca gagctggaga aggctcccga gggcaagtgg agctgccccc actgtgagaa
     5281 ggaggggatc cagtgggagc cgaaggacga cgacgatgaa gaggaggagg gcggctgcga
     5341 ggaggaggag gacgaccaca tggagttctg ccgcgtgtgc aaggacgggg gcgagctgct
     5401 ctgctgcgac gcctgcccct cctcctacca cctgcattgc ctcaacccgc cgctgcccga
     5461 gatcccaaac ggtgaatggc tctgcccgcg ctgtacttgc cccccactga agggcaaagt
     5521 ccagcggatt ctacactgga ggtggacgga gccccctgcc cccttcatgg tggggctgcc
     5581 ggggcctgac gtggagccca gcctccctcc acctaagccc ctggagggca tccctgagag
     5641 agagttcttt gtcaagtggg cagggctgtc ctactggcat tgctcctggg tgaaggagct
     5701 acagctggag ctgtaccaca cggtgatgta tcgcaactac caaagaaaga acgacatgga
     5761 tgagccgccc ccctttgact acggctctgg ggatgaagac ggcaagagcg agaagaggaa
     5821 gaacaaggac cccctctatg ccaagatgga ggagcgcttc taccgctatg gcatcaagcc
     5881 agagtggatg atgattcacc gaatcctgaa ccatagcttt gacaagaagg gggatgtgca
     5941 ctacctgatc aagtggaaag acctgcccta cgaccagtgc acctgggaga tcgatgacat
     6001 cgacatcccc tactacgaca acctcaagca ggcctactgg ggccacaggg agctgatgct
     6061 gggagaagac accaggctgc ccaagaggct gctcaagaag ggcaagaagc tgagggacga
     6121 caagcaggag aagccgccgg acacgcccat tgtggacccc acggtcaagt tcgacaagca
     6181 gccatggtac atcgactcca caggcggcac actgcacccg taccagctgg agggcctcaa
     6241 ctggctgcgc ttctcttggg cccagggcac tgacaccatc ctggccgatg agatgggtct
     6301 gggcaagacg gtgcagacca tcgtgttcct ttactccctc tacaaggagg gccactccaa
     6361 agggccctac ctggttagcg cgcccctctc caccatcatc aactgggaac gcgagtttga
     6421 gatgtgggcg cccgacttct acgtggtcac ctacacgggg gacaaggaga gccgctcggt
     6481 gattcgggag aacgagtttt cctttgagga caacgccatt cggagtggga agaaggtatt
     6541 ccgtatgaag aaagaagtgc agatcaaatt ccacgtgctg ctcacctcct atgagctcat
     6601 caccattgac caggccatcc tgggctccat cgagtgggcc tgcctggtgg tagatgaggc
     6661 ccaccgcctc aagaacaacc agtccaagtt ttttagggtc ttaaacagct acaagattga
     6721 ttacaagctg ctgctgacag ggacccccct tcagaacaac ctggaggagc tgttccatct
     6781 cctcaacttc ctgactccag agaggttcaa caacctggag ggcttcctgg aggagtttgc
     6841 tgacatctcc aaggaagacc agatcaagaa gctgcatgac ctgctggggc cgcacatgct
     6901 caggcggctc aaggctgacg tgttcaagaa catgccggcc aagaccgagc tcattgtccg
     6961 ggtggagctg agccagatgc agaagaagta ctacaagttc atcctcacac ggaactttga
     7021 ggcactgaac tccaaggggg gcgggaacca agtatcgctg ctcaacatca tgatggacct
     7081 gaaaaagtgc tgcaaccacc cctacctctt ccctgtggct gccgtggagg cccctgtctt
     7141 gcccaatggc tcctacgatg gaagctccct ggtcaagtct tcagggaagc tcatgctgct
     7201 acagaagatg ctgaagaaac tgcgggatga ggggcaccgt gtgctcatct tctcccagat
     7261 gaccaagatg ctggacctcc tggaggactt cctggagtac gaaggctaca agtatgagcg
     7321 gattgatggt ggcatcaccg ggggcctccg gcaggaggca atcgacagat tcaatgcccc
     7381 cggggcccag cagttctgct tcctcctctc aacccgggca ggtggtctgg gcatcaacct
     7441 ggccacggcg gacactgtca tcatctacga ctcggactgg aacccgcaca atgacatcca
     7501 ggccttcagc cgcgcccacc gcatcggcca gaacaagaag gtgatgatct accgcttcgt
     7561 gactcgggcc tcggtggagg agcgcatcac gcaggtggcc aagcgcaaga tgatgctcac
     7621 ccacctggtg gtgcggcccg gcctcggctc caagtcgggg tccatgacca agcaggagct
     7681 ggacgacatc ctcaagttcg gcacggagga actcttcaag gacgacgtgg agggcatgat
     7741 gtctcagggc cagaggccgg tcacacccat ccctgatgtc cagtcctcca aaggggggaa
     7801 cttggccgcc agtgcaaaga agaagcacgg tagcaccccg ccaggtgaca acaaggacgt
     7861 ggaggacagc agtgtgatcc actatgacga tgcggccatc tccaagctgc tggaccggaa
     7921 ccaggacgct acagatgaca cggagctaca gaacatgaac gagtacctga gctccttcaa
     7981 ggtggcgcag tacgtggtgc gcgaggagga cggcgtggag gaggtggagc gggaaatcat
     8041 caagcaggag gagaacgtgg accccgacta ctgggagaag ctgctgcggc accactatga
     8101 gcagcagcag gaggacctgg cccgcaacct gggcaagggc aagcgcatcc gcaagcaggt
     8161 caactacaac gatgcctccc aggaggacca ggagtggcag gatgagctct ctgataacca
     8221 gtcagaatat tccattggct ctgaggatga ggatgaggac tttgaagaga ggccggaagg
     8281 gcagagtgga cgacgacaat cccggaggca gctgaagagt gacagggaca agcccctgcc
     8341 cccgcttctc gcccgagttg gtggcaacat cgaggtgctg ggcttcaatg cccgacagcg
     8401 gaaggccttt ctgaacgcca tcatgcgctg gggcatgccc ccgcaggacg ccttcaactc
     8461 ccactggctg gtgcgggacc ttcgagggaa gagcgagaag gagtttagag cctatgtgtc
     8521 cctcttcatg cggcacctgt gtgagccggg ggcggatggt gcagagacct tcgcagacgg
     8581 cgtgccccgg gagggcctct ccaggcagca cgtgctgacc cgcatcgggg tcatgtcact
     8641 agttaggaag aaggttcagg agtttgagca tgtcaacggg aagtacagca ccccagactt
     8701 gatccctgag gggcccgagg ggaagaagtc gggcgaggtg atctcctcgg accccaacac
     8761 accagtgccc gccagccctg cccacctcct gccagccccg ctgggcctgc cagacaaaat
     8821 ggaagcccag ctgggctaca tggatgagaa agaccccggg gcacagaagc caaggcagcc
     8881 cctggaagtc caggcccttc cagccgcctt ggatagagtg gagagtgagg acaagcacga
     8941 gagcccagcc agcaaggaga gagcccgaga ggagcggcca gaggagacgg agaaggcccc
     9001 gccctccccg gagcagctgc cgagagagga ggtgcttcct gagaaggaga agatcctgga
     9061 caagctggag ctgagcttga tccacagcag aggggacagt tccgaactca ggccagatga
     9121 caccaaggct gaggagaagg agcccattga aacacagcaa aatggtgaca aagaggaaga
     9181 tgacgagggg aagaaggagg acaagaaggg gaaattcaag ttcatgttca acatcgcgga
     9241 cgggggcttc acggagttgc acacgctgtg gcagaacgag gagcgggctg ctgtatcctc
     9301 tgggaaaatc tacgacatct ggcaccggcg ccatgactac tggctgctgg cgggcatcgt
     9361 gacgcacggc tacgcccgct ggcaggacat ccagaatgac ccacggtaca tgatcctcaa
     9421 cgagcccttc aagtctgagg tccacaaggg caactacctg gagatgaaga acaagttcct
     9481 ggcccgcagg tttaagctgc tggagcaggc gttggtcatt gaggagcagc tccggagggc
     9541 cgcgtacctg aacatgacgc aggaccccaa ccaccccgcc atggccctca acgcccgcct
     9601 ggctgaagtg gagtgcctcg ccgagagcca ccagcacctg tccaaggagt cccttgctgg
     9661 gaacaagcct gccaatgccg tcctgcacaa ggtcctgaac cagctggagg agctgctgag
     9721 cgacatgaag gccgacgtga cccggctgcc atccatgctg tcccgcatcc ccccggtggc
     9781 cgcccggctg cagatgtcgg agcgcagcat cctgagccgc ctgaccaacc gcgccgggga
     9841 ccccaccatc cagcagggcg ctttcggctc ctcccagatg tacagcaaca actttgggcc
     9901 caacttccgg ggccctggac cgggagggat tgtcaactac aaccagatgc ccctggggcc
     9961 ctatgtgacc gatatctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
    10021 gctttatata tcttgtggaa aggacgaggt attaatttgg ttgcacatca aacgcgttaa
    10081 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
    10681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    10741 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    10801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10981 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    11041 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11161 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    11221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11281 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    11341 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    11401 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    11461 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    11521 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    11581 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    11641 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    11701 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    11761 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    11821 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    11881 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    11941 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    12001 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    12061 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    12121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    12181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    12241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    12301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    12361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    12421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    12481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    12541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    12601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    12661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    12721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    12781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    12841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    12901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    12961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    13021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    13081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    13141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    13201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    13261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    13321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    13381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    13441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    13501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    13561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    13621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    13681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    13741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    13801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    13861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    13921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    13981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    14041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    14101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    14161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    14221 agctgcaagc
//
