LOCUS       sequence-396151-       15694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1108
FEATURES             Location/Qualifiers
     source          1..15694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8981..8990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11551..12139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11604..11624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12022..12033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12211..12444
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12522..12643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12559..12578)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12613..12632
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12683..12818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12838..12857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12839..12857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12867..12884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12867..12883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12876..12898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13025..13480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13112..13131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13322..13343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13506..13610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13611..13679,13680..14471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13829..13848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14642..15230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15131..15150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15384..15401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15518..15539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15554..15559,15560..15577,15578..15584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15592..15608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15597..15619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15616..15632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15616..15632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15651..15671
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15653..15671
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggct gagaacttgc tggacggacc
     4141 gcccaacccc aaaagagcca aactcagctc gcccggtttc tcggcgaatg acagcacaga
     4201 ttttggatca ttgtttgact tggaaaatga tcttcctgat gagctgatac ccaatggagg
     4261 agaattaggc cttttaaaca gtgggaacct tgttccagat gctgcttcca aacataaaca
     4321 actgtcggag cttctacgag gaggcagcgg ctctagtatc aacccaggaa taggaaatgt
     4381 gagcgccagc agccccgtgc agcagggcct gggtggccag gctcaagggc agccgaacag
     4441 tgctaacatg gccagcctca gtgccatggg caagagccct ctgagccagg gagattcttc
     4501 agcccccagc ctgcctaaac aggcagccag cacctctggg cccacccccg ctgcctccca
     4561 agcactgaat ccgcaagcac aaaagcaagt ggggctggcg actagcagcc ctgccacgtc
     4621 acagactgga cctggtatct gcatgaatgc taactttaac cagacccacc caggcctcct
     4681 caatagtaac tctggccata gcttaattaa tcaggcttca caagggcagg cgcaagtcat
     4741 gaatggatct cttggggctg ctggcagagg aaggggagct ggaatgccgt accctactcc
     4801 agccatgcag ggcgcctcga gcagcgtgct ggctgagacc ctaacgcagg tttccccgca
     4861 aatgactggt cacgcgggac tgaacaccgc acaggcagga ggcatggcca agatgggaat
     4921 aactgggaac acaagtccat ttggacagcc ctttagtcaa gctggagggc agccaatggg
     4981 agccactgga gtgaaccccc agttagccag caaacagagc atggtcaaca gtttgcccac
     5041 cttccctaca gatatcaaga atacttcagt caccaacgtg ccaaatatgt ctcagatgca
     5101 aacatcagtg ggaattgtac ccacacaagc aattgcaaca ggccccactg cagatcctga
     5161 aaaacgcaaa ctgatacagc agcagctggt tctactgctt catgctcata agtgtcagag
     5221 acgagagcaa gcaaacggag aggttcgggc ctgctcgctc ccgcattgtc gaaccatgaa
     5281 aaacgttttg aatcacatga cgcattgtca ggctgggaaa gcctgccaag ttgcccattg
     5341 tgcatcttca cgacaaatca tctctcattg gaagaactgc acacgacatg actgtcctgt
     5401 ttgcctccct ttgaaaaatg ccagtgacaa gcgaaaccaa caaaccatcc tggggtctcc
     5461 agctagtgga attcaaaaca caattggttc tgttggcaca gggcaacaga atgccacttc
     5521 tttaagtaac ccaaatccca tagaccccag ctccatgcag cgagcctatg ctgctctcgg
     5581 actcccctac atgaaccagc cccagacgca gctgcagcct caggttcctg gccagcaacc
     5641 agcacagcct caaacccacc agcagatgag gactctcaac cccctgggaa ataatccaat
     5701 gaacattcca gcaggaggaa taacaacaga tcagcagccc ccaaacttga tttcagaatc
     5761 agctcttccg acttccctgg gggccacaaa cccactgatg aacgatggct ccaactctgg
     5821 taacattgga accctcagca ctataccaac agcagctcct ccttctagca ccggtgtaag
     5881 gaaaggctgg cacgaacatg tcactcagga cctgcggagc catctagtgc ataaactcgt
     5941 ccaagccatc ttcccaacac ctgatcccgc agctctaaag gatcgccgca tggaaaacct
     6001 ggtagcctat gctaagaaag tggaagggga catgtacgag tctgccaaca gcagggatga
     6061 atattatcac ttattagcag agaaaatcta caagatacaa aaagaactag aagaaaaacg
     6121 gaggtcgcgt ttacataaac aaggcatctt ggggaaccag ccagccttac cagccccggg
     6181 ggctcagccc cctgtgattc cacaggcaca acctgtgaga cctccaaatg gacccctgtc
     6241 cctgccagtg aatcgcatgc aagtttctca agggatgaat tcatttaacc ccatgtcctt
     6301 ggggaacgtc cagttgccac aagcacccat gggacctcgt gcagcctccc caatgaacca
     6361 ctctgtccag atgaacagca tgggctcagt gccagggatg gccatttctc cttcccgaat
     6421 gcctcagcct ccgaacatga tgggtgcaca caccaacaac atgatggccc aggcgcccgc
     6481 tcagagccag tttctgccac agaaccagtt cccgtcatcc agcggggcga tgagtgtggg
     6541 catggggcag ccgccagccc aaacaggcgt gtcacaggga caggtgcctg gtgctgctct
     6601 tcctaaccct ctcaacatgc tggggcctca ggccagccag ctaccttgcc ctccagtgac
     6661 acagtcacca ctgcacccaa caccgcctcc tgcttccacg gctgctggca tgccatctct
     6721 ccagcacacg acaccacctg ggatgactcc tccccagcca gcagctccca ctcagccatc
     6781 aactcctgtg tcgtcttccg ggcagactcc caccccgact cctggctcag tgcccagtgc
     6841 tacccaaacc cagagcaccc ctacagtcca ggcagcagcc caggcccagg tgaccccgca
     6901 gcctcaaacc ccagttcagc ccccgtctgt ggctacccct cagtcatcgc agcaacagcc
     6961 gacgcctgtg cacgcccagc ctcctggcac accgctttcc caggcagcag ccagcattga
     7021 taacagagtc cctaccccct cctcggtggc cagcgcagaa accaattccc agcagccagg
     7081 acctgacgta cctgtgctgg aaatgaagac ggagacccaa gcagaggaca ctgagcccga
     7141 tcctggtgaa tccaaagggg agcccaggtc tgagatgatg gaggaggatt tgcaaggagc
     7201 ttcccaagtt aaagaagaaa cagacatagc agagcagaaa tcagaaccaa tggaagtgga
     7261 tgaaaagaaa cctgaagtga aagtagaagt taaagaggaa gaagagagta gcagtaacgg
     7321 cacagcctct cagtcaacat ctccttcgca gccgcgcaaa aaaatcttta aaccagagga
     7381 gttacgccag gccctcatgc caaccctaga agcactgtat cgacaggacc cagagtcatt
     7441 acctttccgg cagcctgtag atccccagct cctcggaatt ccagactatt ttgacatcgt
     7501 aaagaatccc atggacctct ccaccatcaa gcggaagctg gacacagggc aataccaaga
     7561 gccctggcag tacgtggacg acgtctggct catgttcaac aatgcctggc tctataatcg
     7621 caagacatcc cgagtctata agttttgcag taagcttgca gaggtctttg agcaggaaat
     7681 tgaccctgtc atgcagtccc ttggatattg ctgtggacgc aagtatgagt tttccccaca
     7741 gactttgtgc tgctatggga agcagctgtg taccattcct cgcgatgctg cctactacag
     7801 ctatcagaat aggtatcatt tctgtgagaa gtgtttcaca gagatccagg gcgagaatgt
     7861 gaccctgggt gacgaccctt cacagcccca gacgacaatt tcaaaggatc agtttgaaaa
     7921 gaagaaaaat gataccttag accccgaacc tttcgttgat tgcaaggagt gtggccggaa
     7981 gatgcatcag atttgcgttc tgcactatga catcatttgg ccttcaggtt ttgtgtgcga
     8041 caactgcttg aagaaaactg gcagacctcg aaaagaaaac aaattcagtg ctaagaggct
     8101 gcagaccaca agactgggaa accacttgga agaccgagtg aacaaatttt tgcggcgcca
     8161 gaatcaccct gaagccgggg aggtttttgt ccgagtggtg gccagctcag acaagacggt
     8221 ggaggtcaag cccgggatga agtcacggtt tgtggattct ggggaaatgt ctgaatcttt
     8281 cccatatcga accaaagctc tgtttgcttt tgaggaaatt gacggcgtgg atgtctgctt
     8341 ttttggaatg cacgtccaag aatacggctc tgattgcccc cctccaaaca cgaggcgtgt
     8401 gtacatttct tatctggata gtattcattt cttccggcca cgttgcctcc gcacagccgt
     8461 ttaccatgag atccttattg gatatttaga gtatgtgaag aaattagggt atgtgacagg
     8521 gcacatctgg gcctgtcctc caagtgaagg agatgattac atcttccatt gccacccacc
     8581 tgatcaaaaa atacccaagc caaaacgact gcaggagtgg tacaaaaaga tgctggacaa
     8641 ggcgtttgca gagcggatca tccatgacta caaggatatt ttcaaacaag caactgaaga
     8701 caggctcacc agtgccaagg aactgcccta ttttgaaggt gatttctggc ccaatgtgtt
     8761 agaagagagc attaaggaac tagaacaaga agaagaggag aggaaaaagg aagagagcac
     8821 tgcagccagt gaaaccactg agggcagtca gggcgacagc aagaatgcca agaagaagaa
     8881 caacaagaaa accaacaaga acaaaagcag catcagccgc gccaacaaga agaagcccag
     8941 catgcccaac gtgtccaatg acctgtccca gaagctgtat gccaccatgg agaagcacaa
     9001 ggaggtcttc ttcgtgatcc acctgcacgc tgggcctgtc atcaacaccc tgccccccat
     9061 cgtcgacccc gaccccctgc tcagctgtga cctcatggat gggcgcgacg ccttcctcac
     9121 cctcgccaga gacaagcact gggagttctc ctccttgcgc cgctccaagt ggtccacgct
     9181 ctgcatgctg gtggagctgc acacccaggg ccaggaccgc tttgtctaca cctgcaacga
     9241 gtgcaagcac cacgtggaga cgcgctggca ctgcactgtg tgcgaggact acgacctctg
     9301 catcaactgc tataacacga agagccatgc ccataagatg gtgaagtggg ggctgggcct
     9361 ggatgacgag ggcagcagcc agggcgagcc acagtcaaag agcccccagg agtcacgccg
     9421 gctgagcatc cagcgctgca tccagtcgct ggtgcacgcg tgccagtgcc gcaacgccaa
     9481 ctgctcgctg ccatcctgcc agaagatgaa gcgggtggtg cagcacacca agggctgcaa
     9541 acgcaagacc aacgggggct gcccggtgtg caagcagctc atcgccctct gctgctacca
     9601 cgccaagcac tgccaagaaa acaaatgccc cgtgcccttc tgcctcaaca tcaaacacaa
     9661 gctccgccag cagcagatcc agcaccgcct gcagcaggcc cagctcatgc gccggcggat
     9721 ggccaccatg aacacccgca acgtgcctca gcagagtctg ccttctccta cctcagcacc
     9781 gcccgggacc cccacacagc agcccagcac accccagacg ccgcagcccc ctgcccagcc
     9841 ccaaccctca cccgtgagca tgtcaccagc tggcttcccc agcgtggccc ggactcagcc
     9901 ccccaccacg gtgtccacag ggaagcctac cagccaggtg ccggcccccc cacccccggc
     9961 ccagccccct cctgcagcgg tggaagcggc tcggcagatc gagcgtgagg cccagcagca
    10021 gcagcacctg taccgggtga acatcaacaa cagcatgccc ccaggacgca cgggcatggg
    10081 gaccccgggg agccagatgg cccccgtgag cctgaatgtg ccccgaccca accaggtgag
    10141 cgggcccgtc atgcccagca tgcctcccgg gcagtggcag caggcgcccc ttccccagca
    10201 gcagcccatg ccaggcttgc ccaggcctgt gatatccatg caggcccagg cggccgtggc
    10261 tgggccccgg atgcccagcg tgcagccacc caggagcatc tcacccagcg ctctgcaaga
    10321 cctgctgcgg accctgaagt cgcccagctc ccctcagcag caacagcagg tgctgaacat
    10381 tctcaaatca aacccgcagc taatggcagc tttcatcaaa cagcgcacag ccaagtacgt
    10441 ggccaatcag cccggcatgc agccccagcc tggcctccag tcccagcccg gcatgcaacc
    10501 ccagcctggc atgcaccagc agcccagcct gcagaacctg aatgccatgc aggctggcgt
    10561 gccgcggccc ggtgtgcctc cacagcagca ggcgatggga ggcctgaacc cccagggcca
    10621 ggccttgaac atcatgaacc caggacacaa ccccaacatg gcgagtatga atccacagta
    10681 ccgagaaatg ttacggaggc agctgctgca gcagcagcag caacagcagc agcaacaaca
    10741 gcagcaacag cagcagcagc aagggagtgc cggcatggct gggggcatgg cggggcacgg
    10801 ccagttccag cagcctcaag gacccggagg ctacccaccg gccatgcagc agcagcagcg
    10861 catgcagcag catctccccc tccagggcag ctccatgggc cagatggcgg ctcagatggg
    10921 acagcttggc cagatggggc agccggggct gggggcagac agcaccccca acatccagca
    10981 agccctgcag cagcggattc tgcagcaaca gcagatgaag cagcagattg ggtccccagg
    11041 ccagccgaac cccatgagcc cccagcaaca catgctctca ggacagccac aggcctcgca
    11101 tctccctggc cagcagatcg ccacgtccct tagtaaccag gtgcggtctc cagcccctgt
    11161 ccagtctcca cggccccagt cccagcctcc acattccagc ccgtcaccac ggatacagcc
    11221 ccagccttcg ccacaccacg tctcacccca gactggttcc ccccaccccg gactcgcagt
    11281 caccatggcc agctccatag atcagggaca cttggggaac cccgaacaga gtgcaatgct
    11341 cccccagctg aacaccccca gcaggagtgc gctgtccagc gaactgtccc tggtcgggga
    11401 caccacgggg gacacgctag agaagtttgt ggagggcttg tagtaatgaa ctagtccgta
    11461 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aatccgacca
    11521 ctgactatcg agtccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
    11581 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    11641 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    11701 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    11761 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    11821 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    11881 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    11941 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    12001 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    12061 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    12121 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    12181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    12241 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    12301 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    12361 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    12421 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
    12481 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    12541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    12601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    12661 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    12721 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    12781 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    12841 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    12901 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    12961 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    13021 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    13081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    13141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    13201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    13261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    13321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    13381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    13441 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    13501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    13561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    13621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    13681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    13741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    13801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    13861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    13921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    13981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    14041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    14101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    14161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    14221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    14281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    14341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    14401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    14461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    14521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    14581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    14641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    14701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    14761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    14821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    14881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    14941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    15001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    15061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    15121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    15181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    15241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    15301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    15361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    15421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    15481 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    15541 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    15601 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    15661 ctcactaaag ggaacaaaag ctggagctgc aagc
//
