LOCUS       sequence-396169-       15742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0703
FEATURES             Location/Qualifiers
     source          1..15742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11599..12187
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11652..11672)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12070..12081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12259..12492
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12570..12691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12607..12626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12661..12680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12731..12866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12886..12905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12887..12905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12915..12932)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12915..12931)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12924..12946)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13073..13528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13160..13179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13370..13391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13554..13658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13659..13727,13728..14519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13877..13896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14690..15278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15179..15198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15432..15449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15566..15587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15602..15607,15608..15625,15626..15632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15640..15656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15645..15667
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15664..15680
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15664..15680
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15699..15719
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15701..15719
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtcg ggatccacac agcctgtggc
     4141 acagacgtgg agggccactg agccccgcta cccgccccac agcctttcct acccagtgca
     4201 gatcgcccgg acgcacacgg acgtcgggct cctggagtac cagcaccact cccgcgacta
     4261 tgcctcccac ctgtcgcccg gctccatcat ccagccccag cggcggaggc cctccctgct
     4321 gtctgagttc cagcccggga atgaacggtc ccaggagctc cacctgcggc cagagtccca
     4381 ctcatacctg cccgagctgg ggaagtcaga gatggagttc attgaaagca agcgccctcg
     4441 gctagagctg ctgcctgacc ccctgctgcg accgtcaccc ctgctggcca cgggccagcc
     4501 tgcgggatct gaagacctca ccaaggaccg tagcctgacg ggcaagctgg aaccggtgtc
     4561 tccccccagc cccccgcaca ctgaccctga gctggagctg gtgccgccac ggctgtccaa
     4621 ggaggagctg atccagaaca tggaccgcgt ggaccgagag atcaccatgg tagagcagca
     4681 gatctctaag ctgaagaaga agcagcaaca gctggaggag gaggctgcca agccgcccga
     4741 gcctgagaag cccgtgtcac cgccgcccat cgagtcgaag caccgcagcc tggtgcagat
     4801 catctacgac gagaaccgga agaaggctga agctgcacat cggattctgg aaggcctggg
     4861 gccccaggtg gagctgccgc tgtacaacca gccctccgac acccggcagt atcatgagaa
     4921 catcaaaata aaccaggcga tgcggaagaa gctaatcttg tacttcaaga ggaggaatca
     4981 cgctcggaaa caatgggagc agaagttctg ccagcgctat gaccagctca tggaggcctg
     5041 ggagaagaag gtggagcgca tcgagaacaa cccccggcgg cgggccaagg agagcaaggt
     5101 gcgcgagtac tacgagaagc agttccctga gatccgcaag cagcgcgagc tgcaggagcg
     5161 catgcagagg gtgggccagc ggggcagtgg gctgtccatg tcggccgccc gcagcgagca
     5221 cgaggtgtca gagatcatcg atggcctctc agagcaggag aacctggaga agcagatgcg
     5281 ccagctggcc gtgatcccgc ccatgctgta cgacgctgac cagcagcgca tcaagttcat
     5341 caacatgaac gggcttatgg ccgaccccat gaaggtgtac aaagaccgcc aggtcatgaa
     5401 catgtggagt gagcaggaga aggagacctt ccgggagaag ttcatgcagc atcccaagaa
     5461 ctttggcctg atcgcatcat tcctggagag gaagacagtg gctgagtgcg tcctctatta
     5521 ctacctgact aagaagaatg agaactataa gagcctggtg agacggagct atcggcgccg
     5581 cggcaagagc cagcagcagc aacaacagca gcagcagcag cagcagcagc agcagcagca
     5641 gcccatgccc cgcagcagcc aggaggagaa agatgagaag gagaaggaaa aggaggcgga
     5701 gaaggaggag gagaagccgg aggtggagaa cgacaaggaa gacctcctca aggagaagac
     5761 agacgacacc tcaggggagg acaacgacga gaaggaggct gtggcctcca aaggccgcaa
     5821 aactgccaac agccagggaa gacgcaaagg ccgcatcacc cgctcaatgg ctaatgaggc
     5881 caacagcgag gaggccatca ccccccagca gagcgccgag ctggcctcca tggagctgaa
     5941 tgagagttct cgctggacag aagaagaaat ggaaacagcc aagaaaggtc tcctggaaca
     6001 cggccgcaac tggtcggcca tcgcccggat ggtgggctcc aagactgtgt cgcagtgtaa
     6061 gaacttctac ttcaactaca agaagaggca gaacctcgat gagatcttgc agcagcacaa
     6121 gctgaagatg gagaaggaga ggaacgcgcg gaggaagaag aagaaagcgc cggcggcggc
     6181 cagcgaggag gctgcattcc cgcccgtggt ggaggatgag gagatggagg cgtcgggcgt
     6241 gagcggaaat gaggaggaga tggtggagga ggctgaagcc actgtcaaca acagctcaga
     6301 caccgagagc atcccctctc ctcacactga ggccgccaag gacacagggc agaatgggcc
     6361 caagccccca gccaccctgg gcgccgacgg gccaccccca gggccaccca ccccaccacc
     6421 ggaggacatc ccggccccca ctgagcccac cccggcctct gaagccaccg gagcccctac
     6481 gcccccacca gcacccccat cgccctctgc acctcctcct gtggtcccca aggaggagaa
     6541 ggaggaggag accgcagcag cgcccccagt ggaggagggg gaggagcaga agccccccgc
     6601 ggctgaggag ctggcagtgg acacagggaa ggccgaggag cccgtcaaga gcgagtgcac
     6661 ggaggaagcc gaggaggggc cggccaaggg caaggacgcg gaggccgctg aggccacggc
     6721 cgagggggcg ctcaaggcag agaagaagga gggcgggagc ggcagggcca ccacagccaa
     6781 gagctcgggc gccccccagg acagcgactc cagtgctacc tgcagtgcag acgaggtgga
     6841 tgaggccgag ggcggcgaca agaaccggct gctgtcccca aggcccagcc tcctcacccc
     6901 gactggcgac ccccgggcca atgcctcacc ccagaagcca ctggacctga agcagctgaa
     6961 gcagcgagcg gctgccatcc cccccatcca ggtcaccaaa gtccatgagc ccccccggga
     7021 ggacgcagct cccaccaagc cagctccccc agccccaccg ccaccgcaaa acctgcagcc
     7081 ggagagcgac gcccctcagc agcctggcag cagcccccgg ggcaagagca ggagcccggc
     7141 accccccgcc gacaaggagg cagagaagcc tgtgttcttc ccagccttcg cagccgaggc
     7201 ccagaagctg cctggggacc ccccttgctg gacttccggc ctgcccttcc ccgtgccccc
     7261 ccgtgaggtg atcaaggcct ccccgcatgc cccggacccc tcagccttct cctacgctcc
     7321 acctggtcac ccactgcccc tgggcctcca tgacactgcc cggcccgtcc tgccgcgccc
     7381 acccaccatc tccaacccgc ctcccctcat ctcctctgcc aagcacccca gcgtcctcga
     7441 gaggcaaata ggtgccatct cccaaggaat gtcggtccag ctccacgtcc cgtactcaga
     7501 gcatgccaag gccccggtgg gccctgtcac catggggctg cccctgccca tggaccccaa
     7561 aaagctggca cccttcagcg gagtgaagca ggagcagctg tccccacggg gccaggctgg
     7621 gccaccggag agcctggggg tgcccacagc ccaggaggcg tccgtgctga gagggacagc
     7681 tctgggctca gttccgggcg gaagcatcac caaaggcatt cccagcacac gggtgccctc
     7741 ggacagcgcc atcacatacc gcggctccat cacccacggc acgccagctg acgtcctgta
     7801 caagggcacc atcaccagga tcatcggcga ggacagcccg agtcgcttgg accgcggccg
     7861 ggaggacagc ctgcccaagg gccacgtcat ctacgaaggc aagaagggcc acgtcttgtc
     7921 ctatgagggt ggcatgtctg tgacccagtg ctccaaggag gacggcagaa gcagctcagg
     7981 acccccccat gagacggccg cccccaagcg cacctatgac atgatggagg gccgcgtggg
     8041 cagagccatc tcctcagcca gcatcgaagg tctcatgggc cgtgccatcc cgccggagcg
     8101 acacagcccc caccacctca aagagcagca ccacatccgc gggtccatca cacaagggat
     8161 ccctcggtcc tacgtggagg cacaggagga ctacctgcgt cgggaggcca agctcctaaa
     8221 gcgggagggc acgcctccgc ccccaccgcc ctcacgggac ctgaccgagg cctacaagac
     8281 gcaggccctg ggccccctga agctgaagcc ggcccatgag ggcctggtgg ccacggtgaa
     8341 ggaggcgggc cgctccatcc atgagatccc gcgcgaggag ctgcggcaca cgcccgagct
     8401 gcccctggcc ccgcggccgc tcaaggaggg ctccatcacg cagggcaccc cgctcaagta
     8461 cgacaccggc gcgtccacca ctggctccaa aaagcacgac gtacgctccc tcatcggcag
     8521 ccccggccgg acgttcccac ccgtgcaccc gctggatgtg atggccgacg cccgggcact
     8581 ggaacgtgcc tgctacgagg agagcctgaa gagccggcca gggaccgcca gcagctcggg
     8641 gggctccatt gcgcgcggcg ccccggtcat tgtgcctgag ctgggtaagc cgcggcagag
     8701 ccccctgacc tatgaggacc acggggcacc ctttgccggc cacctcccac gaggttcgcc
     8761 cgtgaccacg cgggagccca cgccgcgcct gcaggagggc agcctttcgt ccagcaaggc
     8821 atcccaggac cgaaagctga cgtcgacgcc tcgtgagatc gccaagtccc cgcacagcac
     8881 cgtgcccgag caccacccac accccatctc gccctatgag cacctgcttc ggggcgtgag
     8941 tggcgtggac ctgtatcgca gccacatccc cctggccttc gaccccacct ccataccccg
     9001 cggcatccct ctggacgcag ccgctgccta ctacctgccc cgacacctgg cccccaaccc
     9061 cacctacccg cacctgtacc caccctacct catccgcggc taccccgaca cggcggcgct
     9121 ggagaaccgg cagaccatca tcaatgacta catcacctcg cagcagatgc accacaacgc
     9181 ggccaccgcc atggcccagc gagctgatat gctgaggggc ctctcgcccc gcgagtcctc
     9241 gctggcactc aactacgctg cgggtccccg aggcatcatc gacctgtccc aagtgccaca
     9301 cctgcctgtg ctcgtgcccc cgacaccagg caccccagcc accgccatgg accgccttgc
     9361 ctacctcccc accgcgcccc agcccttcag cagccgccac agcagctccc cactctcccc
     9421 aggaggtcca acacacttga caaaaccaac caccacgtcc tcgtccgagc gggagcgaga
     9481 ccgggatcga gagcgggacc gggatcggga gcgggaaaag tccatcctca cgtccaccac
     9541 gacggtggag cacgcaccca tctggagacc tggtacagag cagagcagcg gcagcagcgg
     9601 cgggggtggg ggcagcagca gccgccccgc ctcccactcc catgcccacc agcactcgcc
     9661 catctcccct cggacccagg atgccctcca gcagagaccc agtgtgcttc acaacacagg
     9721 catgaagggt atcatcaccg ctgtggagcc cagcacgccc acggtcctga ggtccacctc
     9781 cacctcctca cccgttcgcc cggctgccac attcccacct gccacccact gcccactggg
     9841 cggcaccctc gatggggtct accctaccct catggagccc gtcttgctgc ccaaggaggc
     9901 cccccgggtc gcccggccag agcggccccg agcagacacc ggccatgcct tcctcgccaa
     9961 gcccccagcc cgctccgggc tggagcccgc ctcctccccc agcaagggct cggagccccg
    10021 gcccctagtg cctcctgtct ctggccacgc caccatcgcc cgcacccctg cgaagaacct
    10081 cgcacctcac cacgccagcc cggacccgcc ggcgccacct gcctcggcct cggacccgca
    10141 ccgggaaaag actcaaagta aacccttttc catccaggaa ctggaactcc gttctctggg
    10201 ttaccacggc agcagctaca gccccgaagg ggtggagccc gtcagccctg tgagctcacc
    10261 cagtctgacc cacgacaagg ggctccccaa gcacctggaa gagctcgaca agagccacct
    10321 ggagggggag ctgcggccca agcagccagg ccccgtgaag cttggcgggg aggccgccca
    10381 cctcccacac ctgcggccgc tgcctgagag ccagccctcg tccagcccgc tgctccagac
    10441 cgccccaggg gtcaaaggtc accagcgggt ggtcaccctg gcccagcaca tcagtgaggt
    10501 catcacacag gactacaccc ggcaccaccc acagcagctc agcgcacccc tgcccgcccc
    10561 cctctactcc ttccctgggg ccagctgccc cgtcctggac ctccgccgcc cacccagtga
    10621 cctctacctc ccgcccccgg accatggtgc cccggcccgt ggctcccccc acagcgaagg
    10681 gggcaagagg tctccagagc caaacaagac gtcggtcttg ggtggtggtg aggacggtat
    10741 tgaacctgtg tccccaccgg agggcatgac ggagccaggg cactcccgga gtgctgtgta
    10801 cccgctgctg taccgggatg gggaacagac ggagcccagc aggatgggct ccaagtctcc
    10861 aggcaacacc agccagccgc cagccttctt cagcaagctg accgagagca actccgccat
    10921 ggtcaagtcc aagaagcaag agatcaacaa gaagctgaac acccacaacc ggaatgagcc
    10981 tgaatacaat atcagccagc ctgggacgga gatcttcaat atgcccgcca tcaccggaac
    11041 aggccttatg acctatagaa gccaggcggt gcaggaacat gccagcacca acatggggct
    11101 ggaggccata attagaaagg cactcatggg tggcggcggg aaggccaagg tctctggcag
    11161 acccagcagc cgaaaagcca agtccccggc cccgggcctg gcatctgggg accggccacc
    11221 ctctgtctcc tcagtgcact cggagggaga ctgcaaccgc cggacgccgc tcaccaaccg
    11281 cgtgtgggag gacaggccct cgtccgcagg ttccacgcca ttcccctaca accccctgat
    11341 catgcggctg caggcgggtg tcatggcttc cccaccccca ccgggcctcc ccgcgggcag
    11401 cgggcccctc gctggccccc accacgcctg ggacgaggag cccaagccac tgctctgctc
    11461 gcagtacgag acactctccg acagcgagta gtaatgaact agtccgtaac ttgaaagtat
    11521 ttcgatttct tggctttata tatcttgtgg aaaggacgat gcataggcct tgtattgctt
    11581 gttacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    11641 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    11701 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    11761 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    11821 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    11881 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    11941 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    12001 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    12061 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    12121 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    12181 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    12241 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
    12301 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    12361 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    12421 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    12481 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
    12541 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
    12601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
    12721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    12781 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    12841 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
    12901 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    12961 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    13021 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
    13081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    13141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    13201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    13261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    13321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    13381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    13441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    13501 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
    13561 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    13621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    13681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    13741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    13801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    13861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    13921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    13981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    14041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    14101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    14161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    14221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    14281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    14341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    14401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    14461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    14521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    14581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    14641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    14701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    14761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    14821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    14881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    14941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    15001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    15061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    15121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    15181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    15241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    15301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    15361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    15421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    15481 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    15541 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    15601 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    15661 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    15721 aacaaaagct ggagctgcaa gc
//
