LOCUS       sequence-396167-       13066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1153
FEATURES             Location/Qualifiers
     source          1..13066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     RBS             5091..5099
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    8923..9511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8976..8996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9394..9405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9583..9816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9894..10015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9931..9950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9985..10004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10055..10190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10210..10229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10211..10229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10239..10256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10239..10255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10248..10270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10397..10852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10484..10503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10694..10715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10878..10982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10983..11051,11052..11843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11201..11220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12014..12602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12503..12522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12756..12773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12890..12911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12926..12931,12932..12949,12950..12956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12964..12980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12969..12991
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12988..13004
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12988..13004
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13023..13043
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13025..13043
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaca gcggacaaag acaaagacaa
     4141 agacaaagag aaggaccggg accgagaccg ggaccgagag agagagaaaa gagacaaagc
     4201 aagagagagt gagaattcaa ggccacgccg gagctgtacc ttggaaggag gagccaaaaa
     4261 ttatgctgag agtgatcaca gtgaagacga ggacaatgac aacaatagtg ccaccgcaga
     4321 ggagtccacg aagaagaata agaagaaacc accgaaaaaa aagtctcgtt atgaaaggac
     4381 agataccggt gagataacat cctacatcac tgaagatgat gtggtctaca gaccaggaga
     4441 ctgtgtgtat atcgagagtc ggaggccaaa cacaccgtat ttcatctgta gcattcaaga
     4501 cttcaaactg gtccacaact cccaggcctg ttgcagatct ccaactcctg ctttgtgtga
     4561 ccccccagca tgctctctgc cggtggcatc acagccacca cagcatcttt ctgaagccgg
     4621 gagagggcct gtagggagta agagggacca tctcctcatg aacgtcaaat ggtactaccg
     4681 tcaatctgag gttccagatt ctgtgtatca gcatttggtt caggatcgac ataatgaaaa
     4741 tgactctgga agagaacttg tcattacaga cccagttatc aagaaccgag agctcttcat
     4801 ttctgattac gttgacactt accatgctgc tgcccttaga gggaagtgta acatctccca
     4861 tttttctgac atatttgctg ctagagagtt taaagcccga gtggattcat ttttctacat
     4921 attaggatat aaccctgaga caaggaggct gaacagtacc cagggggaga ttcgtgtcgg
     4981 tcctagtcat caggccaaac ttccagatct gcaaccattt ccttctccag atggtgatac
     5041 agtgacccaa catgaggaac tggtctggat gcctggagtt aacgactgtg acctccttat
     5101 gtacttgagg gcagcaagga gcatggcggc atttgcagga atgtgtgatg gaggctctac
     5161 agaggacggc tgtgtcgcag cctctcggga tgacaccact ctgaatgcac tgaacacact
     5221 gcatgaaagc ggttacgatg ctggcaaagc cctgcagcgc ctggtgaaga agcctgtgcc
     5281 caagctcatc gagaagtgct ggaccgagga cgaagtgaaa cgcttcgtta agggactcag
     5341 gcagtacggg aagaacttct tcagaattag aaaggagctg cttcccaata aggaaacagg
     5401 ggagctgatc accttctatt actattggaa gaagaccccc gaagcagcca gctcccgagc
     5461 ccatcgtagg caccgcaggc aggccgtgtt caggaggatt aagactcgca ccgcgtccac
     5521 acccgtcaac acaccctcca gacccccgtc cagtgaattc ttggacctaa gttcagccag
     5581 tgaagatgac ttcgacagtg aggacagtga gcaggagctg aaggggtacg cctgccgcca
     5641 ctgcttcacc accacctcca aagattggca ccacggaggc cgggagaaca tcctgctttg
     5701 caccgactgt cgcatccact tcaagaaata cggtgagctc ccgcccattg agaagcccgt
     5761 ggacccgcca ccgtttatgt tcaaacccgt caaggaagag gatgatgggc tcagtgggaa
     5821 gcatagcatg aggacacggc ggagtcgggg ctcgatgtcg acactacgca gtggtcggaa
     5881 gaagcagcca gccagccctg atggtcgcac ctcacccatc aatgaagaca tccgctccag
     5941 cggccggaac tcccccagcg ctgccagtac ctccagcaat gacagtaaag cagagacagt
     6001 gaagaagtcg gccaagaagg tgaaggagga agcctcttcc cctcttaaga gtaacaaacg
     6061 ccagcgggag aaggtggcct ctgatacgga ggaggctgac aggaccagct ccaagaagac
     6121 aaaaacgcag gagatcagca ggcccaactc gccatctgaa ggtgagggag agagttcaga
     6181 cagtcgcagc gtcaacgatg agggtagcag tgaccccaaa gacatcgacc aggacaatcg
     6241 cagcacgtcc ccgagcatcc ccagccccca ggacaatgag agtgactcgg actcgtcagc
     6301 ccagcagcag atgctgcagg cccagccccc agccttgcag gctcccactg gggtcacccc
     6361 agctccctcc tcagctcctc cagggacccc tcagctgccc acgccagggc ccacgccctc
     6421 tgccactgca gttcccccac agggctcccc cacggcctcc caggccccta accagccaca
     6481 ggctcccaca gcgcctgttc cccacaccca catccaacag gcaccggcct tgcaccccca
     6541 gcggccgccc tcaccgcatc ccccgccgca tccctcgcca catcccccgc tgcagcctct
     6601 gactgggtcg gcgggccagc cttctgcacc ctctcatgcc cagcccccac tgcacggtca
     6661 gggcccaccc ggccctcaca gcctgcaggc tgggcccctg ctgcagcacc caggcccccc
     6721 acagcccttt ggcctccctc cccaggcctc ccaaggccag gcccctctgg ggacctcccc
     6781 agcagcagcg taccctcaca cctccctgca gctgccagcc tctcagtcag cgctgcagtc
     6841 ccaacagcct ccacgggagc agcccctgcc accagcgccc ttggccatgc cccacatcaa
     6901 gcccccgcct accactccca tcccccagct gccggcgcca caggcccaca agcaccctcc
     6961 ccacctctcg gggccctcac ccttctccat gaatgccaac ctgcctcccc ctccagccct
     7021 gaagcccctg agctccctgt ccacacatca ccccccgtcg gctcaccccc cacccctgca
     7081 actcatgcct cagagccagc cattgccctc ctcgcccgcc cagccccccg ggctgaccca
     7141 gagccagaac ctgcccccgc cccctgcctc ccacccccct acaggcctcc accaggtggc
     7201 cccccaaccc ccgtttgctc agcacccctt tgtccctgga ggccctcctc ccatcacccc
     7261 tccgacctgc ccctccacct ctaccccacc ggcgggacct ggcacctcgg cccagccacc
     7321 ctgctctggt gcggcggctt caggaggcag catagcgggg gggtcgtcct gcccactccc
     7381 caccgtccag atcaaggagg aggctctgga cgacgctgag gagcctgaga gcccccctcc
     7441 cccaccaagg agcccgtccc cggagcccac tgtggtggac acccccagtc acgccagcca
     7501 gtcagctagg ttctacaaac acctggaccg gggctacaac tcgtgtgccc ggacagacct
     7561 gtacttcatg cctctggccg ggtccaagct ggccaagaag agggaggagg ccattgagaa
     7621 ggccaagcgc gaggctgagc agaaagcccg agaggagcga gagcgggaga aggagaagga
     7681 gaaggagcgg gagcgggagc gagagcggga gcgcgaggca gagcgggcgg ctaaggcgtc
     7741 cagctcagcg catgaaggtc gcctcagtga cccacagctc agtggtcctg gccacatgcg
     7801 gccatccttc gagccaccac caaccaccat tgctgctgtg cccccctaca tcgggcccga
     7861 cacacctgcc cttcggactc tgagcgagta cgcccggccc cacgtcatgt cgcccaccaa
     7921 ccgcaaccac cccttctaca tgccccttaa ccccacggac cccctgctgg cctaccacat
     7981 gcctggcctc tacaacgtcg accccaccat ccgcgagcgg gagctccggg agcgggagat
     8041 ccgagagcgg gagatccgag agcgggagct gcgggagagg atgaagccgg gcttcgaggt
     8101 gaagccccca gagctggacc ccctgcaccc agccgccaac cccatggagc actttgcccg
     8161 gcacagcgcc ctcaccatcc ccccgaccgc cgggccccac ccttttgctt ctttccaccc
     8221 gggcctgaac cccttggaga gggagagact ggccctggcg ggcccccagc tgcggcccga
     8281 gatgagctac cctgacagac tggcagccga gcgtatccac gcagagcgca tggcatcgct
     8341 gaccagcgat cccctggccc gactgcagat gttcaacgtg actccgcacc atcaccagca
     8401 ctctcacatt cactcccacc tccacctcca ccagcaggac cccctccacc aaggttcagc
     8461 aggccccgtt cacccgctgg tcgaccccct gactgccggt ccccacctgg ctcgcttccc
     8521 ctacccgcct ggcactctcc ccaaccctct gcttggacag cccccacacg agcacgagat
     8581 gcttcgccac ccagttttcg gcacccccta cccccgtgac ctgcctgggg ccatcccacc
     8641 ccccatgtca gcagcccacc agctgcaggc catgcatgcc cagtcggccg agctgcagag
     8701 actggccatg gagcagcagt ggctgcatgg acacccccac atgcatggtg gccacctacc
     8761 aagtcaggaa gattattaca gtcgactgaa gaaagaaggt gacaagcagt tatagtaatg
     8821 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     8881 cgaagacaga ttccgaaagt ctacagcacg cgttaagtcg acaatcaacc tctggattac
     8941 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9001 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9061 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9121 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9181 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9241 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9301 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9361 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9421 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9481 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     9541 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9601 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     9841 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     9901 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9961 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10021 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    10081 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10141 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    10201 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10261 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10321 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10381 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10441 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10501 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10561 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10621 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10681 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10741 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10801 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10861 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10921 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10981 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    11041 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    11101 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11161 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11221 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11281 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11341 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11401 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11461 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11521 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11581 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11641 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11701 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11761 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11821 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11881 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11941 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12001 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12061 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    12181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12901 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12961 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    13021 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
